EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.2.1 | hydroxylamine | inhibits acetate kinase reaction in a nonlinear and noncompetitive fashion, substantial inhibition at concentrations of 704 mM and minimal inhibition at concentrations of 250 microM hydroxylamine | Thermotoga maritima |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.2.1 | additional information | - |
additional information | lower Km of the Thermotoga maritima acetate kinase for acetate measured than that determined by earlier assay methods | Thermotoga maritima |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.2.1 | ATP + acetate | Thermotoga maritima | - |
ADP + acetyl phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.2.1 | Thermotoga maritima | Q9WYB1 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.2.1 | gel filtration | Thermotoga maritima |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.2.1 | additional information | - |
continuous assay, spectrophotometric quantification of phosphate achieved through measurement of the phosphorylysis of 2-amino-6-mercapto-7-methyl-purine riboside (MESG) to ribose-1-phosphate and 2-amino-6-mercapto-7-methyl-purine (MES) by purine nucleoside phosphorylase, sensitivity of the assay is in the range of 2-150 microM | Thermotoga maritima |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.2.1 | acetyl phosphate + hydroxylamine | high-energy acetyl phosphate reacts with hydroxylamine forming acetyl hydroxamate and inorganic phosphate | Thermotoga maritima | acetyl hydroxamate + phosphate | - |
? | |
2.7.2.1 | ATP + acetate | - |
Thermotoga maritima | ADP + acetyl phosphate | - |
? | |
2.7.2.1 | ATP + acetate | enzyme assay developed that can measure the released phosphate at a nanomolar level | Thermotoga maritima | ADP + acetyl phosphate | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.2.1 | 37 | - |
assay at | Thermotoga maritima |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.2.1 | 7.5 | - |
assay at | Thermotoga maritima |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.7.2.1 | 6.5 | 8.5 | assay can be performed over pH range of | Thermotoga maritima |