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Literature summary extracted from

  • Tripathi, P.; Hofmann, H.; Kayastha, A.M.; Ulbrich-Hofmann, R.
    Conformational stability and integrity of alpha-amylase from mung beans: evidence of kinetic intermediate in GdmCl-induced unfolding (2008), Biophys. Chem., 137, 95-99.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.1 the circular dichroism spectrum shows two minima at 208 and 222 nm. Secondary structure analysis of the spectra estimated 31% alpha-helix, 15% beta-sheet, and 54% random coils Vigna radiata

General Stability

EC Number General Stability Organism
3.2.1.1 unfolding kinetics with guanidinium hydrochloride followed at concentrations in the range of 2.8-7.8 M by kinetic stopped-flow fluorescence measurements. At concentrations higher than 6 M, two unfolding phases are detected, whereas one phase is observed at concentrations lower than 5 M. These results suggest that the first reversible step of unfolding is slower than the second irreversible step at low guanidinium hydrochloride concentrations, whereas the rates of these two steps are opposite at high concentrations Vigna radiata

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.1 EDTA a distinct decrease of enzyme activity. The activity can not be regained after the addition of excess CaCl2, demonstrating the irreversibility of inactivation Vigna radiata

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.1 CaCl2 incubated in different concentrations 0.5-100 mM at 25°C overnight, with 10 mM EDTA, significant activation, with two apparent dissociation constants K1: 0.3 mM and K2: 5.4 mM Vigna radiata

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.1 Vigna radiata
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.1 from seeds Vigna radiata

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.1 seed
-
 Vigna radiata
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.1 starch + H2O pH 5.5, 55°C Vigna radiata ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.1 VrAmy
-
 Vigna radiata

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.1 55
-
 assay at Vigna radiata

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.1 48
-
 transition temperature T1/2 in the presence of 10 mM EDTA, thermal unfolding observed by circular dichroism spectrum measurements Vigna radiata
3.2.1.1 87
-
 transition temperature T1/2 in the presence of 5 mM CaCl2, thermal unfolding observed by circular dichroism spectrum measurements Vigna radiata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.1 5.5
-
 assay at Vigna radiata