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Literature summary extracted from

  • Ueno, K.; Araki, Y.; Hirai, N.; Saito, S.; Mizutani, M.; Sakata, K.; Todoroki, Y.
    Differences between the structural requirements for ABA 8'-hydroxylase inhibition and for ABA activity (2005), Bioorg. Med. Chem., 13, 3359-3370.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.14.137 (+-)-3'-methyl-abscisate competitive, 64% inhibition at 0.05 mM Arabidopsis thaliana
1.14.14.137 (+-)-abscisic acid-3'-thio-n-butyl thiol competitive, 51% inhibition at 0.05 mM Arabidopsis thaliana
1.14.14.137 1'-deoxy-(+)-S-abscisate competitive, 73% inhibition at 0.05 mM Arabidopsis thaliana
1.14.14.137 1'-deoxy-1'-fluoro-(+)-S-abscisate competitive, 100% inhibition at 0.05 mM Arabidopsis thaliana
1.14.14.137 2'alpha,3'alpha-dihydro-2'alpha,3'alpha-epoxy-(+)-S-abscisate competitive, 56% inhibition at 0.05 mM Arabidopsis thaliana
1.14.14.137 3'-azido-(+)-S-abscisate competitive, 38% inhibition at 0.05 mM Arabidopsis thaliana
1.14.14.137 3'-bromo-(+)-S-abscisate competitive, 65% inhibition at 0.05 mM Arabidopsis thaliana
1.14.14.137 3'-chloro-(+)-S-abscisate competitive, 70% inhibition at 0.05 mM Arabidopsis thaliana
1.14.14.137 3'-fluoro-(+)-S-abscisate competitive, 84% inhibition at 0.05 mM Arabidopsis thaliana
1.14.14.137 3'-iodo-(+)-S-abscisate competitive, 54% inhibition at 0.05 mM Arabidopsis thaliana
1.14.14.137 5'alpha,8'-cyclo-(+)-S-abscisate competitive, 28% inhibition at 0.05 mM Arabidopsis thaliana
1.14.14.137 6-nor-(+)-S-abscisate competitive, 88% inhibition at 0.05 mM Arabidopsis thaliana
1.14.14.137 7'-methyl-(+)-S-abscisate competitive, 83% inhibition at 0.05 mM Arabidopsis thaliana
1.14.14.137 7'-nor-(+)-S-abscisate competitive, 28% inhibition at 0.05 mM Arabidopsis thaliana
1.14.14.137 8',8',8'-trifluoro-(+)-S-abscisate competitive, 38% inhibition at 0.05 mM Arabidopsis thaliana
1.14.14.137 8',8'-difluoro-(+)-S-abscisate competitive, 83% inhibition at 0.05 mM Arabidopsis thaliana
1.14.14.137 8'-fluoro-(+)-S-abscisate competitive, 83% inhibition at 0.05 mM Arabidopsis thaliana
1.14.14.137 8'-methyl-(+)-S-abscisate competitive, 35% inhibition at 0.05 mM Arabidopsis thaliana
1.14.14.137 8'-methylene-(+)-S-abscisate competitive, 33% inhibition at 0.05 mM Arabidopsis thaliana
1.14.14.137 9',9',9'-trifluoro-(+)-S-abscisate competitive, 55% inhibition at 0.05 mM Arabidopsis thaliana
1.14.14.137 9',9'-difluoro-(+)-S-abscisate competitive, 76% inhibition at 0.05 mM Arabidopsis thaliana
1.14.14.137 9'-fluoro-(+)-S-abscisate competitive, 83% inhibition at 0.05 mM Arabidopsis thaliana
1.14.14.137 9'-methyl-(+)-S-abscisate competitive, 26% inhibition at 0.05 mM Arabidopsis thaliana
1.14.14.137 abscisic aldehyde competitive, 31% inhibition at 0.05 mM Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.137 Arabidopsis thaliana
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.137 (+)-S-abscisate + [reduced NADPH-hemoprotein reductase] + O2
-
 Arabidopsis thaliana 8'-hydroxyabscisate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.137 (+-)-3'-methyl-abscisate + [reduced NADPH-hemoprotein reductase] + O2 32% of the activity with (+)-S-abscisate Arabidopsis thaliana ? + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.137 (+-)-abscisic acid-3'-thio-n-butyl thiol + [reduced NADPH-hemoprotein reductase] + O2 2% of the activity with (+)-S-abscisate Arabidopsis thaliana ? + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.137 1'-deoxy-(+)-S-abscisate + [reduced NADPH-hemoprotein reductase] + O2 99% of the activity with (+)-S-abscisate Arabidopsis thaliana ? + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.137 1'-deoxy-1'-fluoro-(+)-S-abscisate + [reduced NADPH-hemoprotein reductase] + O2 94% of the activity with (+)-S-abscisate Arabidopsis thaliana ? + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.137 2'alpha,3'alpha-dihydro-2'alpha,3'alpha-epoxy-(+)-S-abscisate + [reduced NADPH-hemoprotein reductase] + O2 19% of the activity with (+)-S-abscisate Arabidopsis thaliana ? + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.137 3'-bromo-(+)-S-abscisate + [reduced NADPH-hemoprotein reductase] + O2 5% of the activity with (+)-S-abscisate Arabidopsis thaliana ? + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.137 3'-chloro-(+)-S-abscisate + [reduced NADPH-hemoprotein reductase] + O2 19% of the activity with (+)-S-abscisate Arabidopsis thaliana ? + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.137 3'-fluoro-(+)-S-abscisate + [reduced NADPH-hemoprotein reductase] + O2 68% of the activity with (+)-S-abscisate Arabidopsis thaliana ? + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.137 6-nor-(+)-S-abscisate + [reduced NADPH-hemoprotein reductase] + O2 60% of the activity with (+)-S-abscisate Arabidopsis thaliana ? + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.137 7'-methyl-(+)-S-abscisate + [reduced NADPH-hemoprotein reductase] + O2 15% of the activity with (+)-S-abscisate Arabidopsis thaliana ? + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.137 7'-nor-(+)-S-abscisate + [reduced NADPH-hemoprotein reductase] + O2 15% of the activity with (+)-S-abscisate Arabidopsis thaliana ? + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.137 8'-fluoro-(+)-S-abscisate + [reduced NADPH-hemoprotein reductase] + O2 11% of the activity with (+)-S-abscisate Arabidopsis thaliana ? + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.137 8'-methylene-(+)-S-abscisate + [reduced NADPH-hemoprotein reductase] + O2 4% of the activity with (+)-S-abscisate Arabidopsis thaliana ? + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.137 9',9'-difluoro-(+)-S-abscisate + [reduced NADPH-hemoprotein reductase] + O2 3% of the activity with (+)-S-abscisate Arabidopsis thaliana 9',9'-difluoro-8'-hydroxy-(+)-S-abscisate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.137 9'-fluoro-(+)-S-abscisate + [reduced NADPH-hemoprotein reductase] + O2 33% of the activity with (+)-S-abscisate Arabidopsis thaliana ? + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.137 9'-methyl-(+)-S-abscisate + [reduced NADPH-hemoprotein reductase] + O2 3% of the activity with (+)-S-abscisate Arabidopsis thaliana ? + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.137 additional information substrate recognition strictly requires the 6'-methyl groups Arabidopsis thaliana ?
-
 ?

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.14.14.137 0.00016
-
 6-nor-(+)-S-abscisate pH 7.25, 30°C Arabidopsis thaliana
1.14.14.137 0.00017
-
 8',8'-difluoro-(+)-S-abscisate pH 7.25, 30°C Arabidopsis thaliana
1.14.14.137 0.00025
-
 9',9'-difluoro-(+)-S-abscisate pH 7.25, 30°C Arabidopsis thaliana
1.14.14.137 0.00027
-
 8'-fluoro-(+)-S-abscisate pH 7.25, 30°C Arabidopsis thaliana
1.14.14.137 0.00071
-
 8',8',8'-trifluoro-(+)-S-abscisate pH 7.25, 30°C Arabidopsis thaliana
1.14.14.137 0.00078
-
 9'-fluoro-(+)-S-abscisate pH 7.25, 30°C Arabidopsis thaliana
1.14.14.137 0.00094
-
 8'-methyl-(+)-S-abscisate pH 7.25, 30°C Arabidopsis thaliana
1.14.14.137 0.00106
-
 9',9',9'-trifluoro-(+)-S-abscisate pH 7.25, 30°C Arabidopsis thaliana
1.14.14.137 0.00429
-
 9'-methyl-(+)-S-abscisate pH 7.25, 30°C Arabidopsis thaliana
1.14.14.137 0.00543
-
 8'-methylene-(+)-S-abscisate pH 7.25, 30°C Arabidopsis thaliana