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Literature summary extracted from

  • Heggset, E.B.; Hoell, I.A.; Kristoffersen, M.; Eijsink, V.G.; Varum, K.M.
    Degradation of chitosans with chitinase G from Streptomyces coelicolor A3(2): production of chito-oligosaccharides and insight into subsite specificities (2009), Biomacromolecules, 10, 892-899.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.14 expression of ChiG in Escherichia coli Streptomyces coelicolor

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Streptomyces coelicolor
-
 11 putative family 18 chitinases and two putative family 19 chitinases, ChiF and ChiG
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.14 recombinant ChiG from Escherichia coli Streptomyces coelicolor

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.1.14 Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins mode of action of ChiG, family 19 chitinases use a single-displacement reaction with inversion of the configuration of the anomeric carbon, overview Streptomyces coelicolor
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.14 additional information
-
 substrate specificity, overview Streptomyces coelicolor

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 chitooligosaccharides + H2O degradation of a 64% acetylated chitosan yields a continuum of oligomers, showing that ChiG operates according to a nonprocessive, endo mode of action Streptomyces coelicolor ?
-
 ?
3.2.1.14 chitosan + H2O
-
 Streptomyces coelicolor ?
-
 ?
3.2.1.14 additional information substrate specificity, overview Streptomyces coelicolor ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.14 ChiG
-
 Streptomyces coelicolor
3.2.1.14 chitinase G
-
 Streptomyces coelicolor
3.2.1.14 additional information ChiG belongs to the bacterial family 19 of glycohydrolases Streptomyces coelicolor

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.14 37
-
 assay at Streptomyces coelicolor

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.14 5.5
-
 assay at Streptomyces coelicolor