Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Hai, T.; Lee, J.S.; Kim, T.J.; Suh, J.W.
    The role of the C-terminal region of cyanophycin synthetase from Nostoc ellipsosporum NE1 in its enzymatic activity and thermostability: A key function of Glu(856) (2008), Biochim. Biophys. Acta, 1794, 42-49.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.2.29 gene cphA, subcloning and expression of His-tagged wild-type and mutant enzymes in Escherichia coli strains DH5alpha and BL21(DE3) Nostoc ellipsosporum
6.3.2.30 gene cphA, subcloning and expression of His-tagged wild-type and mutant enzymes in Escherichia coli strains DH5alpha and BL21(DE3) Nostoc ellipsosporum

Protein Variants

EC Number Protein Variants Comment Organism
6.3.2.29 E856A site-directed mutagenesis, inactive mutant Nostoc ellipsosporum
6.3.2.29 E856V site-directed mutagenesis, inactive mutant Nostoc ellipsosporum
6.3.2.29 additional information construction of truncation mutant CphANE1?C45, which lacks up to 45 amino acids at its C-terminus, the mutant retains full enzymatic activity and produced polymers, however, the removal of one additional amino acid, Glu856, results in complete inactivation of CphANE1?C46, phenotype, overview. Removal of the sequence Leu867-Leu870 results in dramatically decreased thermostability Nostoc ellipsosporum
6.3.2.30 E856A site-directed mutagenesis, inactive mutant Nostoc ellipsosporum
6.3.2.30 E856V site-directed mutagenesis, inactive mutant Nostoc ellipsosporum
6.3.2.30 additional information construction of truncation mutant CphANE1-C45, which lacks up to 45 amino acids at its C-terminus, the mutant retains full enzymatic activity and produced polymers, however, the removal of one additional amino acid, Glu856, results in complete inactivation of CphANE1-C46, phenotype, overview. Removal of the sequence Leu867-Leu870 results in dramatically decreased thermostability Nostoc ellipsosporum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.2.29 Mg2+
-
 Nostoc ellipsosporum
6.3.2.30 Mg2+
-
 Nostoc ellipsosporum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.2.29 210000
-
 gel filtration, recombinant wild-type enzyme Nostoc ellipsosporum
6.3.2.30 210000
-
 gel filtration, recombinant wild-type enzyme Nostoc ellipsosporum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.2.29 ATP + [L-Asp(4-L-Arg)]n + L-Asp Nostoc ellipsosporum
-
 ADP + phosphate + [L-Asp(4-L-Arg)]n-L-Asp
-
 ?
6.3.2.29 ATP + [L-Asp(4-L-Arg)]n + L-Asp Nostoc ellipsosporum NE1
-
 ADP + phosphate + [L-Asp(4-L-Arg)]n-L-Asp
-
 ?
6.3.2.30 ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg Nostoc ellipsosporum
-
 ADP + phosphate + [L-Asp(4-L-Arg)]n+1
-
 ?
6.3.2.30 ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg Nostoc ellipsosporum NE1
-
 ADP + phosphate + [L-Asp(4-L-Arg)]n+1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.29 Nostoc ellipsosporum
-
-
-
6.3.2.29 Nostoc ellipsosporum NE1
-
-
-
6.3.2.30 Nostoc ellipsosporum
-
-
-
6.3.2.30 Nostoc ellipsosporum NE1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.29 recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Nostoc ellipsosporum
6.3.2.30 recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Nostoc ellipsosporum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.2.30 additional information
-
 activities of recombinant truncation mutant enzymes, overview Nostoc ellipsosporum
6.3.2.30 17.5
-
 purified recombinant wild-type enzyme at 50°C Nostoc ellipsosporum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.29 ATP + [L-Asp(4-L-Arg)]n + L-Asp
-
 Nostoc ellipsosporum ADP + phosphate + [L-Asp(4-L-Arg)]n-L-Asp
-
 ?
6.3.2.29 ATP + [L-Asp(4-L-Arg)]n + L-Asp role of the C-terminal region of CphANE1, Glu856 is critical for CphANE1 catalytic activity, overview Nostoc ellipsosporum ADP + phosphate + [L-Asp(4-L-Arg)]n-L-Asp
-
 ?
6.3.2.29 ATP + [L-Asp(4-L-Arg)]n + L-Asp
-
 Nostoc ellipsosporum NE1 ADP + phosphate + [L-Asp(4-L-Arg)]n-L-Asp
-
 ?
6.3.2.29 additional information the enzyme catalyzes the two-step reaction performing reactions of EC 6.3.2.30 and EC 6.3.2.29 Nostoc ellipsosporum ?
-
 ?
6.3.2.29 additional information the enzyme catalyzes the two-step reaction performing reactions of EC 6.3.2.30 and EC 6.3.2.29 Nostoc ellipsosporum NE1 ?
-
 ?
6.3.2.30 ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg
-
 Nostoc ellipsosporum ADP + phosphate + [L-Asp(4-L-Arg)]n+1
-
 ?
6.3.2.30 ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg role of the C-terminal region of CphANE1, Glu856 is critical for CphANE1 catalytic activity, overview Nostoc ellipsosporum ADP + phosphate + [L-Asp(4-L-Arg)]n+1
-
 ?
6.3.2.30 ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg
-
 Nostoc ellipsosporum NE1 ADP + phosphate + [L-Asp(4-L-Arg)]n+1
-
 ?
6.3.2.30 ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg role of the C-terminal region of CphANE1, Glu856 is critical for CphANE1 catalytic activity, overview Nostoc ellipsosporum NE1 ADP + phosphate + [L-Asp(4-L-Arg)]n+1
-
 ?
6.3.2.30 additional information the enzyme catalyzes the two-step reaction performing reactions of EC 6.3.2.30 and EC 6.3.2.29 Nostoc ellipsosporum ?
-
 ?
6.3.2.30 additional information the enzyme catalyzes the two-step reaction performing reactions of EC 6.3.2.30 and EC 6.3.2.29 Nostoc ellipsosporum NE1 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.2.29 dimer wild-type and truncation mutant CphANE1?C45 Nostoc ellipsosporum
6.3.2.30 dimer wild-type and truncation mutant CphANE1-C45 Nostoc ellipsosporum

Synonyms

EC Number Synonyms Comment Organism
6.3.2.29 CphA
-
 Nostoc ellipsosporum
6.3.2.29 cyanophycin synthetase
-
 Nostoc ellipsosporum
6.3.2.29 More cf. EC 6.3.2.30 Nostoc ellipsosporum
6.3.2.30 CphA
-
 Nostoc ellipsosporum
6.3.2.30 cyanophycin synthetase
-
 Nostoc ellipsosporum
6.3.2.30 More cf. EC 6.3.2.29 Nostoc ellipsosporum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.2.30 40
-
-
 Nostoc ellipsosporum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.3.2.29 additional information
-
 the predicted alpha-helical region that ranges from Val858 to Leu870 is important for the thermostability of the enzyme Nostoc ellipsosporum
6.3.2.30 additional information
-
 the predicted alpha-helical region that ranges from Val858 to Leu870 is important for the thermostability of the enzyme Nostoc ellipsosporum

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.2.29 ATP
-
 Nostoc ellipsosporum
6.3.2.30 ATP
-
 Nostoc ellipsosporum