BRENDA - Enzyme Database

TLR-4 mediated group IVA phospholipase A2 activation is phosphatidic acid phosphohydrolase 1 and protein kinase C dependent

Grkovich, A.; Armando, A.; Quehenberger, O.; Dennis, E.A.; Biochim. Biophys. Acta 1791, 975-982 (2009)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.1.4
ATP
cellular GIVA PLA2 activity is induced by the treatment of cells with Ca+2 mobilizing agonists, such as the purinergic P2X7 receptor agonist ATP
Mus musculus
3.1.1.4
diacylglycerol
exogenous diacylglycerol stimulates GIVA PLA2-mediated arachidonic acid release
Mus musculus
3.1.1.4
additional information
involvement of phosphatidic acid phosphohydrolase 1 (PAP-1) and protein kinase C (PKC) in the Toll-like receptor-4 (TLR-4) activation of GIVA PLA2. Ca+2 influx is not required for TLR-4 mediated activation of the GIVA PLA2
Mus musculus
Engineering
EC Number
Amino acid exchange
Commentary
Organism
3.1.3.4
additional information
PAP-1 inhibition leads to the loss of cyclooxygenase 2 expression and PGE2 release
Mus musculus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.1.4
additional information
neither PAP-1 nor PKC inhibition reduces GIVA PLA2 synergistic activation by stimulation with Kdo2-Lipid A and ATP. Neither EDTA nor EGTA reduce the arachidonic acid release
Mus musculus
3.1.1.4
pyrrophenone
pretreatment of cells with pyrrophenone reduces Kdo2-lipid A stimulated arachidonic acid release to background levels
Mus musculus
3.1.3.4
bromoenol lactone
-
Mus musculus
3.1.3.4
additional information
PAP-1 chemical inhibition does not reduce the synergistic activation of group IVA phospholipase A2
Mus musculus
3.1.3.4
propranolol
-
Mus musculus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.3.4
a 3-sn-phosphatidate + H2O
Mus musculus
-
a 1,2-diacyl-sn-glycerol + phosphate
-
-
?
3.1.3.4
additional information
Mus musculus
phosphatidic acid phosphohydrolase 1 and protein kinase C are required for Toll-like receptor-4-mediated group IVA phospholipase A2 activation, regulation, overview
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.1.4
Mus musculus
-
-
-
3.1.3.4
Mus musculus
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.1.4
macrophage
-
Mus musculus
-
3.1.1.4
RAW-264.7 cell
-
Mus musculus
-
3.1.3.4
macrophage
-
Mus musculus
-
3.1.3.4
RAW-264.7 cell
-
Mus musculus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.4
glycerophospholipid + H2O
-
691109
Mus musculus
arachidonic acid + ?
-
-
-
?
3.1.3.4
a 3-sn-phosphatidate + H2O
-
691109
Mus musculus
a 1,2-diacyl-sn-glycerol + phosphate
-
-
-
?
3.1.3.4
additional information
phosphatidic acid phosphohydrolase 1 and protein kinase C are required for Toll-like receptor-4-mediated group IVA phospholipase A2 activation, regulation, overview
691109
Mus musculus
?
-
-
-
-
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.1.4
ATP
cellular GIVA PLA2 activity is induced by the treatment of cells with Ca+2 mobilizing agonists, such as the purinergic P2X7 receptor agonist ATP
Mus musculus
3.1.1.4
diacylglycerol
exogenous diacylglycerol stimulates GIVA PLA2-mediated arachidonic acid release
Mus musculus
3.1.1.4
additional information
involvement of phosphatidic acid phosphohydrolase 1 (PAP-1) and protein kinase C (PKC) in the Toll-like receptor-4 (TLR-4) activation of GIVA PLA2. Ca+2 influx is not required for TLR-4 mediated activation of the GIVA PLA2
Mus musculus
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
3.1.3.4
additional information
PAP-1 inhibition leads to the loss of cyclooxygenase 2 expression and PGE2 release
Mus musculus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.1.4
additional information
neither PAP-1 nor PKC inhibition reduces GIVA PLA2 synergistic activation by stimulation with Kdo2-Lipid A and ATP. Neither EDTA nor EGTA reduce the arachidonic acid release
Mus musculus
3.1.1.4
pyrrophenone
pretreatment of cells with pyrrophenone reduces Kdo2-lipid A stimulated arachidonic acid release to background levels
Mus musculus
3.1.3.4
bromoenol lactone
-
Mus musculus
3.1.3.4
additional information
PAP-1 chemical inhibition does not reduce the synergistic activation of group IVA phospholipase A2
Mus musculus
3.1.3.4
propranolol
-
Mus musculus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.3.4
a 3-sn-phosphatidate + H2O
Mus musculus
-
a 1,2-diacyl-sn-glycerol + phosphate
-
-
?
3.1.3.4
additional information
Mus musculus
phosphatidic acid phosphohydrolase 1 and protein kinase C are required for Toll-like receptor-4-mediated group IVA phospholipase A2 activation, regulation, overview
?
-
-
-
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.1.4
macrophage
-
Mus musculus
-
3.1.1.4
RAW-264.7 cell
-
Mus musculus
-
3.1.3.4
macrophage
-
Mus musculus
-
3.1.3.4
RAW-264.7 cell
-
Mus musculus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.4
glycerophospholipid + H2O
-
691109
Mus musculus
arachidonic acid + ?
-
-
-
?
3.1.3.4
a 3-sn-phosphatidate + H2O
-
691109
Mus musculus
a 1,2-diacyl-sn-glycerol + phosphate
-
-
-
?
3.1.3.4
additional information
phosphatidic acid phosphohydrolase 1 and protein kinase C are required for Toll-like receptor-4-mediated group IVA phospholipase A2 activation, regulation, overview
691109
Mus musculus
?
-
-
-
-