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Literature summary extracted from
- 3-Hexulosephosphate synthase from Methylomonas aminofaciens 77a. Purification, properties and kinetics (1978), Biochim. Biophys. Acta, 523, 236-244.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.1.2.43 | Cu2+ | 45% inhibition with 1 mM Cu2+ | Methylomonas aminofaciens |  |
| 4.1.2.43 | EDTA | the enzyme is completely inhibited in the presence of EDTA, but the activity is restored depending on the amount of MgC12 supplemented | Methylomonas aminofaciens | |
| 4.1.2.43 | Glutaraldehyde | 22% inhibition at 4 mM | Methylomonas aminofaciens | |
| 4.1.2.43 | glyceraldehyde | 13% inhibition at 4 mM | Methylomonas aminofaciens | |
| 4.1.2.43 | glycolaldehyde | 49% inhibition at 4 mM | Methylomonas aminofaciens | |
| 4.1.2.43 | glyoxylate | 12% inhibition at 4 mM | Methylomonas aminofaciens | |
| 4.1.2.43 | Hg2+ | 81% inhibition with 1 mM Hg2+ | Methylomonas aminofaciens | |
| 4.1.2.43 | methylglyoxal | 26% inhibition at 4 mM | Methylomonas aminofaciens | |
| 4.1.2.43 | Pb2+ | 48% inhibition with 1 mM Pb2+ | Methylomonas aminofaciens | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.1.2.43 | 0.059 | - |
D-ribulose 5-phosphate | in 50 mM potassium phosphate buffer (pH 7.4), 5 mM MgCl2 | Methylomonas aminofaciens | |
| 4.1.2.43 | 0.29 | - |
formaldehyde | in 50 mM potassium phosphate buffer (pH 7.4), 5 mM MgCl2 | Methylomonas aminofaciens | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.1.2.43 | Cd2+ | the enzyme activity is also promoted with 1 mM Cd2+ | Methylomonas aminofaciens | |
| 4.1.2.43 | Co2+ | the enzyme activity is also promoted with 5 mM Co2+ | Methylomonas aminofaciens | |
| 4.1.2.43 | Fe2+ | the enzyme activity is also promoted with 1 mM Fe2+ | Methylomonas aminofaciens | |
| 4.1.2.43 | Mg2+ | essential requirement for Mg2+ (5 mM) | Methylomonas aminofaciens | |
| 4.1.2.43 | Mn2+ | essential requirement for Mn2+ (5 mM) | Methylomonas aminofaciens | |
| 4.1.2.43 | Zn2+ | the enzyme activity is also promoted with 5 mM Zn2+ | Methylomonas aminofaciens | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.1.2.43 | 23000 | - |
2 * 23000, SDS-PAGE | Methylomonas aminofaciens |
| 4.1.2.43 | 45000 | - |
native enzyme, gel filtration | Methylomonas aminofaciens |
| 4.1.2.43 | 47000 | - |
sedimentation velocity | Methylomonas aminofaciens |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.1.2.43 | Methylomonas aminofaciens | - |
- |
- |
| 4.1.2.43 | Methylomonas aminofaciens 77a | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.1.2.43 | DEAE-cellulose column chromatography, hydroxylapatite column chromatography, Sephadex G-150 gel filtration, Sephadex G-100 gel filtration, and DEAE-Sephadex A-50 gel filtration | Methylomonas aminofaciens |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.1.2.43 | 3.3 | - |
cell free extract | Methylomonas aminofaciens |
| 4.1.2.43 | 52 | - |
after 16fold purification | Methylomonas aminofaciens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.2.43 | D-ribulose 5-phosphate + formaldehyde | the enzyme is specific for formaldehyde and D-ribulose 5-phosphate | Methylomonas aminofaciens | D-arabino-hex-3-ulose 6-phosphate | - |
? | |
| 4.1.2.43 | D-ribulose 5-phosphate + formaldehyde | the enzyme is specific for formaldehyde and D-ribulose 5-phosphate | Methylomonas aminofaciens 77a | D-arabino-hex-3-ulose 6-phosphate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.1.2.43 | homodimer | 2 * 23000, SDS-PAGE | Methylomonas aminofaciens |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.1.2.43 | 3-hexulosephosphate synthase | - |
Methylomonas aminofaciens |
| 4.1.2.43 | D-arabino-3-hexulose 6-phosphate formaldehyde lyase | - |
Methylomonas aminofaciens |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.1.2.43 | 45 | - |
- |
Methylomonas aminofaciens |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.1.2.43 | 40 | 80 | almost full activity is retained after incubation at 40°C for 30 min, whereas at 80°C for 10 min the enzyme is completely inactivated | Methylomonas aminofaciens |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.1.2.43 | 8 | - |
- |
Methylomonas aminofaciens |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.1.2.43 | 7 | 8 | stable in neutral to slightly alkaline solutions | Methylomonas aminofaciens |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 4.1.2.43 | Methylomonas aminofaciens | isoelectric focusing | - |
5.1 |
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