EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.1.152 | FUT9, DNA and amino acid sequence determination and analysis, transient overexpression of His-tagged or V5-tagged wild-type and mutant FUT9 proteins in HeLa cells | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.4.1.152 | additional information | construction of mutants FUT9dcyt, FUT9d6, FUT9S/A, subcellular localization analysis of recombinant FUT9wt and mutants in HeLa cells | Homo sapiens |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.4.1.152 | Golgi apparatus | - |
Homo sapiens | 5794 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.152 | Mn2+ | 1.9-fold increase in activity at Mn2+ concentrations between 2.5 and 10 mM | Homo sapiens |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.4.1.152 | 47000 | - |
x * 54000, glycosylated FUT9, SDS-PAGE, x * 47000, deglycosylated FUT9, SDS-PAGE | Homo sapiens |
2.4.1.152 | 54000 | - |
x * 54000, glycosylated FUT9, SDS-PAGE, x * 47000, deglycosylated FUT9, SDS-PAGE | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.152 | GDP-beta-L-fucose + asialo-erythropoietin | Homo sapiens | wild-type FUT9 efficiently fucosylates di-, tri- and tetraantennary N-glycans from asialoEPO | ? | - |
? | |
2.4.1.152 | additional information | Homo sapiens | the alpha3-fucosyltransferase IX, FUT9, catalyses the transfer of fucose in an alpha3 linkage onto terminal type II Galbeta4GlcNAc acceptors, the final step in the biosynthesis of the Lewisx epitope, in neurons, overview | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.152 | Homo sapiens | Q5Q0U2 | - |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
2.4.1.152 | glycoprotein | FUT9 is a type II membrane protein with three potential N-glycosylation sites | Homo sapiens |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.4.1.152 | neuron | - |
Homo sapiens | - |
2.4.1.152 | NT2-N cell | NTERA-2/cl.D1 cells are cultured and differentiated into NT2N neurons | Homo sapiens | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.152 | GDP-beta-L-fucose + asialo-erythropoietin | wild-type FUT9 efficiently fucosylates di-, tri- and tetraantennary N-glycans from asialoEPO | Homo sapiens | ? | - |
? | |
2.4.1.152 | GDP-beta-L-fucose + GalNAcbeta1-4GlcNAcbeta1-3Galbeta1-4Glc | activity of CEFT-2 | Homo sapiens | GDP + GalNAcbeta1-4(Fucalpha1-3)GlcNAcbeta1-3Galbeta1-4Glc | - |
? | |
2.4.1.152 | additional information | the alpha3-fucosyltransferase IX, FUT9, catalyses the transfer of fucose in an alpha3 linkage onto terminal type II Galbeta4GlcNAc acceptors, the final step in the biosynthesis of the Lewisx epitope, in neurons, overview | Homo sapiens | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.4.1.152 | ? | x * 54000, glycosylated FUT9, SDS-PAGE, x * 47000, deglycosylated FUT9, SDS-PAGE | Homo sapiens |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.1.152 | alpha3-fucosyltransferase IX | - |
Homo sapiens |
2.4.1.152 | Fut9 | - |
Homo sapiens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.152 | 37 | - |
assay at | Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.152 | 7 | - |
- |
Homo sapiens |