Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Hayashi, Y.; Toyomasu, T.; Hirose, Y.; Onodera, Y.; Mitsuhashi, W.; Yamane, H.; Sassa, T.; Dairi, T.
    Comparison of the enzymatic properties of ent-copalyl diphosphate-synthases in the biosynthesis of phytoalexins and gibberellins in rice (2008), Biosci. Biotechnol. Biochem., 72, 523-530.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.5.1.13 expressed in Escherichia coli Oryza sativa

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.5.1.13 5-isopropyl-N,N,N,2-tetramethyl-4-[(1-piperidinylcarbonyl)oxy]anilinium chloride CPS1 activity exhibits approximately 70% inhibition by 0.1 mM Amo-1618, whereas the OsCPS2/OsCyc2 activity exhibits approximately 10% inhibition Oryza sativa
5.5.1.13 Co2+ 35-60% relative activity in the presence of 0.1 mM Co2+ compared to Mg2+ Oryza sativa
5.5.1.13 Fe2+ 5-10% relative activity in the presence of 0.1 mM Fe+ compared to Mg2+ Oryza sativa
5.5.1.13 geranylgeranyl diphosphate CPS1 but not CPS2/Cyc2 is inhibited by 0.05-0.06 mM geranylgeranyl diphosphate, CPS1 shows significant substrate inhibition in the presence of more than 0.05 mM geranylgeranyl diphosphate Oryza sativa
5.5.1.13 Mg2+ weak inhibition of CPS2/Cyc2 at 5mM Mg2+ Oryza sativa
5.5.1.13 Ni2+ 60-70% relative activity in the presence of 0.1 mM Ni2+ compared to Mg2+ Oryza sativa
5.5.1.13 Zn2+ CPS1 shows 30% enzymatic activity with 0.1 mM Zn2+ compared to Mg2+ Oryza sativa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.5.1.13 Mg2+ the enzyme activity is highest at 0.1mM of Mg2+ Oryza sativa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.5.1.13 80900
-
 CPS2/Cyc2, calculated from amino acid sequence Oryza sativa
5.5.1.13 84900
-
 CPS1, calculated from amino acid sequence Oryza sativa

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
5.5.1.13 2-mercaptoethanol 5 mM 2-mercaptoethanol is required for full enzymatic activity Oryza sativa
5.5.1.13 Glycerol 20% (v/v) glycerol is required for full enzymatic activity Oryza sativa
5.5.1.13 Tween 80 0.1% (v/v) Tween 80 is required for full enzymatic activity Oryza sativa

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.19 Oryza sativa
-
-
-
5.5.1.13 Oryza sativa
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.19 ent-copalyl diphosphate
-
 Oryza sativa ent-kaurene + diphosphate
-
 ?
5.5.1.13 copalyl diphosphate
-
 Oryza sativa ent-kaurene + diphosphate
-
 ?
5.5.1.13 geranylgeranyl diphosphate
-
 Oryza sativa ent-copalyl diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
5.5.1.13 monomer gel filtration Oryza sativa

Synonyms

EC Number Synonyms Comment Organism
4.2.3.19 KS1
-
 Oryza sativa
5.5.1.13 CPS1 isozyme Oryza sativa
5.5.1.13 CPS2/Cyc2 bifunctional enzyme, acts in the synthesis of oryzalexins A-F and phytocassanes A-E (phytoalexins) Oryza sativa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.5.1.13 30 40
-
 Oryza sativa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.5.1.13 30
-
 activity is fully retained after incubating at 30°C in 0.05 M Tris-HCl at pH 7.0 for 1 h Oryza sativa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5.1.13 7
-
-
 Oryza sativa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.5.1.13 6 8
-
 Oryza sativa