BRENDA - Enzyme Database

Cloning and heterologous expression of ectoine biosynthesis genes from Bacillus halodurans in Escherichia coli

Rajan, L.A.; Joseph, T.C.; Thampuran, N.; James, R.; Kumar, K.A.; Viswanathan, C.; Bansal, K.C.; Biotechnol. Lett. 30, 1403-1407 (2008)

Data extracted from this reference:

Application
EC Number
Application
Commentary
Organism
4.2.1.108
biotechnology
ectoine has importance in dermopharmacy as anti ageing agents in skin creams, as components of shampoo, for oral care and as adjuvants for vaccines
Bacillus halodurans
Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
4.2.1.108
the ectABC gene cluster is expressed in Escherichia coli M15 (pREP4) by vector pQE30
Bacillus halodurans
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.108
15000
-
SDS-PAGE, calculated
Bacillus halodurans
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
4.2.1.108
N4-acetyl-L-2,4-diaminobutyrate
Bacillus halodurans
-
2-methyl-1,4,5,6-tetrahydropyrimidine-4-carboxylic acid + H2O
-
-
?
4.2.1.108
N4-acetyl-L-2,4-diaminobutyrate
Bacillus halodurans
-
L-ectoine + H2O
-
-
?
4.2.1.108
N4-acetyl-L-2,4-diaminobutyrate
Bacillus halodurans TCJAR021
-
2-methyl-1,4,5,6-tetrahydropyrimidine-4-carboxylic acid + H2O
-
-
?
4.2.1.108
N4-acetyl-L-2,4-diaminobutyrate
Bacillus halodurans TCJAR021
-
L-ectoine + H2O
-
-
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
4.2.1.108
Bacillus halodurans
A5A5G3
the ectABC gene cluster expressed in Escherichia coli M15 (pREP4)
-
4.2.1.108
Bacillus halodurans TCJAR021
A5A5G3
the ectABC gene cluster expressed in Escherichia coli M15 (pREP4)
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
4.2.1.108
N4-acetyl-L-2,4-diaminobutyrate
-
691437
Bacillus halodurans
2-methyl-1,4,5,6-tetrahydropyrimidine-4-carboxylic acid + H2O
-
-
-
?
4.2.1.108
N4-acetyl-L-2,4-diaminobutyrate
-
691437
Bacillus halodurans TCJAR021
2-methyl-1,4,5,6-tetrahydropyrimidine-4-carboxylic acid + H2O
-
-
-
?
4.2.1.108
N4-acetyl-L-2,4-diaminobutyrate
-
691437
Bacillus halodurans
L-ectoine + H2O
-
-
-
?
4.2.1.108
N4-acetyl-L-2,4-diaminobutyrate
-
691437
Bacillus halodurans TCJAR021
L-ectoine + H2O
-
-
-
?
Synonyms
EC Number
Synonyms
Commentary
Organism
4.2.1.108
ectoine synthase
-
Bacillus halodurans
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
4.2.1.108
Bacillus halodurans
calculated
-
4.93
Application (protein specific)
EC Number
Application
Commentary
Organism
4.2.1.108
biotechnology
ectoine has importance in dermopharmacy as anti ageing agents in skin creams, as components of shampoo, for oral care and as adjuvants for vaccines
Bacillus halodurans
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.108
the ectABC gene cluster is expressed in Escherichia coli M15 (pREP4) by vector pQE30
Bacillus halodurans
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.108
15000
-
SDS-PAGE, calculated
Bacillus halodurans
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
4.2.1.108
N4-acetyl-L-2,4-diaminobutyrate
Bacillus halodurans
-
2-methyl-1,4,5,6-tetrahydropyrimidine-4-carboxylic acid + H2O
-
-
?
4.2.1.108
N4-acetyl-L-2,4-diaminobutyrate
Bacillus halodurans
-
L-ectoine + H2O
-
-
?
4.2.1.108
N4-acetyl-L-2,4-diaminobutyrate
Bacillus halodurans TCJAR021
-
2-methyl-1,4,5,6-tetrahydropyrimidine-4-carboxylic acid + H2O
-
-
?
4.2.1.108
N4-acetyl-L-2,4-diaminobutyrate
Bacillus halodurans TCJAR021
-
L-ectoine + H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
4.2.1.108
N4-acetyl-L-2,4-diaminobutyrate
-
691437
Bacillus halodurans
2-methyl-1,4,5,6-tetrahydropyrimidine-4-carboxylic acid + H2O
-
-
-
?
4.2.1.108
N4-acetyl-L-2,4-diaminobutyrate
-
691437
Bacillus halodurans TCJAR021
2-methyl-1,4,5,6-tetrahydropyrimidine-4-carboxylic acid + H2O
-
-
-
?
4.2.1.108
N4-acetyl-L-2,4-diaminobutyrate
-
691437
Bacillus halodurans
L-ectoine + H2O
-
-
-
?
4.2.1.108
N4-acetyl-L-2,4-diaminobutyrate
-
691437
Bacillus halodurans TCJAR021
L-ectoine + H2O
-
-
-
?
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
4.2.1.108
Bacillus halodurans
calculated
-
4.93