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Literature summary extracted from

  • Yeang, C.; Varshney, S.; Wang, R.; Zhang, Y.; Ye, D.; Jiang, X.C.
    The domain responsible for sphingomyelin synthase (SMS) activity (2008), Biochim. Biophys. Acta, 1781, 610-617.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.7.8.27 drug development SMS is a target for drug design Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.8.27 transient expression of Flag-tagged wild-type SMS2 and mutants in HeLa cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.7.8.27 H285A site-directed mutagenesis, inactive mutant with wild-type localization pattern, overview. The point mutation only influences SMS2 catalytic activity but not the enzyme topology Homo sapiens
2.7.8.27 H328A site-directed mutagenesis, inactive mutant with wild-type localization pattern, overview. The point mutation only influences SMS2 catalytic activity but not the enzyme topology Homo sapiens
2.7.8.27 H332A site-directed mutagenesis, inactive mutant with wild-type localization pattern, overview. The point mutation only influences SMS2 catalytic activity but not the enzyme topology Homo sapiens
2.7.8.27 S273A site-directed mutagenesis, inactive mutant with wild-type localization pattern, overview. The point mutation only influences SMS2 catalytic activity but not the enzyme topology Homo sapiens
2.7.8.27 S283A site-directed mutagenesis, inactive mutant with wild-type localization pattern, overview. The point mutation only influences SMS2 catalytic activity but not the enzyme topology Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.8.27 Golgi apparatus isozyme SMS1 Homo sapiens 5794
-
2.7.8.27 Golgi apparatus isozyme SMS2 Homo sapiens 5794
-
2.7.8.27 plasma membrane isozyme SMS2 Homo sapiens 5886
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.8.27 additional information Homo sapiens sphingomyelin synthase sits at the crossroads of sphingomyelin, ceramide, and diacylglycerol metabolism. It utilizes ceramide and phosphatidylcholine as substrates to produce sphingomyelin and diacylglycerol, thereby regulating lipid messengers which play a role in cell survival and apoptosis ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.8.27 Homo sapiens Q86VZ5 SMS1; isozyme SMS1
-
2.7.8.27 Homo sapiens Q8NHU3 SMS2; isozyme SMS2
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.8.27 L-alpha-phosphatidylcholine + NBD-C6-ceramide i.e. N-(6-[(7-nitro-benz-2-oxa-1,3-diazo-4-yl)amino]caproyl)-ceramide Homo sapiens ?
-
 ?
2.7.8.27 additional information sphingomyelin synthase sits at the crossroads of sphingomyelin, ceramide, and diacylglycerol metabolism. It utilizes ceramide and phosphatidylcholine as substrates to produce sphingomyelin and diacylglycerol, thereby regulating lipid messengers which play a role in cell survival and apoptosis Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.8.27 More SMS isozyme domain structure and active site comformation, tertiary structure model by homology modeling, overview Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.7.8.27 SMS1
-
 Homo sapiens
2.7.8.27 SMS2
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.8.27 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.8.27 7.4
-
 assay at Homo sapiens