EC Number | Application | Comment | Organism |
---|---|---|---|
4.2.3.20 | drug development | D-limonene is effective in cancer prevention in animal cancer models, some clinical applications have been performed | Nepeta tenuifolia |
EC Number | Cloned (Comment) | Organism |
---|---|---|
4.2.3.20 | expressed in NIH 3T3 cell | Nepeta tenuifolia |
4.2.3.20 | The cDNA of D-limonene synthase obtained from Japanese catnip, modified by deletion of the transit peptide sequence and addition of a new ATG initiation codon, is cloned. Establishment of transgenic mouse embryonic NIH 3T3 fibroblast cells that produce limonene by introducing the D-limonene synthase gene. Apoptosis is not observed in the limonene-producing cells. | Nepeta tenuifolia |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.3.20 | geranyl diphosphate | Nepeta tenuifolia | single step in plants | D-limonene + diphosphate | D-limonene shows cancer preventative and cancer therapeutic activities in vitro and in vivo, its modulates apoptosis-related genes and proteins in cultured cells | ? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.3.20 | Nepeta tenuifolia | Q9FUW5 | - |
- |
4.2.3.20 | Nepeta tenuifolia | Q9FUW5 | production of the monoterpene limonene and modulation of apoptosis-related proteins in embryonic-mouse NIH 3T3 fibroblast cells by introduction of the limonene synthase gene isolated from japanese catnip | - |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
4.2.3.20 | cell culture | - |
Nepeta tenuifolia | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.3.20 | geranyl diphosphate | single step in plants | Nepeta tenuifolia | D-limonene + diphosphate | D-limonene shows cancer preventative and cancer therapeutic activities in vitro and in vivo, its modulates apoptosis-related genes and proteins in cultured cells | ? | |
4.2.3.20 | additional information | a concomitant increase in the level of apoptosis-related protein B-cell lymphoma protein 2 and decreases in the levels of Bcl-2 antagonist of cell death and phosphorylated c-Jun N-terminal kinase are observed in limonene-producing cells | Nepeta tenuifolia | ? | - |
? | |
4.2.3.20 | additional information | limonene can be synthesized in animal cells using their own mevalonate pathway and limonene-producing cells may provide a new system for the elucidation of limonene function | Nepeta tenuifolia | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.2.3.20 | d-limonene synthase | - |
Nepeta tenuifolia |
4.2.3.20 | limonene synthase | - |
Nepeta tenuifolia |