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Literature summary extracted from

  • Kusma, J.; Chaim, O.M.; Wille, A.C.; Ferrer, V.P.; Sade, Y.B.; Donatti, L.; Gremski, W.; Mangili, O.C.; Veiga, S.S.
    Nephrotoxicity caused by brown spider venom phospholipase-D (dermonecrotic toxin) depends on catalytic activity (2008), Biochimie, 90, 1722-1736.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.4.41 medicine a molecular mechanism for Loxosceles venom nephrotoxicity is demonstrated that is dependent on the catalytic activity of phospholipase-D toxin Loxosceles intermedia

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.41 expressed as a His-tagged fusion protein Loxosceles intermedia

Protein Variants

EC Number Protein Variants Comment Organism
3.1.4.41 H12A mice are exposed to recombinant wild-type phospholipase-D, or to an isoform with a H12A mutation in the catalytic domain resulting in no phospholipasic activity. Renal biopsies from mice treated with the wild-type toxin show glomerular edema, erythrocytes and collapse of Bowman’s space, edema and deposition of proteinaceous material within the tubular lumen. An absence of nephrotoxicity is shown in mice treated with the mutated toxin. Analyses of urine and blood show that wild type toxin induces hematuria and elevation of blood urea, while treatment with mutated toxin causes no changes. Mouse lethality experiments also show oliguria and mortality after treatment with wild-type toxin, but not following exposure to the mutated toxin. Wild-type toxin treatment causes cytoplasmic vacuolization, impaired spreading, reduction of cellular viability, and cell-cell and cell-substratum detachment in MDCK cells, while treatment with mutated isoform has no effect Loxosceles intermedia

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.41 Loxosceles intermedia P0CE81
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.41 using Ni-NTA chromatography Loxosceles intermedia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.41 sphingomyelin + H2O
-
 Loxosceles intermedia ceramide-1-phosphate + choline
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.4.41 phospholipase D
-
 Loxosceles intermedia
3.1.4.41 PLD
-
 Loxosceles intermedia

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.4.41 37
-
 assay at Loxosceles intermedia