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Literature summary extracted from

  • Dziedziejko, V.; Safranow, K.; Slowik-Zylka, D.; Machoy-Mokrzynska, A.; Millo, B.; Machoy, Z.; Chlubek, D.
    Characterisation of rat and human tissue alkaline phosphatase isoforms by high-performance liquid chromatography and agarose gel electrophoresis (2009), Biochimie, 91, 445-452.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.1 L-phenylalanine 10 mM, 100% inhibition; 10 mM, 100% inhibition; 10 mM, 37% inhibition; 10 mM, 37% inhibition, bone isoenzyme; 10 mM, 62% inhibition, isoenzyme rTI2A; 10 mM, 70% inhibition, isoenzyme rTI2B Rattus norvegicus
3.1.3.1 lectin 5 g/L, precipitation by lectin: 100%; 5 g/L, precipitation by lectin: 100%; 5 g/L, precipitation by lectin: 100%, bone isoenzyme; 5 g/L, precipitation by lectin: 58%, isoenzyme rTI2B; 5 g/L, precipitation by lectin: 76%, isoenzyme rTI2A Rattus norvegicus
3.1.3.1 Urea 3 mM, 100% inhibition; 3 mM, 100% inhibition; 3 mM, 68% inhibition, bone isoenzyme; 3 mM, 70% inhibition, isoenzyme rTI2B; 3 mM, 71% inhibition; 3 mM, 80% inhibition, isoenzyme rTI2A Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.1 membrane ALP is found in the soluble form as well as in the insoluble form bound to the mebrane via a GPI anchor Rattus norvegicus 16020
-
3.1.3.1 soluble ALP is found in the soluble form as well as in the insoluble form bound to the mebrane via a GPI anchor Rattus norvegicus
-
-

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.1 Rattus norvegicus
-
-
-
3.1.3.1 Rattus norvegicus P08289
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.1 by using HPLC separation of a intestinal homogenate two ALP peaks (rTI2A and rTI2B) are obtained Rattus norvegicus
3.1.3.1 by using HPLC separation of a rat bone homogenate one peak is obtained Rattus norvegicus
3.1.3.1 by using HPLC separation of a rat liver homogenate three peaks are obtained (rTL1, rTL2 and rTL3) Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.1 bone
-
 Rattus norvegicus
-
3.1.3.1 intestine
-
 Rattus norvegicus
-
3.1.3.1 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.1 additional information
-
 56% of intestinal activity is retained using HPLC for separation Rattus norvegicus
3.1.3.1 additional information
-
 57% of intestinal activity is retained using HPLC for separation. Increased activity is detected after cleaving ALP from the cellular membrane by GPI-specific phospholipase C Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.1 4-nitrophenyl phosphate + H2O
-
 Rattus norvegicus 4-nitrophenol + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.1 alkaline phosphatase
-
 Rattus norvegicus
3.1.3.1 ALP liver isoform Rattus norvegicus
3.1.3.1 ALP bone isoform Rattus norvegicus
3.1.3.1 ALP intestinal isoform Rattus norvegicus
3.1.3.1 rTB
-
 Rattus norvegicus
3.1.3.1 rTI2A
-
 Rattus norvegicus
3.1.3.1 rTI2B
-
 Rattus norvegicus
3.1.3.1 rTL1
-
 Rattus norvegicus
3.1.3.1 rTL2
-
 Rattus norvegicus
3.1.3.1 rTL3
-
 Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.1 56
-
 15 min, 100% inactivation Rattus norvegicus
3.1.3.1 56
-
 15 min, 27% inactivation, isoenzyme rTI2B Rattus norvegicus
3.1.3.1 56
-
 15 min, 32% inactivation Rattus norvegicus
3.1.3.1 56
-
 15 min, 52% inactivation, isoenzyme rTI2A Rattus norvegicus
3.1.3.1 56
-
 15 min, 78% inactivation, bone isoenzyme Rattus norvegicus