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Literature summary extracted from

  • Patel, M.P.; Baum, D.A.; Silverman, S.K.
    Improvement of DNA adenylation using T4 DNA ligase with a template strand and a strategically mismatched acceptor strand (2008), Bioorg. Chem., 36, 46-56.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.5.1.1 expressed in Escherichia coli BL21(DE3)pLysS cells Tequatrovirus T4

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.1 Tequatrovirus T4
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.5.1.1 ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m
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Tequatrovirus T4 AMP + diphosphate + (deoxyribonucleotide)m+n
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6.5.1.1 additional information T4 DNA can adenylate the 5'-phosphorylated donor DNA substrate with a DNA template, ATP, and an acceptor strand that has a strategically chosen C-T acceptor-template mismatch directly adjacent to the adenylation site, between 0.5 and 10 mM ATP the adenylation yield is high (about 60%), whereas the yield is lower outside of this range Tequatrovirus T4 ?
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?

Synonyms

EC Number Synonyms Comment Organism
6.5.1.1 T4 DNA ligase
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Tequatrovirus T4

Cofactor

EC Number Cofactor Comment Organism Structure
6.5.1.1 ATP
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Tequatrovirus T4