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Literature summary extracted from

  • Joseph, B.; Ramteke, P.W.; Thomas, G.
    Cold active microbial lipases: some hot issues and recent developments (2008), Biotechnol. Adv., 26, 457-470.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.1.3 biofuel production conversion of degummed soybean oil to biodiesel fuel, synthesis of lipase-catalyzed biodiesel Aspergillus nidulans
3.1.1.3 biofuel production conversion of degummed soybean oil to biodiesel fuel, synthesis of lipase-catalyzed biodiesel Lysinibacillus sphaericus
3.1.1.3 biofuel production conversion of degummed soybean oil to biodiesel fuel, synthesis of lipase-catalyzed biodiesel Pseudomonas sp.
3.1.1.3 biofuel production conversion of degummed soybean oil to biodiesel fuel, synthesis of lipase-catalyzed biodiesel Serratia marcescens
3.1.1.3 biofuel production conversion of degummed soybean oil to biodiesel fuel, synthesis of lipase-catalyzed biodiesel Acinetobacter sp.
3.1.1.3 biofuel production conversion of degummed soybean oil to biodiesel fuel, synthesis of lipase-catalyzed biodiesel Aeromonas sp.
3.1.1.3 biofuel production conversion of degummed soybean oil to biodiesel fuel, synthesis of lipase-catalyzed biodiesel Psychrobacter sp.
3.1.1.3 biofuel production conversion of degummed soybean oil to biodiesel fuel, synthesis of lipase-catalyzed biodiesel Pseudoalteromonas sp.
3.1.1.3 biofuel production conversion of degummed soybean oil to biodiesel fuel, synthesis of lipase-catalyzed biodiesel Microbacterium phyllosphaerae
3.1.1.3 detergent detergents and cold water washing, production of alpha-butylglucoside lactate by transesterification for cosmetics Aspergillus nidulans
3.1.1.3 detergent detergents and cold water washing, production of alpha-butylglucoside lactate by transesterification for cosmetics Lysinibacillus sphaericus
3.1.1.3 detergent detergents and cold water washing, production of alpha-butylglucoside lactate by transesterification for cosmetics Pseudomonas sp.
3.1.1.3 detergent detergents and cold water washing, production of alpha-butylglucoside lactate by transesterification for cosmetics Serratia marcescens
3.1.1.3 detergent detergents and cold water washing, production of alpha-butylglucoside lactate by transesterification for cosmetics Acinetobacter sp.
3.1.1.3 detergent detergents and cold water washing, production of alpha-butylglucoside lactate by transesterification for cosmetics Aeromonas sp.
3.1.1.3 detergent detergents and cold water washing, production of alpha-butylglucoside lactate by transesterification for cosmetics Psychrobacter sp.
3.1.1.3 detergent detergents and cold water washing, production of alpha-butylglucoside lactate by transesterification for cosmetics Pseudoalteromonas sp.
3.1.1.3 detergent detergents and cold water washing, production of alpha-butylglucoside lactate by transesterification for cosmetics Microbacterium phyllosphaerae
3.1.1.3 environmental protection degradation of lipid wastes, bioremediation and bioaugumentation, removal of solid and water pollution by hydrocarbons, oils and lipids Aspergillus nidulans
3.1.1.3 environmental protection degradation of lipid wastes, bioremediation and bioaugumentation, removal of solid and water pollution by hydrocarbons, oils and lipids Lysinibacillus sphaericus
3.1.1.3 environmental protection degradation of lipid wastes, bioremediation and bioaugumentation, removal of solid and water pollution by hydrocarbons, oils and lipids Pseudomonas sp.
3.1.1.3 environmental protection degradation of lipid wastes, bioremediation and bioaugumentation, removal of solid and water pollution by hydrocarbons, oils and lipids Serratia marcescens
3.1.1.3 environmental protection degradation of lipid wastes, bioremediation and bioaugumentation, removal of solid and water pollution by hydrocarbons, oils and lipids Acinetobacter sp.
3.1.1.3 environmental protection degradation of lipid wastes, bioremediation and bioaugumentation, removal of solid and water pollution by hydrocarbons, oils and lipids Aeromonas sp.
3.1.1.3 environmental protection degradation of lipid wastes, bioremediation and bioaugumentation, removal of solid and water pollution by hydrocarbons, oils and lipids Psychrobacter sp.
3.1.1.3 environmental protection degradation of lipid wastes, bioremediation and bioaugumentation, removal of solid and water pollution by hydrocarbons, oils and lipids Pseudoalteromonas sp.
3.1.1.3 environmental protection degradation of lipid wastes, bioremediation and bioaugumentation, removal of solid and water pollution by hydrocarbons, oils and lipids Microbacterium phyllosphaerae
3.1.1.3 food industry protein polymerization and gelling in fish, improvement in food texture, flavor modification, production of fatty acids and interestrification of fats Aspergillus nidulans
3.1.1.3 food industry protein polymerization and gelling in fish, improvement in food texture, flavor modification, production of fatty acids and interestrification of fats Lysinibacillus sphaericus
3.1.1.3 food industry protein polymerization and gelling in fish, improvement in food texture, flavor modification, production of fatty acids and interestrification of fats Pseudomonas sp.
3.1.1.3 food industry protein polymerization and gelling in fish, improvement in food texture, flavor modification, production of fatty acids and interestrification of fats Serratia marcescens
3.1.1.3 food industry protein polymerization and gelling in fish, improvement in food texture, flavor modification, production of fatty acids and interestrification of fats Acinetobacter sp.
3.1.1.3 food industry protein polymerization and gelling in fish, improvement in food texture, flavor modification, production of fatty acids and interestrification of fats Aeromonas sp.
3.1.1.3 food industry protein polymerization and gelling in fish, improvement in food texture, flavor modification, production of fatty acids and interestrification of fats Psychrobacter sp.
3.1.1.3 food industry protein polymerization and gelling in fish, improvement in food texture, flavor modification, production of fatty acids and interestrification of fats Pseudoalteromonas sp.
3.1.1.3 food industry protein polymerization and gelling in fish, improvement in food texture, flavor modification, production of fatty acids and interestrification of fats Microbacterium phyllosphaerae
3.1.1.3 synthesis synthesis of arylaliphatic glycolipids, ethyl esterification of docosahexaenoic acid to ethyl docosahexaenoate, synthesis of citronellol laurate from citronellol and lauric acid, optically active ester synthesis, ester synthesis, desymmetrization and production of peracids, organic synthesis of chiral intermediates, synthesis of butyl caprylate in n-heptane, synthesis of butyl lactate by transesterification, synthesis of amide Aspergillus nidulans
3.1.1.3 synthesis synthesis of arylaliphatic glycolipids, ethyl esterification of docosahexaenoic acid to ethyl docosahexaenoate, synthesis of citronellol laurate from citronellol and lauric acid, optically active ester synthesis, ester synthesis, desymmetrization and production of peracids, organic synthesis of chiral intermediates, synthesis of butyl caprylate in n-heptane, synthesis of butyl lactate by transesterification, synthesis of amide Lysinibacillus sphaericus
3.1.1.3 synthesis synthesis of arylaliphatic glycolipids, ethyl esterification of docosahexaenoic acid to ethyl docosahexaenoate, synthesis of citronellol laurate from citronellol and lauric acid, optically active ester synthesis, ester synthesis, desymmetrization and production of peracids, organic synthesis of chiral intermediates, synthesis of butyl caprylate in n-heptane, synthesis of butyl lactate by transesterification, synthesis of amide Pseudomonas sp.
3.1.1.3 synthesis synthesis of arylaliphatic glycolipids, ethyl esterification of docosahexaenoic acid to ethyl docosahexaenoate, synthesis of citronellol laurate from citronellol and lauric acid, optically active ester synthesis, ester synthesis, desymmetrization and production of peracids, organic synthesis of chiral intermediates, synthesis of butyl caprylate in n-heptane, synthesis of butyl lactate by transesterification, synthesis of amide Serratia marcescens
3.1.1.3 synthesis synthesis of arylaliphatic glycolipids, ethyl esterification of docosahexaenoic acid to ethyl docosahexaenoate, synthesis of citronellol laurate from citronellol and lauric acid, optically active ester synthesis, ester synthesis, desymmetrization and production of peracids, organic synthesis of chiral intermediates, synthesis of butyl caprylate in n-heptane, synthesis of butyl lactate by transesterification, synthesis of amide Acinetobacter sp.
3.1.1.3 synthesis synthesis of arylaliphatic glycolipids, ethyl esterification of docosahexaenoic acid to ethyl docosahexaenoate, synthesis of citronellol laurate from citronellol and lauric acid, optically active ester synthesis, ester synthesis, desymmetrization and production of peracids, organic synthesis of chiral intermediates, synthesis of butyl caprylate in n-heptane, synthesis of butyl lactate by transesterification, synthesis of amide Aeromonas sp.
3.1.1.3 synthesis synthesis of arylaliphatic glycolipids, ethyl esterification of docosahexaenoic acid to ethyl docosahexaenoate, synthesis of citronellol laurate from citronellol and lauric acid, optically active ester synthesis, ester synthesis, desymmetrization and production of peracids, organic synthesis of chiral intermediates, synthesis of butyl caprylate in n-heptane, synthesis of butyl lactate by transesterification, synthesis of amide Psychrobacter sp.
3.1.1.3 synthesis synthesis of arylaliphatic glycolipids, ethyl esterification of docosahexaenoic acid to ethyl docosahexaenoate, synthesis of citronellol laurate from citronellol and lauric acid, optically active ester synthesis, ester synthesis, desymmetrization and production of peracids, organic synthesis of chiral intermediates, synthesis of butyl caprylate in n-heptane, synthesis of butyl lactate by transesterification, synthesis of amide Pseudoalteromonas sp.
3.1.1.3 synthesis synthesis of arylaliphatic glycolipids, ethyl esterification of docosahexaenoic acid to ethyl docosahexaenoate, synthesis of citronellol laurate from citronellol and lauric acid, optically active ester synthesis, ester synthesis, desymmetrization and production of peracids, organic synthesis of chiral intermediates, synthesis of butyl caprylate in n-heptane, synthesis of butyl lactate by transesterification, synthesis of amide Microbacterium phyllosphaerae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.3
-
Psychrobacter sp.
3.1.1.3 expressed in Escherichia coli Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.3 Cu2+ strong inhibition Pseudomonas sp.
3.1.1.3 Fe3+ strong inhibition Pseudomonas sp.
3.1.1.3 Hg2+ strong inhibition Pseudomonas sp.
3.1.1.3 Zn2+ strong inhibition Pseudomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.3 Ca2+ enhances activity Psychrobacter sp.
3.1.1.3 Mg2+ enhances activity Psychrobacter sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.3 29000
-
-
Aspergillus nidulans
3.1.1.3 33700
-
-
Pseudomonas sp.
3.1.1.3 40000
-
SDS-PAGE Lysinibacillus sphaericus
3.1.1.3 42000
-
-
Microbacterium phyllosphaerae
3.1.1.3 49900
-
-
Pseudomonas sp.
3.1.1.3 50000
-
SDS-PAGE Aeromonas sp.
3.1.1.3 52000
-
-
Serratia marcescens
3.1.1.3 85000
-
-
Psychrobacter sp.
3.1.1.3 85000
-
-
Pseudoalteromonas sp.

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Acinetobacter sp.
-
strain no. 6
-
3.1.1.3 Aeromonas sp.
-
-
-
3.1.1.3 Aeromonas sp. LPB 4
-
-
-
3.1.1.3 Aspergillus nidulans
-
-
-
3.1.1.3 Aspergillus nidulans WG312
-
-
-
3.1.1.3 Lysinibacillus sphaericus
-
-
-
3.1.1.3 Lysinibacillus sphaericus MTCC 7526
-
-
-
3.1.1.3 Microbacterium phyllosphaerae
-
-
-
3.1.1.3 Microbacterium phyllosphaerae MTCC 7530
-
-
-
3.1.1.3 Pseudoalteromonas sp.
-
-
-
3.1.1.3 Pseudoalteromonas sp. wp27
-
-
-
3.1.1.3 Pseudomonas sp.
-
-
-
3.1.1.3 Pseudomonas sp. B11-1
-
-
-
3.1.1.3 Pseudomonas sp. KB700A
-
-
-
3.1.1.3 Psychrobacter sp.
-
-
-
3.1.1.3 Psychrobacter sp. 7195
-
-
-
3.1.1.3 Psychrobacter sp. wp37
-
-
-
3.1.1.3 Serratia marcescens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.3 CM-cellulose column chromatography, DEAE cellulose column chromatography, and Sephadex G-150 gel filtration Serratia marcescens
3.1.1.3 DEAE cellulofine chromatography Pseudomonas sp.
3.1.1.3 DEAE cellulose column chromatography Lysinibacillus sphaericus
3.1.1.3 DEAE cellulose column chromatography Pseudomonas sp.
3.1.1.3 DEAE cellulose column chromatography Microbacterium phyllosphaerae
3.1.1.3 DEAE Sepharose CL-4B column chromatography and Sephadex G-75 gel filtration Psychrobacter sp.
3.1.1.3 phenyl-Sepharose column chromatography and linolenic acid-agarose chromatography Aspergillus nidulans
3.1.1.3 QAE-Sephadex gel filtration Aeromonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 additional information broad specificity towards the acyl group (C8-C16) of ethyl esters Acinetobacter sp. ?
-
?
3.1.1.3 additional information medium chain acyl group p-nitrophenyl esters are good substrate, increased activity with detergents Aeromonas sp. ?
-
?
3.1.1.3 additional information preference toward esters of short- and middle-chain fatty acids Aspergillus nidulans ?
-
?
3.1.1.3 additional information preference toward esters of short- and middle-chain fatty acids Aspergillus nidulans WG312 ?
-
?
3.1.1.3 additional information medium chain acyl group p-nitrophenyl esters are good substrate, increased activity with detergents Aeromonas sp. LPB 4 ?
-
?
3.1.1.3 p-nitrophenyl caprate + H2O highest activity with p-nitrophenyl caprate Pseudomonas sp. p-nitrophenol + caprate
-
?
3.1.1.3 p-nitrophenyl caprate + H2O highest activity with p-nitrophenyl caprate Pseudomonas sp. KB700A p-nitrophenol + caprate
-
?
3.1.1.3 p-nitrophenyl caprate + H2O highest activity with p-nitrophenyl caprate Pseudomonas sp. B11-1 p-nitrophenol + caprate
-
?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 triacylglycerol acyl hydrolase
-
Aspergillus nidulans
3.1.1.3 triacylglycerol acyl hydrolase
-
Lysinibacillus sphaericus
3.1.1.3 triacylglycerol acyl hydrolase
-
Pseudomonas sp.
3.1.1.3 triacylglycerol acyl hydrolase
-
Serratia marcescens
3.1.1.3 triacylglycerol acyl hydrolase
-
Acinetobacter sp.
3.1.1.3 triacylglycerol acyl hydrolase
-
Aeromonas sp.
3.1.1.3 triacylglycerol acyl hydrolase
-
Psychrobacter sp.
3.1.1.3 triacylglycerol acyl hydrolase
-
Pseudoalteromonas sp.
3.1.1.3 triacylglycerol acyl hydrolase
-
Microbacterium phyllosphaerae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.3 15
-
-
Lysinibacillus sphaericus
3.1.1.3 20
-
-
Acinetobacter sp.
3.1.1.3 20
-
-
Microbacterium phyllosphaerae
3.1.1.3 20 30
-
Psychrobacter sp.
3.1.1.3 20 30
-
Pseudoalteromonas sp.
3.1.1.3 30
-
-
Psychrobacter sp.
3.1.1.3 35
-
-
Pseudomonas sp.
3.1.1.3 35
-
-
Aeromonas sp.
3.1.1.3 37
-
-
Serratia marcescens
3.1.1.3 40
-
-
Aspergillus nidulans
3.1.1.3 45
-
-
Pseudomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.3 4
-
enzyme is 60% active at 4°C Pseudomonas sp.
3.1.1.3 4
-
enzyme is 60% active at 4°C Psychrobacter sp.
3.1.1.3 4
-
enzyme is 60% active at 4°C Pseudoalteromonas sp.
3.1.1.3 5 35
-
Pseudomonas sp.
3.1.1.3 5 65 90% activity at 5°C, remains stable at 65°C Serratia marcescens
3.1.1.3 30
-
stable at 30°C Lysinibacillus sphaericus
3.1.1.3 35
-
stable at 35°C Microbacterium phyllosphaerae
3.1.1.3 50
-
stable at 50°C Aeromonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 6.5
-
-
Aspergillus nidulans
3.1.1.3 7 8
-
Psychrobacter sp.
3.1.1.3 7 8
-
Pseudoalteromonas sp.
3.1.1.3 8
-
-
Lysinibacillus sphaericus
3.1.1.3 8
-
-
Pseudomonas sp.
3.1.1.3 8
-
-
Serratia marcescens
3.1.1.3 8
-
-
Microbacterium phyllosphaerae
3.1.1.3 8 8.5
-
Pseudomonas sp.
3.1.1.3 9
-
-
Psychrobacter sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.3 6 9
-
Pseudomonas sp.
3.1.1.3 6.6
-
remains stable at pH 6.6 Serratia marcescens
3.1.1.3 7 10
-
Psychrobacter sp.
3.1.1.3 8
-
stable at pH 8 Lysinibacillus sphaericus
3.1.1.3 8
-
stable at pH 8 Microbacterium phyllosphaerae