2.4.2.6
adenine + deoxycytidine
64.0% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxyadenosine + cytosine
r
2.4.2.6
adenine + deoxyguanosine
46.6% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxyadenosine + guanine
r
2.4.2.6
adenine + deoxyinosine
57.3% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxyadenosine + hypoxanthine
r
2.4.2.6
adenine + deoxyuridine
75.4% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxyadenosine + uracil
r
2.4.2.6
adenine + thymidine
74.6% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxyadenosine + thymine
r
2.4.2.6
cytosine + deoxyadenosine
27.7% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxycytidine + adenine
r
2.4.2.6
cytosine + deoxyguanosine
25.7% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxycytidine + guanine
r
2.4.2.6
cytosine + deoxyinosine
32.0% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxycytidine + hypoxanthine
r
2.4.2.6
cytosine + deoxyuridine
62.9% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxycytidine + uracil
r
2.4.2.6
cytosine + thymidine
53.5% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxycytidine + thymine
r
2.4.2.6
cytosine + thymidine
standard reaction for cofactor analyses as well as activating compound and inhibitor studies, in presence of 40 mM ammonium sulfate and 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxycytidine + thymine
r
2.4.2.6
hypoxanthine + deoxyadenosine
38.0% conversion after 4 h at standard reaction conditions: 2 mM substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxyinosine + adenine
r
2.4.2.6
hypoxanthine + deoxycytidine
51.9% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxyinosine + cytosine
r
2.4.2.6
hypoxanthine + deoxyguanosine
25.2% conversion after 4 h at standard reaction conditions: 2 mM substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxyinosine + guanine
r
2.4.2.6
hypoxanthine + deoxyuridine
65.6% conversion after 4 h at standard reaction conditions: 2 mM substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxyinosine + uracil
r
2.4.2.6
hypoxanthine + thymidine
76.8% conversion after 4 h at standard reaction conditions: 2 mM substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxyinosine + thymine
r
2.4.2.6
additional information
preferably purine 2-deoxyribonucleoside synthesis compared to pyrimidine deoxyribonucleoside synthesis although both are catalysed
691053
Lactococcus lactis
?
2.4.2.6
additional information
ribonucleoside, arabinosylnucleoside and 2,3-dideoxyribonucleoside are no substrates for base-exchange by YejD
691053
Lactococcus lactis
?
2.4.2.6
purine base + 2-deoxy-D-ribosyl-base
691053
Lactococcus lactis
purine deoxyribonucleoside + base
preferred over pyrimidine deoxyribonucleoside synthesis
r
2.4.2.6
pyrimidine base + 2-deoxy-D-ribosyl-base
691053
Lactococcus lactis
pyrimidine deoxyribonucleoside + base
r
2.4.2.6
thymine + deoxyadenosine
22.7% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
thymidine + adenine
r
2.4.2.6
thymine + deoxycytidine
38.3% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
thymidine + cytosine
r
2.4.2.6
thymine + deoxyguanosine
17.7% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
thymidine + guanine
r
2.4.2.6
thymine + deoxyinosine
19.5% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin, equilibrium reached after 2 h
691053
Lactococcus lactis
thymidine + hypoxanthine
r
2.4.2.6
thymine + deoxyuridine
53.7% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
thymidine + uracil
r
2.4.2.6
uracil + deoxyadenosine
17.1% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxyuridine + adenine
r
2.4.2.6
uracil + deoxycytidine
37.7% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxyuridine + cytosine
r
2.4.2.6
uracil + deoxyguanosine
16.1% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxyuridine + guanine
r
2.4.2.6
uracil + deoxyinosine
23.8% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxyuridine + hypoxanthine
r
2.4.2.6
uracil + thymidine
43.0% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxyuridine + thymine
r
2.4.2.6
adenine + deoxycytidine
64.0% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxyadenosine + cytosine
r
2.4.2.6
adenine + deoxyguanosine
46.6% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxyadenosine + guanine
r
2.4.2.6
adenine + deoxyinosine
57.3% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxyadenosine + hypoxanthine
r
2.4.2.6
adenine + deoxyuridine
75.4% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxyadenosine + uracil
r
2.4.2.6
adenine + thymidine
74.6% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxyadenosine + thymine
r
2.4.2.6
cytosine + deoxyadenosine
27.7% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxycytidine + adenine
r
2.4.2.6
cytosine + deoxyguanosine
25.7% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxycytidine + guanine
r
2.4.2.6
cytosine + deoxyinosine
32.0% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxycytidine + hypoxanthine
r
2.4.2.6
cytosine + deoxyuridine
62.9% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxycytidine + uracil
r
2.4.2.6
cytosine + thymidine
53.5% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxycytidine + thymine
r
2.4.2.6
cytosine + thymidine
standard reaction for cofactor analyses as well as activating compound and inhibitor studies, in presence of 40 mM ammonium sulfate and 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxycytidine + thymine
r
2.4.2.6
hypoxanthine + deoxyadenosine
38.0% conversion after 4 h at standard reaction conditions: 2 mM substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxyinosine + adenine
r
2.4.2.6
hypoxanthine + deoxycytidine
51.9% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxyinosine + cytosine
r
2.4.2.6
hypoxanthine + deoxyguanosine
25.2% conversion after 4 h at standard reaction conditions: 2 mM substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxyinosine + guanine
r
2.4.2.6
hypoxanthine + deoxyuridine
65.6% conversion after 4 h at standard reaction conditions: 2 mM substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxyinosine + uracil
r
2.4.2.6
hypoxanthine + thymidine
76.8% conversion after 4 h at standard reaction conditions: 2 mM substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxyinosine + thymine
r
2.4.2.6
additional information
preferably purine 2-deoxyribonucleoside synthesis compared to pyrimidine deoxyribonucleoside synthesis although both are catalysed
691053
Lactococcus lactis
?
2.4.2.6
additional information
ribonucleoside, arabinosylnucleoside and 2,3-dideoxyribonucleoside are no substrates for base-exchange by YejD
691053
Lactococcus lactis
?
2.4.2.6
purine base + 2-deoxy-D-ribosyl-base
691053
Lactococcus lactis
purine deoxyribonucleoside + base
preferred over pyrimidine deoxyribonucleoside synthesis
r
2.4.2.6
pyrimidine base + 2-deoxy-D-ribosyl-base
691053
Lactococcus lactis
pyrimidine deoxyribonucleoside + base
r
2.4.2.6
thymine + deoxyadenosine
22.7% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
thymidine + adenine
r
2.4.2.6
thymine + deoxycytidine
38.3% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
thymidine + cytosine
r
2.4.2.6
thymine + deoxyguanosine
17.7% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
thymidine + guanine
r
2.4.2.6
thymine + deoxyinosine
19.5% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin, equilibrium reached after 2 h
691053
Lactococcus lactis
thymidine + hypoxanthine
r
2.4.2.6
thymine + deoxyuridine
53.7% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
thymidine + uracil
r
2.4.2.6
uracil + deoxyadenosine
17.1% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxyuridine + adenine
r
2.4.2.6
uracil + deoxycytidine
37.7% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxyuridine + cytosine
r
2.4.2.6
uracil + deoxyguanosine
16.1% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxyuridine + guanine
r
2.4.2.6
uracil + deoxyinosine
23.8% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxyuridine + hypoxanthine
r
2.4.2.6
uracil + thymidine
43.0% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxyuridine + thymine
r