Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Brito, C.; Kandzia, S.; Graca, T.; Conradt, H.S.; Costa, J.
    Human fucosyltransferase IX: specificity towards N-linked glycoproteins and relevance of the cytoplasmic domain in intra-Golgi localization (2008), Biochimie, 90, 1279-1290.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.152 FUT9, DNA and amino acid sequence determination and analysis, transient overexpression of His-tagged or V5-tagged wild-type and mutant FUT9 proteins in HeLa cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.152 additional information construction of mutants FUT9dcyt, FUT9d6, FUT9S/A, subcellular localization analysis of recombinant FUT9wt and mutants in HeLa cells Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.152 Golgi apparatus
-
Homo sapiens 5794
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.152 Mn2+ 1.9-fold increase in activity at Mn2+ concentrations between 2.5 and 10 mM Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.152 47000
-
x * 54000, glycosylated FUT9, SDS-PAGE, x * 47000, deglycosylated FUT9, SDS-PAGE Homo sapiens
2.4.1.152 54000
-
x * 54000, glycosylated FUT9, SDS-PAGE, x * 47000, deglycosylated FUT9, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.152 GDP-beta-L-fucose + asialo-erythropoietin Homo sapiens wild-type FUT9 efficiently fucosylates di-, tri- and tetraantennary N-glycans from asialoEPO ?
-
?
2.4.1.152 additional information Homo sapiens the alpha3-fucosyltransferase IX, FUT9, catalyses the transfer of fucose in an alpha3 linkage onto terminal type II Galbeta4GlcNAc acceptors, the final step in the biosynthesis of the Lewisx epitope, in neurons, overview ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.152 Homo sapiens Q5Q0U2
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.1.152 glycoprotein FUT9 is a type II membrane protein with three potential N-glycosylation sites Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.152 neuron
-
Homo sapiens
-
2.4.1.152 NT2-N cell NTERA-2/cl.D1 cells are cultured and differentiated into NT2N neurons Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.152 GDP-beta-L-fucose + asialo-erythropoietin wild-type FUT9 efficiently fucosylates di-, tri- and tetraantennary N-glycans from asialoEPO Homo sapiens ?
-
?
2.4.1.152 GDP-beta-L-fucose + GalNAcbeta1-4GlcNAcbeta1-3Galbeta1-4Glc activity of CEFT-2 Homo sapiens GDP + GalNAcbeta1-4(Fucalpha1-3)GlcNAcbeta1-3Galbeta1-4Glc
-
?
2.4.1.152 additional information the alpha3-fucosyltransferase IX, FUT9, catalyses the transfer of fucose in an alpha3 linkage onto terminal type II Galbeta4GlcNAc acceptors, the final step in the biosynthesis of the Lewisx epitope, in neurons, overview Homo sapiens ?
-
?

Subunits

EC Number Subunits Comment Organism
2.4.1.152 ? x * 54000, glycosylated FUT9, SDS-PAGE, x * 47000, deglycosylated FUT9, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.4.1.152 alpha3-fucosyltransferase IX
-
Homo sapiens
2.4.1.152 Fut9
-
Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.152 37
-
assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.152 7
-
-
Homo sapiens