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Literature summary extracted from

  • Gyemant, G.; Zajacz, A.; Becsi, B.; Ragunath, C.; Ramasubbu, N.; Erdodi, F.; Batta, G.; Kandra, L.
    Evidence for pentagalloyl glucose binding to human salivary alpha-amylase through aromatic amino acid residues (2009), Biochim. Biophys. Acta, 1794, 291-296.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.1 expression and purification carried out using a Bac-To-Bac Baculovirus Expression System Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.1 W58L reduced inhibitory efficiency of the mutants W58L and Y151M with 92 and 97% remaining enzyme activity at 0.00235 mM pentagalloyl glucose inhibitor concentration, respectively, pH 6.0, 37°C Homo sapiens
3.2.1.1 Y151M reduced inhibitory efficiency of the mutants W58L and Y151M with 92 and 97% remaining enzyme activity at 0.00235 mM pentagalloyl glucose inhibitor concentration, respectively, pH 6.0, 37°C Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.1 1,2,3,4,6-pentagalloyl-beta-D-glucose mixed non-competitive inhibition, KEI: 0.0026 mM, KEIS: 0.0039 mM, tested in a concentration range of 0.04 to 0.5 mM, reduced inhibitory efficiency of the mutants W58L and Y151M with 92 and 97% remaining enzyme activity at 0.00235 mM inhibitor concentration, respectively, pH 6.0, 37°C Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.1 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.1 recombinant protein, expression and purification carried out using a Bac-To-Bac Baculovirus Expression System Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.1 saliva type IXA Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.1 2-chloro-4-nitrophenyl alpha-D-galactopyranosyl-(1-4)-alpha-D-galactopyranosyl-(1-4)-alpha-D-galactopyranoside + H2O
-
 Homo sapiens 2-chloro-4-nitrophenol + alpha-D-galactopyranosyl-(1-4)-alpha-D-galactopyranosyl-(1-4)-alpha-D-galactopyranose the increase of absorbance of 2-chloro-4-nitrophenol liberated by HSA is measured continuously at 400 nm ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.1 HAS human salivary alpha-amylase Homo sapiens
3.2.1.1 human salivary alpha-amylase commercial preparation Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.1 6
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.1 6
-
 assay at Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.1 0.00235
-
 1,2,3,4,6-pentagalloyl-beta-D-glucose pH 6.0, 37°C, the mutants Y151M and W58L exhibit reduced inhibitory efficiency with 92 and 97% remaining enzyme activity at 0.00235 mM inhibitor concentration, respectively, pH 6.0, 37°C Homo sapiens