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Literature summary extracted from

  • Lidome, E.; Graf, C.; Jaritz, M.; Schanzer, A.; Rovina, P.; Nikolay, R.; Bornancin, F.
    A conserved cysteine motif essential for ceramide kinase function (2008), Biochimie, 90, 1560-1565.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.138 expression in SF9 insect cell and transfection of FLAG-tagged or GFP-tagged wild type and mutant CerK proteins in COS-1 cell Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.138 C324A same specific acitity as wild-type CerK Homo sapiens
2.7.1.138 C347A lower specific acitity than wild-type CerK Homo sapiens
2.7.1.138 C351A lower specific acitity than wild-type CerK Homo sapiens
2.7.1.138 C354A lower specific acitity than wild-type CerK Homo sapiens
2.7.1.138 C398A same specific acitity as wild-type CerK Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.138 N-ethyl-maleimide thiol modifiying agent stops CerK acitivity, demonstrating that CerK contains exposed cysteine residues important for enzymatic activity Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.138 ceramide + ATP Homo sapiens
-
 ceramide 1-phosphate + ADP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.138 Homo sapiens Q8TCT0
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.138 ceramide + ATP
-
 Homo sapiens ceramide 1-phosphate + ADP
-
 ?
2.7.1.138 N-(6-((7-nitro-2-1,3-benzoxadiazol-4-yl)amino)hexanoyl)-D-erythro-sphingosine + ATP C6-ceramide Homo sapiens N-(6-((7-nitro-2-1,3-benzoxadiazol-4-yl)amino)hexanoyl)-D-erythro-sphingosine 1-phosphate + ADP
-
 ?
2.7.1.138 N-(7-(4-nitrobenzo-2-oxa-1,3-diazole))-6-aminocaproyl-D-erythro-sphingosine + ATP NBD-ceramide Homo sapiens N-(7-(4-nitrobenzo-2-oxa-1,3-diazole))-6-aminocaproyl-D-erythro-sphingosine 1-phosphate + ADP NBD-ceramide 1-phosphate ?
2.7.1.138 N-(8-((7-nitro-2-1,3-benzoxadiazol-4-yl)amino)octanoyl)-D-erythro-sphingosine + ATP C8-ceramide Homo sapiens N-(8-((7-nitro-2-1,3-benzoxadiazol-4-yl)amino)octanoyl)-D-erythro-sphingosine 1-phosphate + ADP
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.138 CERK
-
 Homo sapiens
2.7.1.138 hCERK
-
 Homo sapiens