Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Yano, S.; Honda, A.; Rattanakit, N.; Noda, Y.; Wakayama, M.; Plikomol, A.; Tachiki, T.
    Cloning and expression of chitinase A gene from Streptomyces cyaneus SP-27: the enzyme participates in protoplast formation of Schizophyllum commune (2008), Biosci. Biotechnol. Biochem., 72, 1853-1859.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.14 chitinase A, DNA and amino acid sequence determination and analysis, expression in Escherichia coli Rosetta-gami B (DE 3), and coexpression of chitinase A with alpha-1,3-glucanase of Bacillus circulans KA-304 in Schizophyllum commune IFO 4928 leading to protoplast-forming activity from the mycelium in which chitinase A dominates alpha1,3-glucanase Streptomyces cyaneus
3.2.1.14 chitinase I, coexpression of chitinase I with alpha-1,3-glucanase of Bacillus circulans KA-304 in Schizophyllum commune IFO 4928 leading to protoplast-forming activity from the mycelium Niallia circulans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.14 28789
-
 x * 29000, ChiA, SDS-PAGE, x * 28789, sequence calculation Streptomyces cyaneus
3.2.1.14 29000
-
 x * 29000, ChiA, SDS-PAGE, x * 28789, sequence calculation Streptomyces cyaneus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Niallia circulans
-
-
-
3.2.1.14 Niallia circulans KA-304
-
-
-
3.2.1.14 Streptomyces cyaneus B3XZQ2
-
-
3.2.1.14 Streptomyces cyaneus SP-27 B3XZQ2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.14 recombinant chitinase A 46fold from Escherichia coli by anion exchange and hydrophobic interaction chromatography, followed by another anion exchange chromatographical step Streptomyces cyaneus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.14 25
-
 purified recombinant enzyme Streptomyces cyaneus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 chitin + H2O colloidal or powdered substrate Streptomyces cyaneus ?
-
 ?
3.2.1.14 chitin + H2O powdered substrate Niallia circulans ?
-
 ?
3.2.1.14 chitin + H2O colloidal or powdered substrate Streptomyces cyaneus SP-27 ?
-
 ?
3.2.1.14 chitin + H2O powdered substrate Niallia circulans KA-304 ?
-
 ?
3.2.1.14 ethylene glycol chitin + H2O preferred substrate Streptomyces cyaneus ?
-
 ?
3.2.1.14 ethylene glycol chitin + H2O preferred substrate Streptomyces cyaneus SP-27 ?
-
 ?
3.2.1.14 additional information no activity with 4-nitrophenyl-GlcNAc Streptomyces cyaneus ?
-
 ?
3.2.1.14 additional information no activity with 4-nitrophenyl-GlcNAc Streptomyces cyaneus SP-27 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.14 ? x * 29000, ChiA, SDS-PAGE, x * 28789, sequence calculation Streptomyces cyaneus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.14 chitinase A
-
 Streptomyces cyaneus
3.2.1.14 chitinase I
-
 Niallia circulans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.14 60
-
-
 Streptomyces cyaneus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.14 30 50
-
 Streptomyces cyaneus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.14 6.5
-
-
 Streptomyces cyaneus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.14 4.5 9
-
 Streptomyces cyaneus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.14 5.5 8.5
-
 Streptomyces cyaneus