EC Number | Cloned (Comment) | Organism |
---|---|---|
2.8.1.6 | - |
Escherichia coli |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.8.1.6 | N153S | inactive for biotin production, enzyme can bind AdoMet and DTB and can convert DTB to 9-mercaptodethiobiotin, the majority of 9- mercaptodethiobiotin is released into the buffer and not bound to the enzyme, suggesting that dissociation of 9- mercaptodethiobiotin is faster than the second half reaction for biotin formation | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.8.1.6 | 0.002 | - |
dethiobiotin | - |
Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.8.1.6 | dethiobiotin + sulfur + 2 S-adenosyl-L-methionine | Escherichia coli | 9-mercaptodethiobiotin is formed as a competent catalytic intermediate by Escherichia coli biotin synthase | biotin + 2 L-methionine + 2 5'-deoxyadenosine | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.8.1.6 | Escherichia coli | - |
- |
- |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.8.1.6 | dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin | mechanism | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.8.1.6 | dethiobiotin + sulfur + 2 S-adenosyl-L-methionine | 9-mercaptodethiobiotin is formed as a competent catalytic intermediate by Escherichia coli biotin synthase | Escherichia coli | biotin + 2 L-methionine + 2 5'-deoxyadenosine | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.8.1.6 | BioB | - |
Escherichia coli |
2.8.1.6 | biotin synthase | - |
Escherichia coli |