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Literature summary extracted from
- Mycobacterium tuberculosis RuvA induces two distinct types of structural distortions between the homologous and heterologous Holliday junctions (2009), Biochemistry, 48, 27-40.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.21.10 | expressed in Escherichia coli strain Origami (DE3)pLysS | Mycobacterium tuberculosis |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.1.21.10 | the tetrameric RuvA-Holliday junction complex II is stable in the presence of 750 mM NaCl, whereas the same amount of salt induces more than 80% dissociation of the octameric complex I | Mycobacterium tuberculosis |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.21.10 | Ca2+ | binding of two tetramers of RuvA on the junction is favored by divalent cations, Ca2+ enhances the binding of RuvA to the junction | Mycobacterium tuberculosis |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.21.10 | 22000 | - |
SDS-PAGE | Mycobacterium tuberculosis |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.21.10 | Mycobacterium tuberculosis | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.21.10 | DEAE-cellulose column chromatography, ammonium sulfate fractionation, Superdex-200 gel filtration, phosphocellulose column chromatography, and hydroxyapatite Bio-Gel HTP column chromatography | Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.21.10 | Holliday junction in DNA + H2O | RuvA protein binds Holliday junctions in preference to any other substrate | Mycobacterium tuberculosis | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.21.10 | RuvA | RuvA is a Holliday junction-specific DNA-binding protein and facilitates the interaction of RuvB with the junction | Mycobacterium tuberculosis |
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