Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Dalal, S.; Singh, P.K.; Raghava, S.; Rawat, S.; Gupta, M.N.
    Purification and properties of the alkaline lipase from Burkholderia cepacia A.T.C.C. 25609 (2008), Biotechnol. Appl. Biochem., 51, 23-31.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.3 olive oil best inducer for the production of lipase Burkholderia cepacia

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.3 expressed in Escherichia coli strain ER2566 Burkholderia cepacia

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.3 11
-
 4-nitrophenyl palmitate at pH 7 and 37°C Burkholderia cepacia
3.1.1.3 16.6
-
 tributyrin at pH 7 and 37°C Burkholderia cepacia

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.3 28000
-
 SDS-PAGE Burkholderia cepacia

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.1.1.3 Triton X-100 stable in the presence of the detergent Triton X-100 Burkholderia cepacia
3.1.1.3 Tween stable in the presence of the detergent Tween 20 Burkholderia cepacia

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Burkholderia cepacia A9QXC9 strain ATCC 25609, formerly called Pseudomonas cepacia
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.3 ammonium sulfate precipitation and Macro-Prep methyl column chromatography Burkholderia cepacia

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.3 230
-
 crude extract, at pH 7 and 37°C Burkholderia cepacia
3.1.1.3 8830
-
 after 38fold purification, at pH 7 and 37°C Burkholderia cepacia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 4-nitrophenyl palmitate + H2O
-
 Burkholderia cepacia 4-nitrophenol + palmitate
-
 ?
3.1.1.3 tributyrin + H2O
-
 Burkholderia cepacia dibutyrin + butyrate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 lipase
-
 Burkholderia cepacia

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.3 40
-
-
 Burkholderia cepacia

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.3 50 60 half-life is 54 and 46 min at 50 and 60°C respectively Burkholderia cepacia

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 9
-
-
 Burkholderia cepacia

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.3 8 10 stable up to 12 h at pH 9 and 10, loses up to 50% activity after 12 h at pH 8 Burkholderia cepacia