EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.1.10 | 0.04 | - |
3-dehydroquinate | apparent Km, pDQD | Gluconobacter oxydans | |
4.2.1.10 | 0.05 | - |
3-dehydroquinate | apparent Km, sDQD | Gluconobacter oxydans |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
4.2.1.10 | cytoplasm | cDQD | Gluconobacter oxydans | 5737 | - |
4.2.1.10 | membrane | pDQD | Gluconobacter oxydans | 16020 | - |
4.2.1.10 | periplasm | pDQD | Gluconobacter oxydans | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.2.1.10 | 16000 | - |
pDQD, determined by SDS-PAGE, suggesting a multisubunit structure composed of 12 subunits | Gluconobacter oxydans |
4.2.1.10 | 16270 | - |
putative deduced molecular weight | Gluconobacter oxydans |
4.2.1.10 | 47000 | - |
sDQD, determined by SDS-PAGE, suggesting a multisubunit structure composed of eight subunits | Gluconobacter oxydans |
4.2.1.10 | 47270 | - |
putative deduced molecular weight | Gluconobacter oxydans |
4.2.1.10 | 189000 | - |
sDQD, determined by gel filtration | Gluconobacter oxydans |
4.2.1.10 | 191000 | - |
pDQD, determined by gel filtration | Gluconobacter oxydans |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.10 | 3-dehydroquinate | Gluconobacter oxydans | - |
3-dehydroshikimate + H2O | - |
? | |
4.2.1.10 | 3-dehydroquinate | Gluconobacter oxydans IFO 3244 | - |
3-dehydroshikimate + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.1.10 | Gluconobacter oxydans | - |
- |
- |
4.2.1.10 | Gluconobacter oxydans IFO 3244 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.2.1.10 | periplasmic and cytosolic 3-dehydroquinate dehydratases are purified using a DEAE-cellulose, a DEAE-Sephadex A-50, a Hydroxyapatite and a Sephadex G-200 column | Gluconobacter oxydans |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.2.1.10 | 0.0002 | - |
sDQD, cell-free extract | Gluconobacter oxydans |
4.2.1.10 | 0.0036 | - |
sDQD, purification step DEAE-cellulose | Gluconobacter oxydans |
4.2.1.10 | 0.02 | - |
pDQD, membrane fraction | Gluconobacter oxydans |
4.2.1.10 | 0.07 | - |
pDQD, solubilizez fraction | Gluconobacter oxydans |
4.2.1.10 | 0.09 | - |
pDQD, purification step DEAE-cellulose | Gluconobacter oxydans |
4.2.1.10 | 0.2 | - |
sDQD, purification step DEAE-Sephadex A-50 | Gluconobacter oxydans |
4.2.1.10 | 0.28 | - |
pDQD, purification step DEAE-Sephadex A-50 | Gluconobacter oxydans |
4.2.1.10 | 0.45 | - |
sDQD, purification step Sephadex G-200 | Gluconobacter oxydans |
4.2.1.10 | 0.83 | - |
sDQD, heat treatment | Gluconobacter oxydans |
4.2.1.10 | 0.97 | - |
pDQD, purification step Hydroxyapatite | Gluconobacter oxydans |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.10 | 3-dehydroquinate | - |
Gluconobacter oxydans | 3-dehydroshikimate + H2O | - |
? | |
4.2.1.10 | 3-dehydroquinate | - |
Gluconobacter oxydans IFO 3244 | 3-dehydroshikimate + H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.2.1.10 | multimer | - |
Gluconobacter oxydans |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.2.1.10 | 3-dehydroquinate dehydratase | - |
Gluconobacter oxydans |
4.2.1.10 | DQD | - |
Gluconobacter oxydans |
4.2.1.10 | pDQD | periplasmic 3-dehydroquinate dehydratase | Gluconobacter oxydans |
4.2.1.10 | sDQD | soluble, cytoplasmic 3-dehydroquinate dehydratase | Gluconobacter oxydans |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.1.10 | 25 | - |
activity assay | Gluconobacter oxydans |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.2.1.10 | 7.5 | 8 | activity assay | Gluconobacter oxydans |