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Literature summary extracted from

  • Gigliobianco, T.; Lakaye, B.; Makarchikov, A.F.; Wins, P.; Bettendorff, L.
    Adenylate kinase-independent thiamine triphosphate accumulation under severe energy stress in Escherichia coli (2008), BMC Microbiol., 8, 16.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.4.3 overexpression in Escherichia coli Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.4.3 ADP + TDP Escherichia coli Escherichia coli adenylate kinase is able to synthesize TTP, but the activity is too low to explain the high rate of TTP accumulation uring amino acid starvation of cells AMP + TTP
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?

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.3 Escherichia coli
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-
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2.7.4.15 Escherichia coli
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BL21 strain and heat-sensitive CV2, CGSC 4682 strain
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Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.4.15 additional information
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thiamine triphoshate accumulation in response to amino acid starvation, controverse mechanism of synthesis dicussed, adenylate kinase-independent accumulation of thiamine triphosphate, heat-sensitive CV2 strain of Escherichia coli accumulates thiamine triphosphate despite full inactivation of adenylate cyclase and subsequent decrease of cellular ATP, ATP-dependent mechanisms for thiamine triphosphate synthesis not favoured since large accumulation also observed during energy stress where ATP is low Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.3 ADP
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Escherichia coli AMP + ATP
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?
2.7.4.3 ADP + TDP Escherichia coli adenylate kinase is able to synthesize TTP, but the activity is too low to explain the high rate of TTP accumulation uring amino acid starvation of cells Escherichia coli AMP + TTP
-
?
2.7.4.3 ADP + TDP rate of TTP synthesis is more than 1000000fold lower than ATP synthesis Escherichia coli AMP + TTP
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?
2.7.4.15 additional information enzymatic synthesis of thiamine triphosphate in Escherichia coli Escherichia coli ?
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?

Synonyms

EC Number Synonyms Comment Organism
2.7.4.15 thiamine diphosphate kinase
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Escherichia coli