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Literature summary extracted from

  • Robertson, W.D.; Warncke, K.
    Photolysis of adenosylcobalamin and radical pair recombination in ethanolamine ammonia-lyase probed on the micro- to millisecond time scale by using time-resolved optical absorption spectroscopy (2009), Biochemistry, 48, 140-147.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.3.1.7 Escherichia coli overexpression strain incorporating the cloned Salmonella typhimurium EAL coding sequence Salmonella enterica subsp. enterica serovar Typhimurium

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.3.1.7 (S)-1-amino-2-propanol inactive substrate analogue Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.1.7 additional information
-
 additional information a dissociation constant for coenzyme B12 binding to EAL of 0.0005 mM is estimated from the Michaelis constant, which indicates that the free cofactor represents 4% of the total cofactor in the sample Salmonella enterica subsp. enterica serovar Typhimurium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.3.1.7 Co2+ substrate binding is required for cobalt-carbon bond cleavage in the native system. Salmonella enterica subsp. enterica serovar Typhimurium

Organism

EC Number Organism UniProt Comment Textmining
4.3.1.7 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.1.7
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.3.1.7 additional information
-
 specific activity of the purified enzyme with aminoethanol as the substrate is 35-45 micromol/min/mg, determined by using the coupled assay with alcohol dehydrogenase/NADH Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.1.7 (S)-1-amino-2-propanol inactive substrate analogue, which binds to the substrate binding site in EAL but does not form the cob(II)alamin-substrate radical pair state Salmonella enterica subsp. enterica serovar Typhimurium propanal + NH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.3.1.7 EAL
-
 Salmonella enterica subsp. enterica serovar Typhimurium
4.3.1.7 ethanolamine ammonia-lyase
-
 Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3.1.7 7.5
-
 assay at Salmonella enterica subsp. enterica serovar Typhimurium

Cofactor

EC Number Cofactor Comment Organism Structure
4.3.1.7 coenzyme B12 adenosylcobalamin Salmonella enterica subsp. enterica serovar Typhimurium

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.3.1.7 0.039
-
 (S)-1-amino-2-propanol dissociation constant for interaction with holo-EAL Salmonella enterica subsp. enterica serovar Typhimurium