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Literature summary extracted from

  • Eydoux, C.; Spinelli, S.; Davis, T.L.; Walker, J.R.; Seitova, A.; Dhe-Paganon, S.; De Caro, A.; Cambillau, C.; Carriere, F.
    Structure of human pancreatic lipase-related protein 2 with the lid in an open conformation (2008), Biochemistry, 47, 9553-9564.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.26 additional information using the partly soluble short chain tributyrin, the activity of both HPLRP2 N336Q mutant and HPLRP2 is high in the presence of bile salts and in the absence of colipase Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.26 expressed in SF9 insect cells (wild type enzyme) and in Pichia pastoris (mutant enzyme N336Q) Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.1.26 hanging drop vapour diffusion method, using 1.17 M (NH4)2SO4, 0.1 M Na-cacodylate pH 5.5, 0.04 M NaCl for wild type enzyme, and 2.05 M ammonium sulfate, 0.1 M HEPES, pH 6.7 for mutant enzyme N336Q Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.26 N336Q the mutant enzyme is not glycosylated and shows identical kinetic properties to the wild type enzyme Homo sapiens
3.1.1.32 N336Q the mutant shows a significant phospholipase A1 activity on mixed phospholipid-bile salt micelles Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.26 E600 in the absence of bile salts and colipase, the time required to obtain 50% inactivation of PLRP2 by E600 is 3 min, in the presence of 4 mM sodium taurodeoxycholate and colipase, the rate of inhibition of PLRP2 by E600 increases significantly and 50% inhibition is reached within 1 min Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.26 50080
-
mature PLRP2, calculated from amino acid sequence Homo sapiens
3.1.1.26 52820
-
glycosylated enzyme, LC-ESI-TOF mass spectrometry Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.26 additional information Homo sapiens acts preferentially on substrates forming monomers or small aggregates (micelles) dispersed in solution like monoglycerides, phospholipids and galactolipids ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.26 Homo sapiens P54317
-
-
3.1.1.32 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.1.26 glycoprotein
-
Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.26 HiTrap Q HP column chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.26 pancreas
-
Homo sapiens
-
3.1.1.32 pancreas
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.26 1.3
-
wild type enzyme, using 1,2(2,3)-diolein as substrate, in 1 mM Tris-HCl, at pH 8.0, at 37°C, in the absence of colipase Homo sapiens
3.1.1.26 3.4
-
mutant enzyme N336Q, using 1,2(2,3)-diolein as substrate, in 1 mM Tris-HCl, at pH 8.0, at 37°C, in the absence of colipase Homo sapiens
3.1.1.26 4.3
-
mutant enzyme N336Q, using triolein as substrate, in 1 mM Tris-HCl, at pH 8.0, at 37°C, in the absence of colipase Homo sapiens
3.1.1.26 5
-
wild type enzyme, using triolein as substrate, in 1 mM Tris-HCl, at pH 8.0, at 37°C, in the absence of colipase Homo sapiens
3.1.1.26 40.2
-
mutant enzyme N336Q, using trioctanoin as substrate, in 1 mM Tris-HCl, at pH 8.0, at 37°C, in the absence of colipase Homo sapiens
3.1.1.26 43
-
wild type enzyme, using trioctanoin as substrate, in 1 mM Tris-HCl, at pH 8.0, at 37°C, in the absence of colipase Homo sapiens
3.1.1.26 212
-
mutant enzyme N336Q, using tributyrin as substrate, in 1 mM Tris-HCl, at pH 8.0, at 37°C, in the absence of colipase Homo sapiens
3.1.1.26 247
-
mutant enzyme N336Q, using 1(3)-monoolein as substrate, in 1 mM Tris-HCl, at pH 8.0, at 37°C, in the absence of colipase Homo sapiens
3.1.1.26 287
-
wild type enzyme, using tributyrin as substrate, in 1 mM Tris-HCl, at pH 8.0, at 37°C, in the absence of colipase Homo sapiens
3.1.1.26 317
-
wild type enzyme, using 1(3)-monoolein as substrate, in 1 mM Tris-HCl, at pH 8.0, at 37°C, in the absence of colipase Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.26 1(3)-monoolein + H2O
-
Homo sapiens ?
-
?
3.1.1.26 1,2(2,3)-diolein + H2O
-
Homo sapiens ?
-
?
3.1.1.26 1,2-di-O-dodecanoyl-3-O-beta-D-galactopyranosyl-sn-glycerol + H2O galactolipase activity of LRP2 Homo sapiens 2,3-dihydroxypropyl beta-D-galactopyranoside + dodecanoate
-
?
3.1.1.26 3-O-(6-O-alpha-D-galactopyranosyl-beta-D-galactopyranosyl)-1,2-di-O-dodecanoyl-sn-glycerol + H2O galactolipase activity of LRP2 Homo sapiens 2,3-dihydroxypropyl 6-O-beta-D-galactopyranosyl-beta-D-galactopyranoside + 2 dodecanoate
-
?
3.1.1.26 digalactosyldiacylglycerol + H2O
-
Homo sapiens digalactosylglycerol + fatty acid
-
?
3.1.1.26 L-alpha-phosphatidylcholine + H2O phospholipase activity of LRP2 Homo sapiens ?
-
?
3.1.1.26 monogalactosyldiacylglycerol + H2O
-
Homo sapiens galactosylglycerol + fatty acid
-
?
3.1.1.26 additional information acts preferentially on substrates forming monomers or small aggregates (micelles) dispersed in solution like monoglycerides, phospholipids and galactolipids Homo sapiens ?
-
?
3.1.1.26 tributyrin + H2O
-
Homo sapiens ?
-
?
3.1.1.26 trioctanoin + H2O
-
Homo sapiens ?
-
?
3.1.1.26 triolein + H2O
-
Homo sapiens ?
-
?
3.1.1.32 phosphatidylcholine + H2O
-
Homo sapiens 2-acylglycerophosphocholine + a fatty acid anion
-
?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.26 pancreatic lipase-related protein 2
-
Homo sapiens
3.1.1.26 PLRP2
-
Homo sapiens
3.1.1.32 pancreatic lipase-associated protein 2
-
Homo sapiens
3.1.1.32 PLRP2 PLRP2 shows lipase, phospholipase A1 and galactolipase activities Homo sapiens