EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.3 | 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide | complete inhibition at 50 mM | Aspergillus niger | |
3.1.1.3 | 2,4,6-Trinitrobenzenesulfonic acid | 55% residual activity at 2 mM | Aspergillus niger | |
3.1.1.3 | diethyldicarbonate | complete inhibition at 2 mM | Aspergillus niger | |
3.1.1.3 | additional information | not inhibited by citraconic anhydride (5 mM), N-ethylmaleimide (10 mM), iodoacetate (10 mM), N-acetylimidazole (10 mM), and phenylglyoxal (10 mM) | Aspergillus niger | |
3.1.1.3 | N-bromosuccinimide | complete inhibition at 0.001 mM | Aspergillus niger | |
3.1.1.3 | phenylmethylsulfonyl fluoride | 18% residual activity at 5 mM | Aspergillus niger |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.1.1.3 | extracellular | - |
Aspergillus niger | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.1.3 | 32000 | - |
SDS-PAGE | Aspergillus niger |
3.1.1.3 | 32200 | - |
1 * 32200, MALDI-TOF mass spectrometry | Aspergillus niger |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.1.3 | Aspergillus niger | - |
- |
- |
3.1.1.3 | Aspergillus niger NCIM 1207 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.1.3 | ammonium sulfate precipitation, phenyl Sepharose column chromatography, and Sephacryl-100 gel filtration | Aspergillus niger |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.3 | 4-nitrophenyl palmitate + H2O | - |
Aspergillus niger | 4-nitrophenol + palmitate | - |
? | |
3.1.1.3 | 4-nitrophenyl palmitate + H2O | - |
Aspergillus niger NCIM 1207 | 4-nitrophenol + palmitate | - |
? | |
3.1.1.3 | triolein + H2O | - |
Aspergillus niger | 1,2-diolein + oleate | - |
? | |
3.1.1.3 | triolein + H2O | - |
Aspergillus niger NCIM 1207 | 1,2-diolein + oleate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.1.3 | monomer | 1 * 32200, MALDI-TOF mass spectrometry | Aspergillus niger |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.1.3 | acidic lipase | - |
Aspergillus niger |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.3 | 50 | - |
- |
Aspergillus niger |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.3 | 40 | 70 | the activity decreases sharply above the optimum temperature with almost 95% loss of its original activity at 70°C, the lipase is stable at 40°C for 3 h, incubation at 50°C for 1 h results in 52% loss of lipase activity | Aspergillus niger |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.1.3 | 2.5 | - |
- |
Aspergillus niger |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
3.1.1.3 | Aspergillus niger | isoelectric focusing | - |
8.5 |