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Literature summary extracted from

  • Taylor, A.M.; Farrar, C.E.; Jarrett, J.T.
    9-Mercaptodethiobiotin is formed as a competent catalytic intermediate by Escherichia coli biotin synthase (2008), Biochemistry, 47, 9309-9317.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.1.6
-
Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.8.1.6 N153S inactive for biotin production, enzyme can bind AdoMet and DTB and can convert DTB to 9-mercaptodethiobiotin, the majority of 9- mercaptodethiobiotin is released into the buffer and not bound to the enzyme, suggesting that dissociation of 9- mercaptodethiobiotin is faster than the second half reaction for biotin formation Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.1.6 0.002
-
dethiobiotin
-
Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.1.6 dethiobiotin + sulfur + 2 S-adenosyl-L-methionine Escherichia coli 9-mercaptodethiobiotin is formed as a competent catalytic intermediate by Escherichia coli biotin synthase biotin + 2 L-methionine + 2 5'-deoxyadenosine
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.8.1.6 Escherichia coli
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.8.1.6 dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin mechanism Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.1.6 dethiobiotin + sulfur + 2 S-adenosyl-L-methionine 9-mercaptodethiobiotin is formed as a competent catalytic intermediate by Escherichia coli biotin synthase Escherichia coli biotin + 2 L-methionine + 2 5'-deoxyadenosine
-
?

Synonyms

EC Number Synonyms Comment Organism
2.8.1.6 BioB
-
Escherichia coli
2.8.1.6 biotin synthase
-
Escherichia coli