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Literature summary extracted from

  • Park, J.S.; Han, K.Y.; Lee, J.H.; Song, J.A.; Ahn, K.Y.; Seo, H.S.; Sim, S.J.; Kim, S.W.; Lee, J.
    Solubility enhancement of aggregation-prone heterologous proteins by fusion expression using stress-responsive Escherichia coli protein, RpoS (2008), BMC Biotechnol., 8, 15.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.5.1.18 biotechnology commercial fusion partner using for enhancing the solubility of recombinant proteins Schistosoma japonicum
3.1.1.74 industry Cutinase is used as a lipolytic enzyme in the composition of laundry and dishwashing detergents to more efficiently remove immobilized fats. The oleochemistry industries and pollutant degradation represent other potential uses of cutinase. Pseudomonas putida

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.18 Expression of recombinant proteins with glutathione S-transferase as fusion expression partner in Escherichia coli strain BL21 (DE3). Schistosoma japonicum
3.1.1.74 Expression of aggregation-prone cutinase protein using RNA polymerase sigma factor (RpoS) or glutathione transferase as fusion partners in Escherichia coli strain BL21 (DE3). Pseudomonas putida
4.1.1.15 Expression of aggregation-prone deletion mutant protein (amino acids 448-585) using RNA polymerase sigma factor (RpoS) or glutathione S-transferase as fusion partners in Escherichia coli strain BL21 (DE3). Homo sapiens

General Stability

EC Number General Stability Organism
3.1.1.74 Using RNA polymerase sigma factor (RpoS) or glutathione transferase as fusion expression partners, the solubility of cutinase significantly increases. Pseudomonas putida
4.1.1.15 using RNA polymerase sigma factor (RpoS) or glutathione transferase as fusion expression partners, the solubility of the enzyme significantly increases. Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.74 additional information Pseudomonas putida Cutinase is known for its hydrolytic activity for a variety of esters ranging from soluble p-nitrophenyl esters to insoluble long-chain triglycerides. The hydrolytic activity of cutinase, especially on p-nitrophenyl esters of fatty acids, is extremely sensitive to fatty acid chain length. ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.18 Schistosoma japonicum
-
 gene from GST descended from Schistosoma containing in vector pET42a(+)
-
3.1.1.74 Pseudomonas putida
-
-
-
4.1.1.15 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.74 purification of his-tagged protein using Ni2+-affinity chromatography Pseudomonas putida

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.18 cell culture
-
 Schistosoma japonicum
-
3.1.1.74 cell culture
-
 Pseudomonas putida
-
4.1.1.15 cell culture
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.74 additional information
-
 Assay of enzymatic activity of the fusion mutant of cutinase (RpoS-CUT) shows the same selective bioactivity as native cutinase to degrade p-nitrophenyl butyrate (PNB) but not to degrade p-nitrophenyl butyrate. Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.18 additional information GST as fusion expression partner enhances the solubility of recombinant proteins Schistosoma japonicum ?
-
 ?
3.1.1.74 additional information Cutinase is known for its hydrolytic activity for a variety of esters ranging from soluble p-nitrophenyl esters to insoluble long-chain triglycerides. The hydrolytic activity of cutinase, especially on p-nitrophenyl esters of fatty acids, is extremely sensitive to fatty acid chain length. Pseudomonas putida ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.18 glutathione transferase
-
 Schistosoma japonicum
2.5.1.18 glutathione-S-transferase
-
 Schistosoma japonicum
2.5.1.18 GST
-
 Schistosoma japonicum
3.1.1.74 cutinase
-
 Pseudomonas putida
4.1.1.15 glutamate decarboxylase
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.74 37
-
 assay at Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.74 8
-
 assay at Pseudomonas putida