Literature summary extracted from

Basu, S.; Ghosh, A.; Bera, A.; Saha, M.N.; Chattopadhyay, D.; Chakrabarti, K.
Thermodynamic characterization of a highly thermoactive extracellular pectate lyase from a new isolate Bacillus pumilus DKS1 (2008), Biores. Technol., 99, 8088-8094.

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
4.2.2.2	EDTA	-	Bacillus pumilus
4.2.2.2	Ni2+	-	Bacillus pumilus
4.2.2.2	Zn2+	-	Bacillus pumilus

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
4.2.2.2	additional information	-	additional information	values of Km (polygalacturonic acid) is 0.44 mg/ml	Bacillus pumilus

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining
4.2.2.2	extracellular	-	Bacillus pumilus	-	-

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
4.2.2.2	Ca2+	enhances activity	Bacillus pumilus
4.2.2.2	Mg2+	slightly enhances enzyme activity	Bacillus pumilus
4.2.2.2	Mn2+	enhances activity	Bacillus pumilus

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
4.2.2.2	35000	-	SDS-PAGE	Bacillus pumilus

Organism

EC Number	Organism	UniProt	Comment	Textmining
4.2.2.2	Bacillus pumilus	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
4.2.2.2	an extracellular pectate lyase is purified from the culture filtrate of a newly isolated Bacillus pumilus DKS1 grown in pectin containing medium using ion-exchange and gel filtration chromatography	Bacillus pumilus

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4.2.2.2	polygalacturonic acid	-	Bacillus pumilus	?	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
4.2.2.2	pectate lyase	-	Bacillus pumilus

Temperature Optimum [°C]

EC Number	Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
4.2.2.2	75	-	-	Bacillus pumilus

Temperature Stability [°C]

EC Number	Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
4.2.2.2	70	-	beyond 70°C, enzyme activity is severely inhibited. The half-life is 6.93 min at 70°C as compared to 31 min at 40°C	Bacillus pumilus

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
4.2.2.2	8.5	-	-	Bacillus pumilus

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Release 2023.1 (January 2023)