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Literature summary extracted from

  • Ely, F.; Nunes, J.E.; Schroeder, E.K.; Frazzon, J.; Palma, M.S.; Santos, D.S.; Basso, L.A.
    The Mycobacterium tuberculosis Rv2540c DNA sequence encodes a bifunctional chorismate synthase (2008), BMC Biochem., 9, 13.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
4.2.3.5 drug development enzymes of shikimate pathway are attractive targets to the development of antitubercular agents Mycobacterium tuberculosis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.5 cloning and expression in Escherichia coli strain Rosetta(DE3) in the absence of IPTG induction, heterologous overexpression of the MtCS protein Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.3.5 41800
-
 determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis and electrospray mass spectrometry Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.5 5-O-(1-carboxyvinyl)-3-phosphoshikimate Mycobacterium tuberculosis seventh enzyme of the shikimate pathway, catalyzes the NADH- and FMN-dependent synthesis of chorismate, a precursor of aromatic amino acids, naphthoquinones, menaquinones, and mycobactins chorismate + phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.5 Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.3.5 by using a 3-step purification (anion exchange, hydrophobic interaction and anion exchange chromatography) Mycobacterium tuberculosis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.3.5 cell culture
-
 Mycobacterium tuberculosis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.3.5 additional information
-
 bifunctionality is determined by measurements of both chorismate synthase and NADH:FMN oxidoreductase activities Mycobacterium tuberculosis
4.2.3.5 additional information
-
 in the presence of oxygen the CS activity is limited by the reoxidation of FMNred cofactor Mycobacterium tuberculosis
4.2.3.5 0.004
-
 CS activity Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.5 5-O-(1-carboxyvinyl)-3-phosphoshikimate seventh enzyme of the shikimate pathway, catalyzes the NADH- and FMN-dependent synthesis of chorismate, a precursor of aromatic amino acids, naphthoquinones, menaquinones, and mycobactins Mycobacterium tuberculosis chorismate + phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
4.2.3.5 dimer in solution, determined by gel filtration chromatography Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
4.2.3.5 5-O-(1-carboxyvinyl)-3-phosphoshikimate phosphate lyase
-
 Mycobacterium tuberculosis
4.2.3.5 aroF
-
 Mycobacterium tuberculosis
4.2.3.5 chorismate synthase
-
 Mycobacterium tuberculosis
4.2.3.5 CS
-
 Mycobacterium tuberculosis
4.2.3.5 MtCS
-
 Mycobacterium tuberculosis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.3.5 25
-
 assay at, CS activity and FMN-reductase activity Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.3.5 0.0083
-
 NADH kinetics of reduction of the MtCS-bound FMNox is determined using fixed amount of holoenzyme and varying levels of NADH Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
4.2.3.5 FMN reduced form Mycobacterium tuberculosis