EC Number | Cloned (Comment) | Organism |
---|---|---|
4.2.1.109 | expressed as (His)6-tagged soluble protein in Escherichia BL21 (DE3), vector pET15b | Bacillus subtilis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.1.109 | 0.0089 | - |
S-methyl-5-thio-D-ribulose 1-phosphate | 25°C | Bacillus subtilis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.2.1.109 | additional information | MtnB requires metal ions for catalysis, but it is unclear what kind of metal ions are bound to the MtnB purified from Escherichia coli cells. Considering that FucA/RibE/RhuA-related enzymes require the zinc ion for optimal catalysis under physiological conditions and that recombinant class II aldolase enzymes expressed in Escherichia coli cells are almost all of the Zn2+ form, purified MtnB might also be of the Zn2+ form | Bacillus subtilis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.2.1.109 | 23800 | - |
4 * 23800 | Bacillus subtilis |
4.2.1.109 | 90000 | - |
without His-tag, 4 * 23800, SDS-PAGE | Bacillus subtilis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.109 | S-methyl-5-thio-D-ribulose 1-phosphate | Bacillus subtilis | - |
5-(methylthio)-2,3-dioxopentyl phosphate + H2O | - |
? | |
4.2.1.109 | S-methyl-5-thio-D-ribulose 1-phosphate | Bacillus subtilis 168 | - |
5-(methylthio)-2,3-dioxopentyl phosphate + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.1.109 | Bacillus subtilis | O31668 | trp C2 | - |
4.2.1.109 | Bacillus subtilis 168 | O31668 | trp C2 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.2.1.109 | homogeneity, using the His-tag, the His-tag is cleaved with thrombin | Bacillus subtilis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.109 | S-methyl-5-thio-D-ribulose 1-phosphate | - |
Bacillus subtilis | 5-(methylthio)-2,3-dioxopentyl phosphate + H2O | - |
? | |
4.2.1.109 | S-methyl-5-thio-D-ribulose 1-phosphate | - |
Bacillus subtilis | 5-(methylthio)-2,3-dioxopentyl phosphate + H2O | the reaction product 5-(methylthio)-2,3-dioxopentyl phosphate is so labile that its chemical structure could not be isolated and analyzed | ? | |
4.2.1.109 | S-methyl-5-thio-D-ribulose 1-phosphate | - |
Bacillus subtilis 168 | 5-(methylthio)-2,3-dioxopentyl phosphate + H2O | - |
? | |
4.2.1.109 | S-methyl-5-thio-D-ribulose 1-phosphate | - |
Bacillus subtilis 168 | 5-(methylthio)-2,3-dioxopentyl phosphate + H2O | the reaction product 5-(methylthio)-2,3-dioxopentyl phosphate is so labile that its chemical structure could not be isolated and analyzed | ? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.2.1.109 | homotetramer | 4 * 23800 | Bacillus subtilis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.2.1.109 | 5-Methylthioribulose-1-phosphate | - |
Bacillus subtilis |
4.2.1.109 | MtnB | - |
Bacillus subtilis |
4.2.1.109 | MTRu-1-P dehydratase | - |
Bacillus subtilis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.1.109 | 40 | - |
at 55°C the enzyme loses most of its activity | Bacillus subtilis |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.1.109 | 15 | 55 | at 55°C the enzyme loses most of its activity | Bacillus subtilis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.1.109 | 16.5 | - |
S-methyl-5-thio-D-ribulose 1-phosphate | - |
Bacillus subtilis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.2.1.109 | 7.5 | 8 | - |
Bacillus subtilis |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
4.2.1.109 | 6.5 | 10.5 | - |
Bacillus subtilis |