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Literature summary extracted from

  • Kawai, S.; Murata, K.
    Structure and function of NAD kinase and NADP phosphatase: key enzymes that regulate the intracellular balance of NAD(H) and NADP(H) (2008), Biosci. Biotechnol. Biochem., 72, 919-930.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.23 Calmodulin Ca2+/calmodulin regulates NAD kinase activity. Ca2+/calmodulin has no detectable effect on recombinant human NAD kinase Homo sapiens
2.7.1.23 H2O2 upregulates NADK1 by 3fold after treatment of plant cells with 5 mM Arabidopsis thaliana
2.7.1.23 additional information irradiation upregulates NADK1 by 8fold. NADK1 is Ca2+/calmodulin-independent, and not activated by Ca2+/calmodulin Arabidopsis thaliana
2.7.1.23 additional information NADK2 is Ca2+/calmodulin-independent, and not activated by Ca2+/calmodulin, although recombinant NADK2, which has a calmodulin-binding motif in its N-terminal, is able to bind to calmodulin Arabidopsis thaliana
2.7.1.23 additional information NADK3 is Ca2+/calmodulin-independent, and not activated by Ca2+/calmodulin Arabidopsis thaliana
2.7.1.23 additional information is not regulated by NADH Micrococcus luteus

Application

EC Number Application Comment Organism
2.7.1.23 additional information one of the key enzymes regulating the balance of NAD(H) and NADP(H) Mycobacterium tuberculosis
2.7.1.23 additional information one of the key enzymes regulating the balance of NAD(H) and NADP(H) Schizosaccharomyces pombe
2.7.1.23 additional information one of the key enzymes regulating the balance of NAD(H) and NADP(H) Homo sapiens
2.7.1.23 additional information one of the key enzymes regulating the balance of NAD(H) and NADP(H) Methanocaldococcus jannaschii
2.7.1.23 additional information one of the key enzymes regulating the balance of NAD(H) and NADP(H) Pyrococcus horikoshii
2.7.1.23 additional information one of the key enzymes regulating the balance of NAD(H) and NADP(H) Bacillus subtilis
2.7.1.23 additional information one of the key enzymes regulating the balance of NAD(H) and NADP(H) Escherichia coli
2.7.1.23 additional information one of the key enzymes regulating the balance of NAD(H) and NADP(H) Sphingomonas sp. A1
2.7.1.23 additional information one of the key enzymes regulating the balance of NAD(H) and NADP(H) Archaeoglobus fulgidus
2.7.1.23 additional information one of the key enzymes regulating the balance of NAD(H) and NADP(H) Arabidopsis thaliana
2.7.1.23 additional information one of the key enzymes regulating the balance of NAD(H) and NADP(H) Synechococcus elongatus PCC 6301
2.7.1.23 additional information one of the key enzymes regulating the balance of NAD(H) and NADP(H) Candida albicans
2.7.1.23 additional information one of the key enzymes regulating the balance of NAD(H) and NADP(H) Dictyostelium discoideum
2.7.1.23 additional information one of the key enzymes regulating the balance of NAD(H) and NADP(H) Salmonella enterica
2.7.1.23 additional information one of the key enzymes regulating the balance of NAD(H) and NADP(H) Oryza sativa
2.7.1.23 additional information one of the key enzymes regulating the balance of NAD(H) and NADP(H) Lactuca sativa
2.7.1.23 additional information one of the key enzymes regulating the balance of NAD(H) and NADP(H) Glycine max
2.7.1.23 additional information one of the key enzymes regulating the balance of NAD(H) and NADP(H) Triticum sp.
2.7.1.23 additional information one of the key enzymes regulating the balance of NAD(H) and NADP(H) Sorghum sp.
2.7.1.23 additional information one of the key enzymes regulating the balance of NAD(H) and NADP(H) Listeria monocytogenes
2.7.1.23 additional information one of the key enzymes regulating the balance of NAD(H) and NADP(H) Thermotoga maritima
2.7.1.23 additional information one of the key enzymes regulating the balance of NAD(H) and NADP(H) Saccharomyces cerevisiae
2.7.1.23 additional information one of the key enzymes regulating the balance of NAD(H) and NADP(H) Columba livia
2.7.1.23 additional information one of the key enzymes regulating the balance of NAD(H) and NADP(H) Cyberlindnera jadinii
2.7.1.23 additional information one of the key enzymes regulating the balance of NAD(H) and NADP(H) Micrococcus luteus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.23
-
 Arabidopsis thaliana
2.7.1.23
-
 Homo sapiens
2.7.1.23
-
 Methanocaldococcus jannaschii
2.7.1.23 overexpression of UTR1 via a high-copy plasmid Saccharomyces cerevisiae
2.7.1.23 overexpression of YEF1 via a high-copy plasmid Saccharomyces cerevisiae
2.7.1.23 Ppnk overexpressed Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.23 apo form of Ppnk at 2.8 A resolution. Crystals belong to space group C2 (a=140.2, b=69.4, c=106.4 A, and beta=130°). Holo form of Ppnk complexed with NAD+ at 2.6 A resolution. Crystals belong space group to P62 (a=b=110.5 and c=108.9 A). Overall structures of apo-Ppnk and Ppnk-NAD consist of an N-domain (residues 1-138 and 279-283), a C-domain (residues 139-278), and a C-terminal tail (residues 284-307) Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.23 additional information mutants of the NAD kinase gene are lethal Mycobacterium tuberculosis
2.7.1.23 additional information sensitivity of NADK(-) cells to oxidative stress is the same as that of the control cells Homo sapiens
2.7.1.23 additional information mutant for NadF is lethal Bacillus subtilis
2.7.1.23 additional information NADK1-deficient mutant exhibits sensitivity to gamma irradiation and paraquat-induced oxidative stress Arabidopsis thaliana
2.7.1.23 additional information NADK2-deficient mutant grows slowly, is sensitive to environmental stress, which leads to oxidative stress, and shows reduced chlorophyll content. NADK1-deficient mutant exhibits sensitivity to gamma irradiation and paraquat-induced oxidative stress Arabidopsis thaliana
2.7.1.23 additional information mutants of the NAD kinase gene are lethal Salmonella enterica
2.7.1.23 additional information triple mutant utr1yef1pos5 is lethal. Double mutant utr1pos5 is lethal against the SEY6210.5 and S288c backgrounds Saccharomyces cerevisiae
2.7.1.23 additional information triple mutant utr1yef1pos5 is lethal Saccharomyces cerevisiae
2.7.1.23 additional information triple mutant utr1yef1pos5 is lethal. Double mutant utr1pos5 is lethal against the SEY6210.5 and S288c backgrounds. Mutant for POS5 shows several phenotypes related to decreased NADPH concentration in the mitochondria, and consequently, decreased mitochondrial function Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.23 additional information utr1 shows slow growth in a low-iron medium Saccharomyces cerevisiae
2.7.1.23 NADH
-
 Escherichia coli
2.7.1.23 NADH
-
 Salmonella enterica
2.7.1.23 NADPH
-
 Escherichia coli
2.7.1.23 NADPH
-
 Homo sapiens
2.7.1.23 NADPH
-
 Salmonella enterica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.23 0.18
-
 NADP+
-
 Methanocaldococcus jannaschii
2.7.1.23 0.3
-
 NADPH
-
 Methanocaldococcus jannaschii
2.7.1.23 0.43
-
 fructose-1,6-bisphosphate
-
 Methanocaldococcus jannaschii
3.1.3.11 0.43
-
 D-fructose 1,6-bisphosphate pH and temperature not specified in the publication Methanocaldococcus jannaschii
3.1.3.11 0.43
-
 D-fructose 1,6-bisphosphate pH and temperature not specified in the publication Archaeoglobus fulgidus
3.1.3.11 0.73
-
 D-fructose 1,6-bisphosphate pH and temperature not specified in the publication Methanocaldococcus jannaschii
3.1.3.108 0.14
-
 NADP+ pH and temperature not specified in the publication Archaeoglobus fulgidus
3.1.3.108 0.18
-
 NADP+ pH and temperature not specified in the publication Methanocaldococcus jannaschii
3.1.3.108 0.3
-
 NADPH pH and temperature not specified in the publication Methanocaldococcus jannaschii
3.1.3.108 0.36
-
 NADPH pH and temperature not specified in the publication Archaeoglobus fulgidus
3.1.3.108 0.54
-
 NADP+ pH and temperature not specified in the publication Methanocaldococcus jannaschii
3.1.3.108 0.77
-
 NADPH pH and temperature not specified in the publication Methanocaldococcus jannaschii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.23 chloroplast
-
 Arabidopsis thaliana 9507
-
2.7.1.23 chloroplast isoform NADK2 Arabidopsis thaliana 9507
-
2.7.1.23 cytoplasm
-
 Homo sapiens 5737
-
2.7.1.23 cytosol
-
 Saccharomyces cerevisiae 5829
-
2.7.1.23 mitochondrion
-
 Saccharomyces cerevisiae 5739
-
2.7.1.86 mitochondrion
-
 Saccharomyces cerevisiae 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.23 Ca2+ Ca2+/calmodulin-binding domain Glycine max
2.7.1.23 Ca2+ Ca2+/calmodulin regulates NAD kinase activity. Ca2+/calmodulin has no detectable effect on recombinant human NAD kinase Homo sapiens
2.7.1.23 Ca2+ Ca2+/calmodulin-binding domain Lactuca sativa
2.7.1.23 Ca2+ Ca2+/calmodulin-binding domain Oryza sativa
2.7.1.23 Ca2+ Ca2+/calmodulin-binding domain Sorghum sp.
2.7.1.23 Ca2+ Ca2+/calmodulin-binding domain Triticum sp.
2.7.1.23 Cu2+ POS5 is upregulated 3.4fold after treatment for 10 to 12 min with 2.5 mM Saccharomyces cerevisiae
2.7.1.23 additional information NADK1 is Ca2+/calmodulin-independent, and not activated by Ca2+/calmodulin Arabidopsis thaliana
2.7.1.23 additional information NADK2 is Ca2+/calmodulin-independent, and not activated by Ca2+/calmodulin Arabidopsis thaliana
2.7.1.23 additional information NADK3 is Ca2+/calmodulin-independent, and not activated by Ca2+/calmodulin Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.23 30000
-
 2 * 30000 Bacillus subtilis
2.7.1.23 32000
-
 2 * 32000 Sphingomonas sp. A1
2.7.1.23 37000
-
 4 * 37000 Pyrococcus horikoshii
2.7.1.23 46000
-
 x * 46000 Saccharomyces cerevisiae
2.7.1.23 49000
-
 4 * 49000 Homo sapiens
2.7.1.23 60000
-
 6 * 60000 Saccharomyces cerevisiae
2.7.1.23 60000
-
 8 * 60000 Saccharomyces cerevisiae
2.7.1.23 60000
-
 6 * 60000, Utr1p Saccharomyces cerevisiae
2.7.1.23 60000
-
 6 * 60000, Yef1p Saccharomyces cerevisiae
2.7.1.23 110000
-
 isoform NADK2 Arabidopsis thaliana
2.7.1.86 46000
-
-
 Saccharomyces cerevisiae
2.7.1.86 58000
-
 isoform NADK1 Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.11 D-fructose 1,6-bisphosphate + H2O Methanocaldococcus jannaschii
-
 D-fructose 6-phosphate + phosphate
-
 ?
3.1.3.11 D-fructose 1,6-bisphosphate + H2O Methanocaldococcus jannaschii ATCC 43067
-
 D-fructose 6-phosphate + phosphate
-
 ?
3.1.3.108 NADP+ + H2O Methanocaldococcus jannaschii the enzyme regulates the intracellular balance of NAD(H) and NADP(H) NAD+ + phosphate
-
 ?
3.1.3.108 NADP+ + H2O Archaeoglobus fulgidus the enzyme regulates the intracellular balance of NAD(H) and NADP(H) NAD+ + phosphate
-
 ?
3.1.3.108 NADP+ + H2O Methanocaldococcus jannaschii ATCC 43067 the enzyme regulates the intracellular balance of NAD(H) and NADP(H) NAD+ + phosphate
-
 ?
3.1.3.108 NADP+ + H2O Archaeoglobus fulgidus ATCC 49558 the enzyme regulates the intracellular balance of NAD(H) and NADP(H) NAD+ + phosphate
-
 ?
3.1.3.108 NADPH + H2O Methanocaldococcus jannaschii the enzyme regulates the intracellular balance of NAD(H) and NADP(H) NADH + phosphate
-
 ?
3.1.3.108 NADPH + H2O Archaeoglobus fulgidus the enzyme regulates the intracellular balance of NAD(H) and NADP(H) NADH + phosphate
-
 ?
3.1.3.108 NADPH + H2O Methanocaldococcus jannaschii ATCC 43067 the enzyme regulates the intracellular balance of NAD(H) and NADP(H) NADH + phosphate
-
 ?
3.1.3.108 NADPH + H2O Archaeoglobus fulgidus ATCC 49558 the enzyme regulates the intracellular balance of NAD(H) and NADP(H) NADH + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.23 Arabidopsis thaliana
-
-
-
2.7.1.23 Arabidopsis thaliana Q56YN3
-
-
2.7.1.23 Arabidopsis thaliana Q9C5W3
-
-
2.7.1.23 Arabidopsis thaliana Q500Y9
-
-
2.7.1.23 Archaeoglobus fulgidus
-
-
-
2.7.1.23 Bacillus subtilis O31612
-
-
2.7.1.23 Bacillus subtilis O34934
-
-
2.7.1.23 Candida albicans
-
-
-
2.7.1.23 Columba livia
-
-
-
2.7.1.23 Cyberlindnera jadinii
-
-
-
2.7.1.23 Dictyostelium discoideum
-
-
-
2.7.1.23 Escherichia coli P0A7B3
-
-
2.7.1.23 Glycine max
-
-
-
2.7.1.23 Homo sapiens
-
-
-
2.7.1.23 Homo sapiens O95544
-
-
2.7.1.23 Lactuca sativa
-
-
-
2.7.1.23 Listeria monocytogenes
-
-
-
2.7.1.23 Methanocaldococcus jannaschii Q58327
-
-
2.7.1.23 Micrococcus luteus Q8VUL9
-
-
2.7.1.23 Mycobacterium tuberculosis P9WHV7
-
-
2.7.1.23 Mycobacterium tuberculosis H37Rv P9WHV7
-
-
2.7.1.23 Oryza sativa
-
-
-
2.7.1.23 Pyrococcus horikoshii O58801
-
-
2.7.1.23 Saccharomyces cerevisiae P21373
-
-
2.7.1.23 Saccharomyces cerevisiae P32622
-
-
2.7.1.23 Saccharomyces cerevisiae Q06892
-
-
2.7.1.23 Saccharomyces cerevisiae C7GKE4
-
-
2.7.1.23 Salmonella enterica
-
-
-
2.7.1.23 Schizosaccharomyces pombe
-
-
-
2.7.1.23 Sorghum sp.
-
-
-
2.7.1.23 Sphingomonas sp. A1 Q6L7J5
-
-
2.7.1.23 Synechococcus elongatus PCC 6301
-
-
-
2.7.1.23 Thermotoga maritima
-
-
-
2.7.1.23 Triticum sp.
-
-
-
2.7.1.86 Arabidopsis thaliana
-
-
-
2.7.1.86 Saccharomyces cerevisiae C7GJD6
-
-
3.1.3.11 Archaeoglobus fulgidus O30298 cf. EC 3.1.3.108, EC 3.1.3.25
-
3.1.3.11 Archaeoglobus fulgidus ATCC 49558 O30298 cf. EC 3.1.3.108, EC 3.1.3.25
-
3.1.3.11 Methanocaldococcus jannaschii Q58327 cf. EC 2.7.1.23 and EC 3.1.3.108
-
3.1.3.11 Methanocaldococcus jannaschii Q57573 cf. EC 3.1.3.108
-
3.1.3.11 Methanocaldococcus jannaschii ATCC 43067 Q58327 cf. EC 2.7.1.23 and EC 3.1.3.108
-
3.1.3.11 Methanocaldococcus jannaschii ATCC 43067 Q57573 cf. EC 3.1.3.108
-
3.1.3.108 Archaeoglobus fulgidus O30298 cf. EC 3.1.3.11, EC 3.1.3.25
-
3.1.3.108 Archaeoglobus fulgidus ATCC 49558 O30298 cf. EC 3.1.3.11, EC 3.1.3.25
-
3.1.3.108 Methanocaldococcus jannaschii Q58327 cf. EC 2.7.1.23
-
3.1.3.108 Methanocaldococcus jannaschii Q57573 cf. EC 3.1.3.11
-
3.1.3.108 Methanocaldococcus jannaschii ATCC 43067 Q58327 cf. EC 2.7.1.23
-
3.1.3.108 Methanocaldococcus jannaschii ATCC 43067 Q57573 cf. EC 3.1.3.11
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.23
-
 Columba livia
2.7.1.23
-
 Cyberlindnera jadinii
2.7.1.23
-
 Homo sapiens
2.7.1.23
-
 Methanocaldococcus jannaschii
2.7.1.23 partially purified Arabidopsis thaliana
2.7.1.23 partially purified Saccharomyces cerevisiae
2.7.1.23 Rv1695 protein Mycobacterium tuberculosis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.23 heart
-
 Columba livia
-
2.7.1.23 liver
-
 Columba livia
-
2.7.1.23 neutrophil
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.23 ATP + NAD+
-
 Mycobacterium tuberculosis ADP + NADP+
-
 ?
2.7.1.23 ATP + NAD+
-
 Homo sapiens ADP + NADP+
-
 ?
2.7.1.23 ATP + NAD+
-
 Methanocaldococcus jannaschii ADP + NADP+
-
 ?
2.7.1.23 ATP + NAD+
-
 Pyrococcus horikoshii ADP + NADP+
-
 ?
2.7.1.23 ATP + NAD+
-
 Bacillus subtilis ADP + NADP+
-
 ?
2.7.1.23 ATP + NAD+
-
 Escherichia coli ADP + NADP+
-
 ?
2.7.1.23 ATP + NAD+
-
 Sphingomonas sp. A1 ADP + NADP+
-
 ?
2.7.1.23 ATP + NAD+
-
 Arabidopsis thaliana ADP + NADP+
-
 ?
2.7.1.23 ATP + NAD+
-
 Salmonella enterica ADP + NADP+
-
 ?
2.7.1.23 ATP + NAD+
-
 Saccharomyces cerevisiae ADP + NADP+
-
 ?
2.7.1.23 ATP + NAD+
-
 Micrococcus luteus ADP + NADP+
-
 ?
2.7.1.23 ATP + NAD+ 100% activity Arabidopsis thaliana ADP + NADP+
-
 ?
2.7.1.23 ATP + NAD+
-
 Mycobacterium tuberculosis H37Rv ADP + NADP+
-
 ?
2.7.1.23 ATP + NAD+
-
 Methanocaldococcus jannaschii ATCC 43067 ADP + NADP+
-
 ?
2.7.1.23 ATP + NADH low activity Mycobacterium tuberculosis ADP + NADPH
-
 ?
2.7.1.23 ATP + NADH
-
 Homo sapiens ADP + NADPH
-
 ?
2.7.1.23 ATP + NADH
-
 Methanocaldococcus jannaschii ADP + NADPH
-
 ?
2.7.1.23 ATP + NADH
-
 Pyrococcus horikoshii ADP + NADPH
-
 ?
2.7.1.23 ATP + NADH
-
 Arabidopsis thaliana ADP + NADPH
-
 ?
2.7.1.23 ATP + NADH low activity with NADH Saccharomyces cerevisiae ADP + NADPH
-
 ?
2.7.1.23 ATP + NADH higher activity with NADH than with NAD+ Saccharomyces cerevisiae ADP + NADPH
-
 ?
2.7.1.23 ATP + NADH
-
 Micrococcus luteus ADP + NADPH
-
 ?
2.7.1.23 ATP + NADH while isoform Yef1p displays 15% activity with NADH compared to NAD+ Saccharomyces cerevisiae ADP + NADPH
-
 ?
2.7.1.23 ATP + NADH isoform Utr1p displays 15% activity with NADH compared to NAD+ Saccharomyces cerevisiae ADP + NADPH
-
 ?
2.7.1.23 ATP + NADH low activity Mycobacterium tuberculosis H37Rv ADP + NADPH
-
 ?
2.7.1.23 ATP + NADH
-
 Methanocaldococcus jannaschii ATCC 43067 ADP + NADPH
-
 ?
2.7.1.23 fructose-1,6-bisphosphate + ?
-
 Methanocaldococcus jannaschii ?
-
 ?
2.7.1.23 fructose-1,6-bisphosphate + ?
-
 Methanocaldococcus jannaschii ATCC 43067 ?
-
 ?
2.7.1.23 additional information absence of any NADH-phosphorylating (NADH kinase) activity of NAD kinase Salmonella enterica ?
-
 ?
2.7.1.23 additional information no activity with poly(P) Saccharomyces cerevisiae ?
-
 ?
2.7.1.23 additional information does not accept poly(P) as phosphoryl donor Saccharomyces cerevisiae ?
-
 ?
2.7.1.23 additional information does not accept poly(P) as phosphoryl donor Arabidopsis thaliana ?
-
 ?
2.7.1.23 NADH + poly(P)4
-
 Mycobacterium tuberculosis NADPH + poly(P)3
-
 ?
2.7.1.23 NADH + poly(P)4
-
 Methanocaldococcus jannaschii NADPH + poly(P)3
-
 ?
2.7.1.23 NADH + poly(P)4
-
 Pyrococcus horikoshii NADPH + poly(P)3
-
 ?
2.7.1.23 NADH + poly(P)4
-
 Bacillus subtilis NADPH + poly(P)3
-
 ?
2.7.1.23 NADH + poly(P)4
-
 Micrococcus luteus NADPH + poly(P)3
-
 ?
2.7.1.23 NADH + poly(P)4
-
 Mycobacterium tuberculosis H37Rv NADPH + poly(P)3
-
 ?
2.7.1.23 NADH + poly(P)4
-
 Methanocaldococcus jannaschii ATCC 43067 NADPH + poly(P)3
-
 ?
2.7.1.86 ATP + NAD+
-
 Saccharomyces cerevisiae ADP + NADP+
-
 ?
2.7.1.86 ATP + NADH isoform Pos5p shows 167% activity compared to NAD+ Saccharomyces cerevisiae ADP + NADPH
-
 ?
2.7.1.86 ATP + NADH isoform NADK1 shows 169% activity compared to NAD+ Arabidopsis thaliana ADP + NADPH
-
 ?
2.7.1.86 additional information does not accept poly(P) as phosphoryl donor Saccharomyces cerevisiae ?
-
 ?
3.1.3.11 D-fructose 1,6-bisphosphate + H2O
-
 Methanocaldococcus jannaschii D-fructose 6-phosphate + phosphate
-
 ?
3.1.3.11 D-fructose 1,6-bisphosphate + H2O
-
 Archaeoglobus fulgidus D-fructose 6-phosphate + phosphate
-
 ?
3.1.3.11 D-fructose 1,6-bisphosphate + H2O
-
 Methanocaldococcus jannaschii ATCC 43067 D-fructose 6-phosphate + phosphate
-
 ?
3.1.3.11 D-fructose 1,6-bisphosphate + H2O
-
 Archaeoglobus fulgidus ATCC 49558 D-fructose 6-phosphate + phosphate
-
 ?
3.1.3.108 NADP+ + H2O the enzyme regulates the intracellular balance of NAD(H) and NADP(H) Methanocaldococcus jannaschii NAD+ + phosphate
-
 ?
3.1.3.108 NADP+ + H2O the enzyme regulates the intracellular balance of NAD(H) and NADP(H) Archaeoglobus fulgidus NAD+ + phosphate
-
 ?
3.1.3.108 NADP+ + H2O
-
 Methanocaldococcus jannaschii NAD+ + phosphate
-
 ?
3.1.3.108 NADP+ + H2O
-
 Archaeoglobus fulgidus NAD+ + phosphate
-
 ?
3.1.3.108 NADP+ + H2O the enzyme regulates the intracellular balance of NAD(H) and NADP(H) Methanocaldococcus jannaschii ATCC 43067 NAD+ + phosphate
-
 ?
3.1.3.108 NADP+ + H2O
-
 Methanocaldococcus jannaschii ATCC 43067 NAD+ + phosphate
-
 ?
3.1.3.108 NADP+ + H2O the enzyme regulates the intracellular balance of NAD(H) and NADP(H) Archaeoglobus fulgidus ATCC 49558 NAD+ + phosphate
-
 ?
3.1.3.108 NADP+ + H2O
-
 Archaeoglobus fulgidus ATCC 49558 NAD+ + phosphate
-
 ?
3.1.3.108 NADPH + H2O the enzyme regulates the intracellular balance of NAD(H) and NADP(H) Methanocaldococcus jannaschii NADH + phosphate
-
 ?
3.1.3.108 NADPH + H2O the enzyme regulates the intracellular balance of NAD(H) and NADP(H) Archaeoglobus fulgidus NADH + phosphate
-
 ?
3.1.3.108 NADPH + H2O
-
 Methanocaldococcus jannaschii NADH + phosphate
-
 ?
3.1.3.108 NADPH + H2O
-
 Archaeoglobus fulgidus NADH + phosphate
-
 ?
3.1.3.108 NADPH + H2O the enzyme regulates the intracellular balance of NAD(H) and NADP(H) Methanocaldococcus jannaschii ATCC 43067 NADH + phosphate
-
 ?
3.1.3.108 NADPH + H2O
-
 Methanocaldococcus jannaschii ATCC 43067 NADH + phosphate
-
 ?
3.1.3.108 NADPH + H2O the enzyme regulates the intracellular balance of NAD(H) and NADP(H) Archaeoglobus fulgidus ATCC 49558 NADH + phosphate
-
 ?
3.1.3.108 NADPH + H2O
-
 Archaeoglobus fulgidus ATCC 49558 NADH + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.23 ? ? x 58000 Arabidopsis thaliana
2.7.1.23 ? ? x 110000 Arabidopsis thaliana
2.7.1.23 ? x * 46000 Saccharomyces cerevisiae
2.7.1.23 homodimer 2 * 30000 Bacillus subtilis
2.7.1.23 homodimer 2 * 32000 Sphingomonas sp. A1
2.7.1.23 homodimer 2 x 38000 Arabidopsis thaliana
2.7.1.23 homodimer 2 x 34000 Micrococcus luteus
2.7.1.23 homohexamer 6 x 30000 Escherichia coli
2.7.1.23 homohexamer 6 * 60000 Saccharomyces cerevisiae
2.7.1.23 homohexamer 6 * 60000, Utr1p Saccharomyces cerevisiae
2.7.1.23 homooctamer 8 * 60000 Saccharomyces cerevisiae
2.7.1.23 homooctamer 6 * 60000, Yef1p Saccharomyces cerevisiae
2.7.1.23 homotetramer 4 x 35000 Mycobacterium tuberculosis
2.7.1.23 homotetramer 4 x 49000 Homo sapiens
2.7.1.23 homotetramer 4 x 64000 Methanocaldococcus jannaschii
2.7.1.23 homotetramer 4 * 37000 Pyrococcus horikoshii
2.7.1.23 homotetramer 4 * 49000 Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.7.1.23 NadF
-
 Bacillus subtilis
2.7.1.23 NADK
-
 Sphingomonas sp. A1
2.7.1.23 NADK1
-
 Arabidopsis thaliana
2.7.1.23 NADK1
-
 Listeria monocytogenes
2.7.1.23 NADK2
-
 Arabidopsis thaliana
2.7.1.23 NADK2 isoform Arabidopsis thaliana
2.7.1.23 NADK3
-
 Arabidopsis thaliana
2.7.1.23 poly(P)/ATP-NAD kinase
-
 Mycobacterium tuberculosis
2.7.1.23 Pos5
-
 Saccharomyces cerevisiae
2.7.1.23 Ppnk
-
 Mycobacterium tuberculosis
2.7.1.23 styNadK
-
 Salmonella enterica
2.7.1.23 Utr1
-
 Saccharomyces cerevisiae
2.7.1.23 Utr1p
-
 Saccharomyces cerevisiae
2.7.1.23 Yef1p
-
 Saccharomyces cerevisiae
2.7.1.23 YfjB
-
 Escherichia coli
2.7.1.23 YjbN
-
 Bacillus subtilis
2.7.1.23 YtdI
-
 Bacillus subtilis
2.7.1.86 NADK1 isoform Arabidopsis thaliana
2.7.1.86 Pos5p
-
 Saccharomyces cerevisiae
3.1.3.11 AF2372
-
 Archaeoglobus fulgidus
3.1.3.11 MJ0109
-
 Methanocaldococcus jannaschii
3.1.3.11 MJ0917
-
 Methanocaldococcus jannaschii
3.1.3.108 AF2372
-
 Archaeoglobus fulgidus
3.1.3.108 MJ0109
-
 Methanocaldococcus jannaschii
3.1.3.108 MJ0917
-
 Methanocaldococcus jannaschii
3.1.3.108 NADP phosphatase
-
 Methanocaldococcus jannaschii
3.1.3.108 NADP phosphatase
-
 Archaeoglobus fulgidus
3.1.3.108 NADPase
-
 Methanocaldococcus jannaschii
3.1.3.108 NADPase
-
 Archaeoglobus fulgidus
3.1.3.108 NADPHase
-
 Methanocaldococcus jannaschii
3.1.3.108 NADPHase
-
 Archaeoglobus fulgidus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.23 212
-
 NADP+
-
 Methanocaldococcus jannaschii
2.7.1.23 212
-
 fructose-1,6-bisphosphate
-
 Methanocaldococcus jannaschii
2.7.1.23 236
-
 NADPH
-
 Methanocaldococcus jannaschii
3.1.3.11 8.4
-
 D-fructose 1,6-bisphosphate pH and temperature not specified in the publication Methanocaldococcus jannaschii
3.1.3.11 212
-
 D-fructose 1,6-bisphosphate pH and temperature not specified in the publication Methanocaldococcus jannaschii
3.1.3.11 212
-
 D-fructose 1,6-bisphosphate pH and temperature not specified in the publication Archaeoglobus fulgidus
3.1.3.108 5
-
 NADP+ pH and temperature not specified in the publication Methanocaldococcus jannaschii
3.1.3.108 6.9
-
 NADPH pH and temperature not specified in the publication Methanocaldococcus jannaschii
3.1.3.108 26.3
-
 NADP+ pH and temperature not specified in the publication Archaeoglobus fulgidus
3.1.3.108 40.3
-
 NADPH pH and temperature not specified in the publication Archaeoglobus fulgidus
3.1.3.108 212
-
 NADP+ pH and temperature not specified in the publication Methanocaldococcus jannaschii
3.1.3.108 236
-
 NADPH pH and temperature not specified in the publication Methanocaldococcus jannaschii

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.23 NAD+
-
 Homo sapiens
2.7.1.23 NAD+
-
 Saccharomyces cerevisiae
2.7.1.23 NAD+
-
 Arabidopsis thaliana
2.7.1.86 NADH
-
 Saccharomyces cerevisiae
2.7.1.86 NADH
-
 Arabidopsis thaliana

Expression

EC Number Organism Comment Expression
2.7.1.86 Saccharomyces cerevisiae transcription of POS5 is upregulated 3.4fold after treatment for 10-12 min with 2.5 mM Cu2+ up
2.7.1.86 Arabidopsis thaliana transcription of isoform NADK1 is upregulated by 3 and 8fold after treatment of plant cells with 5 mM H2O2 and irradiation, respectively up

General Information

EC Number General Information Comment Organism
2.7.1.23 physiological function chloroplast NAD kinase 2 has an important role in chlorophyll biosynthesis and protects chloroplasts from oxidative stress Arabidopsis thaliana
3.1.3.11 physiological function the enzyme regulates the intracellular balance of NAD(H) and NADP(H) Methanocaldococcus jannaschii
3.1.3.108 physiological function the enzyme regulates the intracellular balance of NAD(H) and NADP(H) Methanocaldococcus jannaschii
3.1.3.108 physiological function the enzyme regulates the intracellular balance of NAD(H) and NADP(H) Archaeoglobus fulgidus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.3.11 11.6
-
 D-fructose 1,6-bisphosphate pH and temperature not specified in the publication Methanocaldococcus jannaschii
3.1.3.11 56.8
-
 D-fructose 1,6-bisphosphate pH and temperature not specified in the publication Methanocaldococcus jannaschii
3.1.3.11 56.8
-
 D-fructose 1,6-bisphosphate pH and temperature not specified in the publication Archaeoglobus fulgidus
3.1.3.108 8.9
-
 NADPH pH and temperature not specified in the publication Methanocaldococcus jannaschii
3.1.3.108 9.2
-
 NADP+ pH and temperature not specified in the publication Methanocaldococcus jannaschii
3.1.3.108 56.8
-
 NADP+ pH and temperature not specified in the publication Methanocaldococcus jannaschii
3.1.3.108 63.1
-
 NADPH pH and temperature not specified in the publication Methanocaldococcus jannaschii
3.1.3.108 113
-
 NADPH pH and temperature not specified in the publication Archaeoglobus fulgidus
3.1.3.108 188
-
 NADP+ pH and temperature not specified in the publication Archaeoglobus fulgidus