BRENDA - Enzyme Database

Novel function of the human presqualene diphosphate phosphatase as a type II phosphatidate phosphatase in phosphatidylcholine and triacylglyceride biosynthesis pathways

Theofilopoulos, S.; Lykidis, A.; Leondaritis, G.; Mangoura, D.; Biochim. Biophys. Acta 1781, 731-742 (2008)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
3.1.3.4
expression of presqualene diphosphate phosphatase in COS-7 cells, co-expression with CTP:phosphocholine cytidylyltransferase-alpha
Homo sapiens
Engineering
EC Number
Amino acid exchange
Commentary
Organism
3.1.3.4
additional information
presqualene diphosphate phosphatase, i.e. PA-PSP, overexpression accelerates the synthesis of phosphatidylcholine and causes accumulation of triacylglycerol with concomitant decrease in the rate of phosphatidylinositol synthesis. Co-expression of human CTP:phosphocholine cytidylyltransferase-alpha with PA-PSP enhances the effect of PA-PSP on phosphatidylcholine levels, yet attenuates its effect on triacylglycerol, overview
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.4
additional information
the presqualene diphosphate phosphatase is a potent Mg2+-independent, NEM-insensitive type II PAP
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.4
endoplasmic reticulum membrane
the enzyme active site exposed to the cytosolic side of the membrane
Homo sapiens
5789
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.3.4
additional information
the presqualene diphosphate phosphatase is a potent Mg2+-independent, NEM-insensitive type II PAP
Homo sapiens
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.3.4
32400
-
x * 32400, recombinant PA-PSP, SDS-PAGE
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.3.4
a 3-sn-phosphatidate + H2O
Homo sapiens
phosphatidate phosphatases are key enzymes in lipid biosynthesis and signaling. Type I PAP enzymes participate in de-novo phospholipid biosynthesis, whereas type II PAP enzymes have an established role in lipid signaling. The eukaryotic, endoplasmic reticulum-resident PA-PSP is a bifunctional enzyme with specific type II PAP activity, and regulates, in addition to type I PAPs, the de-novo biosynthesis of phospholipids and triacylglycerols
a 1,2-diacyl-sn-glycerol + phosphate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.3.4
Homo sapiens
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.4
a 3-sn-phosphatidate + H2O
phosphatidate phosphatases are key enzymes in lipid biosynthesis and signaling. Type I PAP enzymes participate in de-novo phospholipid biosynthesis, whereas type II PAP enzymes have an established role in lipid signaling. The eukaryotic, endoplasmic reticulum-resident PA-PSP is a bifunctional enzyme with specific type II PAP activity, and regulates, in addition to type I PAPs, the de-novo biosynthesis of phospholipids and triacylglycerols
691076
Homo sapiens
a 1,2-diacyl-sn-glycerol + phosphate
-
-
-
?
3.1.3.4
a 3-sn-phosphatidate + H2O
the presqualene diphosphate phosphatase is a potent Mg2+-independent, NEM-insensitive type II PAP
691076
Homo sapiens
a 1,2-diacyl-sn-glycerol + phosphate
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.1.3.4
?
x * 32400, recombinant PA-PSP, SDS-PAGE
Homo sapiens
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.3.4
37
-
assay at
Homo sapiens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.3.4
6.5
-
assay at
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.3.4
expression of presqualene diphosphate phosphatase in COS-7 cells, co-expression with CTP:phosphocholine cytidylyltransferase-alpha
Homo sapiens
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
3.1.3.4
additional information
presqualene diphosphate phosphatase, i.e. PA-PSP, overexpression accelerates the synthesis of phosphatidylcholine and causes accumulation of triacylglycerol with concomitant decrease in the rate of phosphatidylinositol synthesis. Co-expression of human CTP:phosphocholine cytidylyltransferase-alpha with PA-PSP enhances the effect of PA-PSP on phosphatidylcholine levels, yet attenuates its effect on triacylglycerol, overview
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.4
additional information
the presqualene diphosphate phosphatase is a potent Mg2+-independent, NEM-insensitive type II PAP
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.4
endoplasmic reticulum membrane
the enzyme active site exposed to the cytosolic side of the membrane
Homo sapiens
5789
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.3.4
additional information
the presqualene diphosphate phosphatase is a potent Mg2+-independent, NEM-insensitive type II PAP
Homo sapiens
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.3.4
32400
-
x * 32400, recombinant PA-PSP, SDS-PAGE
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.3.4
a 3-sn-phosphatidate + H2O
Homo sapiens
phosphatidate phosphatases are key enzymes in lipid biosynthesis and signaling. Type I PAP enzymes participate in de-novo phospholipid biosynthesis, whereas type II PAP enzymes have an established role in lipid signaling. The eukaryotic, endoplasmic reticulum-resident PA-PSP is a bifunctional enzyme with specific type II PAP activity, and regulates, in addition to type I PAPs, the de-novo biosynthesis of phospholipids and triacylglycerols
a 1,2-diacyl-sn-glycerol + phosphate
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.4
a 3-sn-phosphatidate + H2O
phosphatidate phosphatases are key enzymes in lipid biosynthesis and signaling. Type I PAP enzymes participate in de-novo phospholipid biosynthesis, whereas type II PAP enzymes have an established role in lipid signaling. The eukaryotic, endoplasmic reticulum-resident PA-PSP is a bifunctional enzyme with specific type II PAP activity, and regulates, in addition to type I PAPs, the de-novo biosynthesis of phospholipids and triacylglycerols
691076
Homo sapiens
a 1,2-diacyl-sn-glycerol + phosphate
-
-
-
?
3.1.3.4
a 3-sn-phosphatidate + H2O
the presqualene diphosphate phosphatase is a potent Mg2+-independent, NEM-insensitive type II PAP
691076
Homo sapiens
a 1,2-diacyl-sn-glycerol + phosphate
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.1.3.4
?
x * 32400, recombinant PA-PSP, SDS-PAGE
Homo sapiens
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.3.4
37
-
assay at
Homo sapiens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.3.4
6.5
-
assay at
Homo sapiens