Literature summary extracted from
Theofilopoulos, S.; Lykidis, A.; Leondaritis, G.; Mangoura, D.
Novel function of the human presqualene diphosphate phosphatase as a type II phosphatidate phosphatase in phosphatidylcholine and triacylglyceride biosynthesis pathways (2008), Biochim. Biophys. Acta, 1781, 731-742.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.1.3.4 |
expression of presqualene diphosphate phosphatase in COS-7 cells, co-expression with CTP:phosphocholine cytidylyltransferase-alpha |
Homo sapiens |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
3.1.3.4 |
additional information |
presqualene diphosphate phosphatase, i.e. PA-PSP, overexpression accelerates the synthesis of phosphatidylcholine and causes accumulation of triacylglycerol with concomitant decrease in the rate of phosphatidylinositol synthesis. Co-expression of human CTP:phosphocholine cytidylyltransferase-alpha with PA-PSP enhances the effect of PA-PSP on phosphatidylcholine levels, yet attenuates its effect on triacylglycerol, overview |
Homo sapiens |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
3.1.3.4 |
additional information |
the presqualene diphosphate phosphatase is a potent Mg2+-independent, NEM-insensitive type II PAP |
Homo sapiens |
|
Localization
EC Number |
Localization |
Comment |
Organism |
GeneOntology No. |
Textmining |
---|
3.1.3.4 |
endoplasmic reticulum membrane |
the enzyme active site exposed to the cytosolic side of the membrane |
Homo sapiens |
5789 |
- |
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
3.1.3.4 |
additional information |
the presqualene diphosphate phosphatase is a potent Mg2+-independent, NEM-insensitive type II PAP |
Homo sapiens |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
3.1.3.4 |
32400 |
- |
x * 32400, recombinant PA-PSP, SDS-PAGE |
Homo sapiens |
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
3.1.3.4 |
a 3-sn-phosphatidate + H2O |
Homo sapiens |
phosphatidate phosphatases are key enzymes in lipid biosynthesis and signaling. Type I PAP enzymes participate in de-novo phospholipid biosynthesis, whereas type II PAP enzymes have an established role in lipid signaling. The eukaryotic, endoplasmic reticulum-resident PA-PSP is a bifunctional enzyme with specific type II PAP activity, and regulates, in addition to type I PAPs, the de-novo biosynthesis of phospholipids and triacylglycerols |
a 1,2-diacyl-sn-glycerol + phosphate |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.1.3.4 |
Homo sapiens |
- |
- |
- |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.1.3.4 |
a 3-sn-phosphatidate + H2O |
phosphatidate phosphatases are key enzymes in lipid biosynthesis and signaling. Type I PAP enzymes participate in de-novo phospholipid biosynthesis, whereas type II PAP enzymes have an established role in lipid signaling. The eukaryotic, endoplasmic reticulum-resident PA-PSP is a bifunctional enzyme with specific type II PAP activity, and regulates, in addition to type I PAPs, the de-novo biosynthesis of phospholipids and triacylglycerols |
Homo sapiens |
a 1,2-diacyl-sn-glycerol + phosphate |
- |
? |
|
3.1.3.4 |
a 3-sn-phosphatidate + H2O |
the presqualene diphosphate phosphatase is a potent Mg2+-independent, NEM-insensitive type II PAP |
Homo sapiens |
a 1,2-diacyl-sn-glycerol + phosphate |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
3.1.3.4 |
? |
x * 32400, recombinant PA-PSP, SDS-PAGE |
Homo sapiens |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.1.3.4 |
PA-PSP |
- |
Homo sapiens |
3.1.3.4 |
presqualene diphosphate phosphatase |
- |
Homo sapiens |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
3.1.3.4 |
37 |
- |
assay at |
Homo sapiens |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
3.1.3.4 |
6.5 |
- |
assay at |
Homo sapiens |