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Literature summary extracted from
- A novel alkalo- and thermostable phospholipase D from Streptomyces olivochromogenes (2009), Biotechnol. Lett., 31, 429-435.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.4.4 | CHAPS | relative activity: 201 | Streptomyces olivochromogenes |  |
| 3.1.4.4 | deoxycholic acid | relative activity: 246% | Streptomyces olivochromogenes | |
| 3.1.4.4 | polyethylene-4-laurylether | relative activity: 125% | Streptomyces olivochromogenes | |
| 3.1.4.4 | SDS | relative activity: 189 | Streptomyces olivochromogenes | |
| 3.1.4.4 | Triton X-100 | relative activity: 429% | Streptomyces olivochromogenes | |
| 3.1.4.4 | Tween-20 | relative activity: 257% | Streptomyces olivochromogenes | |
| 3.1.4.4 | Tween-80 | relative activity: 249% | Streptomyces olivochromogenes | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.4.4 | 0.6 | - |
phosphatidylcholine | Vmax: 650 micromol/min/mg | Streptomyces olivochromogenes | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.4.4 | Ca2+ | enzyme activity is Ca2+-dependent | Streptomyces olivochromogenes | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.4 | 60000 | - |
SDS-PAGE | Streptomyces olivochromogenes |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.4.4 | Streptomyces olivochromogenes | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.4.4 | using a one-step chromatography on Sepharose CL-6B colum | Streptomyces olivochromogenes |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.4.4 | phosphatidylcholine + glycerol | - |
Streptomyces olivochromogenes | phosphatidylglycerol + choline | - |
? | |
| 3.1.4.4 | phosphatidylcholine + H2O | - |
Streptomyces olivochromogenes | 1,2-diacylglycerophosphate + choline | - |
? | |
| 3.1.4.4 | phosphatidylcholine + L-serine | - |
Streptomyces olivochromogenes | phosphatidylserine + choline | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.4.4 | ? | x * 60000, SDS-PAGE | Streptomyces olivochromogenes |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.4.4 | phospholipase D | - |
Streptomyces olivochromogenes |
| 3.1.4.4 | PLD | - |
Streptomyces olivochromogenes |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.4 | 50 | - |
transphosphatidylation activity highest at | Streptomyces olivochromogenes |
| 3.1.4.4 | 75 | - |
maximal hydrolytic activity | Streptomyces olivochromogenes |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.4 | 55 | 75 | - |
Streptomyces olivochromogenes |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.4 | 8 | - |
hydrolytic and transphosphatidylation activity highest at | Streptomyces olivochromogenes |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.4 | 7 | 13 | - |
Streptomyces olivochromogenes |
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Release 2023.1 (January 2023)
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