EC Number | Application | Comment | Organism |
---|---|---|---|
5.3.1.4 | synthesis | strain carrying mutant C450S/N475K is able to produce 95 g L-ribulose per l from 500 g L-arabinose per l under optimum conditions of pH 8, 70°C, and 10 units enzyme per ml with a conversion yield of 19% over 2 h. The half-lives of the mutated enzyme at 70 and 75°C are 35 and 4.5 h, respectively | Geobacillus thermodenitrificans |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
5.3.1.4 | C450S/N475K | mutant strain is able to produce 95 g L-ribulose per l from 500 g L-arabinose per l under optimum conditions of pH 8, 70°C, and 10 units enzyme per ml with a conversion yield of 19% over 2 h. The half-lives of the mutated enzyme at 70 and 75°C are 35 and 4.5 h, respectively | Geobacillus thermodenitrificans |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.3.1.4 | Geobacillus thermodenitrificans | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.3.1.4 | L-arabinose | equilibrium ratio of L-arabinose to L-ribulose is 88.8:11.2 at 25°C, 80.0:19.0 at 55°C, and 78.0:22.0 at 75°C | Geobacillus thermodenitrificans | L-ribulose | - |
? |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
5.3.1.4 | 70 | - |
half-life 35 h | Geobacillus thermodenitrificans |
5.3.1.4 | 75 | - |
half-life 4.5 h | Geobacillus thermodenitrificans |