BRENDA - Enzyme Database show

Systematic exploration of active site mutations on human deoxycytidine kinase substrate specificity

Iyidogan, P.; Lutz, S.; Biochemistry 47, 4711-4720 (2008)

Data extracted from this reference:

Engineering
EC Number
Amino acid exchange
Commentary
Organism
2.7.1.74
A100V/R104M/D133A
12fold increase in ratio kcat/Km for deoxycytidine
Homo sapiens
2.7.1.74
A100V/R104M/D133S
6fold increase in ratio kcat/Km for deoxycytidine
Homo sapiens
2.7.1.74
A100V/R104M/D133T
ratio kcat/Km for deoxycytidine similar to wild-type
Homo sapiens
2.7.1.74
D47E/R104Q/D133G/N163I/F242L
1.5fold increase in ratio kcat/Km for deoxycytidine
Homo sapiens
2.7.1.74
additional information
R104 and D133 are key residues for substrate specificity
Homo sapiens
2.7.1.74
R104M/D133A
4fold increase in ratio kcat/Km for deoxycytidine
Homo sapiens
2.7.1.74
R104M/D133N
3fold increase in ratio kcat/Km for deoxycytidine
Homo sapiens
2.7.1.74
R104M/D133S
6fold increase in ratio kcat/Km for deoxycytidine
Homo sapiens
2.7.1.74
R104M/D133T
mutant with reversed substrate specificity, with elevated specific constant for thymidine phosphorylation and decreased activity for deeoxycytidine, deoxyadenosine, and deoxyguanosine
Homo sapiens
2.7.1.74
R104Q/D133G
mutant is a generalist kinase with broader specificity and elevated turnover compared with wild-type
Homo sapiens
2.7.1.74
R104Q/D133N
1.5fold increase in ratio kcat/Km for deoxycytidine
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.74
0.001
-
deoxycytidine
wild-type, pH 7.5, 30°C; wild-type, pH 7.5, 37°C
Homo sapiens
2.7.1.74
0.00104
-
deoxycytidine
mutant R104Q/D133G, pH 7.5, 30°C
Homo sapiens
2.7.1.74
0.00141
-
deoxycytidine
mutant D47E/R104Q/D133G/N163I/F242L, pH 7.5, 30°C
Homo sapiens
2.7.1.74
0.00154
-
deoxycytidine
mutant A100V/R104M/D133A, pH 7.5, 37°C
Homo sapiens
2.7.1.74
0.002
-
deoxycytidine
mutant R104M/D133A, pH 7.5, 37°C
Homo sapiens
2.7.1.74
0.00388
-
thymidine
mutant A100V/R104M/D133T, pH 7.5, 37°C
Homo sapiens
2.7.1.74
0.00455
-
deoxycytidine
mutant R104M/D133S, pH 7.5, 37°C
Homo sapiens
2.7.1.74
0.00525
-
deoxycytidine
mutant A100V/R104M/D133S, pH 7.5, 37°C
Homo sapiens
2.7.1.74
0.00558
-
thymidine
mutant A100V/R104M/D133S, pH 7.5, 37°C
Homo sapiens
2.7.1.74
0.0058
-
thymidine
mutant R104M/D133T, pH 7.5, 37°C
Homo sapiens
2.7.1.74
0.0086
-
deoxycytidine
mutant R104Q/D133N, pH 7.5, 30°C
Homo sapiens
2.7.1.74
0.0118
-
deoxycytidine
mutant R104M/D133N, pH 7.5, 37°C
Homo sapiens
2.7.1.74
0.0189
-
thymidine
mutant R104M/D133S, pH 7.5, 37°C
Homo sapiens
2.7.1.74
0.0209
-
deoxycytidine
mutant R104M/D133T, pH 7.5, 37°C
Homo sapiens
2.7.1.74
0.025
-
thymidine
mutant D47E/R104Q/D133G/N163I/F242L, pH 7.5, 30°C
Homo sapiens
2.7.1.74
0.0256
-
thymidine
mutant R104M/D133N, pH 7.5, 37°C
Homo sapiens
2.7.1.74
0.0257
-
thymidine
mutant R104Q/D133G, pH 7.5, 30°C
Homo sapiens
2.7.1.74
0.031
-
thymidine
mutant A100V/R104M/D133A, pH 7.5, 37°C
Homo sapiens
2.7.1.74
0.036
-
thymidine
mutant R104Q/D133N, pH 7.5, 30°C
Homo sapiens
2.7.1.74
0.071
-
deoxycytidine
mutant A100V/R104M/D133T, pH 7.5, 37°C
Homo sapiens
2.7.1.74
0.074
-
thymidine
mutant R104M/D133A, pH 7.5, 37°C
Homo sapiens
2.7.1.74
0.079
-
2'-deoxyguanosine
mutant D47E/R104Q/D133G/N163I/F242L, pH 7.5, 30°C
Homo sapiens
2.7.1.74
0.081
-
2'-deoxyadenosine
wild-type, pH 7.5, 30°C; wild-type, pH 7.5, 37°C
Homo sapiens
2.7.1.74
0.081
-
2'-deoxyguanosine
mutant R104Q/D133G, pH 7.5, 30°C
Homo sapiens
2.7.1.74
0.091
-
2'-deoxyadenosine
mutant D47E/R104Q/D133G/N163I/F242L, pH 7.5, 30°C
Homo sapiens
2.7.1.74
0.154
-
2'-deoxyguanosine
wild-type, pH 7.5, 37°C
Homo sapiens
2.7.1.74
0.155
-
2'-deoxyguanosine
wild-type, pH 7.5, 30°C
Homo sapiens
2.7.1.74
0.231
-
2'-deoxyadenosine
mutant R104Q/D133G, pH 7.5, 30°C
Homo sapiens
2.7.1.74
0.24
-
2'-deoxyguanosine
mutant R104Q/D133N, pH 7.5, 30°C
Homo sapiens
2.7.1.74
0.324
-
2'-deoxyguanosine
mutant R104M/D133N, pH 7.5, 37°C
Homo sapiens
2.7.1.74
0.398
-
2'-deoxyadenosine
mutant R104M/D133S, pH 7.5, 37°C
Homo sapiens
2.7.1.74
0.412
-
2'-deoxyadenosine
mutant R104M/D133A, pH 7.5, 37°C
Homo sapiens
2.7.1.74
0.452
-
2'-deoxyadenosine
mutant R104M/D133N, pH 7.5, 37°C
Homo sapiens
2.7.1.74
0.532
-
2'-deoxyadenosine
mutant R104Q/D133N, pH 7.5, 30°C
Homo sapiens
2.7.1.74
0.598
-
2'-deoxyadenosine
mutant A100V/R104M/D133A, pH 7.5, 37°C
Homo sapiens
2.7.1.74
0.691
-
2'-deoxyadenosine
mutant R104M/D133T, pH 7.5, 37°C
Homo sapiens
2.7.1.74
0.739
-
2'-deoxyguanosine
mutant A100V/R104M/D133S, pH 7.5, 37°C
Homo sapiens
2.7.1.74
0.843
-
2'-deoxyadenosine
mutant A100V/R104M/D133S, pH 7.5, 37°C
Homo sapiens
2.7.1.74
1.164
-
2'-deoxyguanosine
mutant A100V/R104M/D133T, pH 7.5, 37°C
Homo sapiens
2.7.1.74
1.172
-
2'-deoxyguanosine
mutant R104M/D133S, pH 7.5, 37°C
Homo sapiens
2.7.1.74
1.203
-
2'-deoxyguanosine
mutant R104M/D133T, pH 7.5, 37°C
Homo sapiens
2.7.1.74
1.28
-
2'-deoxyguanosine
mutant R104M/D133A, pH 7.5, 37°C
Homo sapiens
2.7.1.74
1.364
-
2'-deoxyguanosine
mutant A100V/R104M/D133A, pH 7.5, 37°C
Homo sapiens
2.7.1.74
1.437
-
2'-deoxyadenosine
mutant A100V/R104M/D133T, pH 7.5, 37°C
Homo sapiens
2.7.1.74
3.485
-
thymidine
wild-type, pH 7.5, 30°C; wild-type, pH 7.5, 37°C
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.74
Homo sapiens
P27707
-
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.74
6.5
-
substrate dideoxythymidine, wild-type, pH 7.5, 37°C
Homo sapiens
2.7.1.74
26
-
substrate 2'-deoxy-3'-thiacytidine, mutant A100V/R104M/D133T, pH 7.5, 37°C
Homo sapiens
2.7.1.74
28
-
substrate dideoxythymidine, mutant R104M/D133S, pH 7.5, 37°C
Homo sapiens
2.7.1.74
64
-
substrate dideoxythymidine, mutant A100V/R104M/D133A, pH 7.5, 37°C
Homo sapiens
2.7.1.74
88
-
substrate 2'-deoxy-3'-thiacytidine, wild-type, pH 7.5, 37°C
Homo sapiens
2.7.1.74
108
-
substrate dideoxythymidine, mutant R104M/D133T, pH 7.5, 37°C
Homo sapiens
2.7.1.74
114
-
substrate 2'-deoxy-3'-thiacytidine, mutant R104M/D133T, pH 7.5, 37°C
Homo sapiens
2.7.1.74
135
-
substrate dideoxythymidine, mutant A100V/R104M/D133T, pH 7.5, 37°C
Homo sapiens
2.7.1.74
149
-
substrate 2'-deoxy-3'-thiacytidine, mutant A100V/R104M/D133A, pH 7.5, 37°C
Homo sapiens
2.7.1.74
186
-
substrate dideoxythymidine, mutant A100V/R104M/D133S, pH 7.5, 37°C
Homo sapiens
2.7.1.74
206
-
substrate dideoxythymidine, mutant D47E/R104Q/D133G/N163I/F242L, pH 7.5, 37°C
Homo sapiens
2.7.1.74
270
-
substrate 2'-deoxy-3'-thiacytidine, mutant A100V/R104M/D133S, pH 7.5, 37°C
Homo sapiens
2.7.1.74
545
-
substrate 2'-deoxy-3'-thiacytidine, mutant R104M/D133S, pH 7.5, 37°C
Homo sapiens
2.7.1.74
599
-
substrate 1-beta-D-arabinosylcytosine, mutant A100V/R104M/D133T, pH 7.5, 37°C
Homo sapiens
2.7.1.74
815
-
substrate 2'-deoxy-3'-thiacytidine, mutant D47E/R104Q/D133G/N163I/F242L, pH 7.5, 37°C
Homo sapiens
2.7.1.74
984
-
substrate 1-beta-D-arabinosylcytosine, wild-type, pH 7.5, 37°C
Homo sapiens
2.7.1.74
1387
-
substrate 1-beta-D-arabinosylcytosine, mutant R104M/D133T, pH 7.5, 37°C
Homo sapiens
2.7.1.74
1648
-
substrate gemcitabine, wild-type, pH 7.5, 37°C
Homo sapiens
2.7.1.74
2262
-
substrate gemcitabine, mutant A100V/R104M/D133T, pH 7.5, 37°C
Homo sapiens
2.7.1.74
3177
-
substrate 1-beta-D-arabinosylcytosine, mutant A100V/R104M/D133S, pH 7.5, 37°C
Homo sapiens
2.7.1.74
3308
-
substrate 1-beta-D-arabinosylcytosine, mutant A100V/R104M/D133A, pH 7.5, 37°C
Homo sapiens
2.7.1.74
3339
-
substrate 1-beta-D-arabinosylcytosine, mutant R104M/D133SA, pH 7.5, 37°C
Homo sapiens
2.7.1.74
4267
-
substrate gemcitabine, mutant R104M/D133T, pH 7.5, 37°C
Homo sapiens
2.7.1.74
4400
-
substrate 1-beta-D-arabinosylcytosine, mutant D47E/R104Q/D133G/N163I/F242L, pH 7.5, 37°C
Homo sapiens
2.7.1.74
4530
-
substrate gemcitabine, mutant A100V/R104M/D133A, pH 7.5, 37°C
Homo sapiens
2.7.1.74
5624
-
substrate gemcitabine, mutant R104M/D133SA, pH 7.5, 37°C
Homo sapiens
2.7.1.74
6009
-
substrate gemcitabine, mutant A100V/R104M/D133S, pH 7.5, 37°C
Homo sapiens
2.7.1.74
6460
-
substrate gemcitabine, mutant D47E/R104Q/D133G/N163I/F242L, pH 7.5, 37°C
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.74
ATP + 1-beta-D-arabinosylcytosine
-
690954
Homo sapiens
ADP + 1-beta-D-arabinosylcytosine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + 2'-deoxy-3'-thiacytidine
-
690954
Homo sapiens
ADP + 2'-deoxy-3'-thiacytidine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + 2'-deoxyadenosine
-
690954
Homo sapiens
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + 2'-deoxycytidine
-
690954
Homo sapiens
ADP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + 2'-deoxyguanosine
-
690954
Homo sapiens
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + dideoxythymidine
-
690954
Homo sapiens
ADP + dideoxythymidine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + gemcitabine
-
690954
Homo sapiens
ADP + gemcitabine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + thymidine
-
690954
Homo sapiens
ADP + TMP
-
-
-
?
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.7.1.74
0.064
-
deoxycytidine
wild-type, pH 7.5, 30°C; wild-type, pH 7.5, 37°C
Homo sapiens
2.7.1.74
0.21
-
deoxycytidine
mutant D47E/R104Q/D133G/N163I/F242L, pH 7.5, 30°C
Homo sapiens
2.7.1.74
0.31
-
2'-deoxyguanosine
mutant A100V/R104M/D133A, pH 7.5, 37°C
Homo sapiens
2.7.1.74
0.37
-
deoxycytidine
mutant R104Q/D133G, pH 7.5, 30°C
Homo sapiens
2.7.1.74
0.38
-
thymidine
wild-type, pH 7.5, 30°C; wild-type, pH 7.5, 37°C
Homo sapiens
2.7.1.74
0.58
-
deoxycytidine
mutant R104M/D133A, pH 7.5, 37°C
Homo sapiens
2.7.1.74
0.68
-
thymidine
mutant D47E/R104Q/D133G/N163I/F242L, pH 7.5, 30°C
Homo sapiens
2.7.1.74
1.01
-
thymidine
mutant R104Q/D133G, pH 7.5, 30°C
Homo sapiens
2.7.1.74
1.08
-
2'-deoxyadenosine
mutant D47E/R104Q/D133G/N163I/F242L, pH 7.5, 30°C
Homo sapiens
2.7.1.74
1.18
-
2'-deoxyguanosine
mutant D47E/R104Q/D133G/N163I/F242L, pH 7.5, 30°C
Homo sapiens
2.7.1.74
1.2
-
deoxycytidine
mutant A100V/R104M/D133A, pH 7.5, 37°C
Homo sapiens
2.7.1.74
1.23
-
2'-deoxyguanosine
mutant R104M/D133A, pH 7.5, 37°C
Homo sapiens
2.7.1.74
1.3
-
deoxycytidine
mutant R104Q/D133N, pH 7.5, 30°C
Homo sapiens
2.7.1.74
1.49
-
thymidine
mutant R104M/D133T, pH 7.5, 37°C
Homo sapiens
2.7.1.74
1.5
-
2'-deoxyguanosine
mutant R104Q/D133G, pH 7.5, 30°C
Homo sapiens
2.7.1.74
1.51
-
thymidine
mutant R104Q/D133N, pH 7.5, 30°C
Homo sapiens
2.7.1.74
1.57
-
thymidine
mutant A100V/R104M/D133T, pH 7.5, 37°C
Homo sapiens
2.7.1.74
1.7
-
deoxycytidine
mutant R104M/D133S, pH 7.5, 37°C
Homo sapiens
2.7.1.74
1.72
-
2'-deoxyguanosine
mutant A100V/R104M/D133S, pH 7.5, 37°C
Homo sapiens
2.7.1.74
1.75
-
2'-deoxyadenosine
mutant R104Q/D133G, pH 7.5, 30°C
Homo sapiens
2.7.1.74
1.88
-
thymidine
mutant R104M/D133A, pH 7.5, 37°C
Homo sapiens
2.7.1.74
1.91
-
thymidine
mutant A100V/R104M/D133S, pH 7.5, 37°C
Homo sapiens
2.7.1.74
1.92
-
2'-deoxyguanosine
mutant R104Q/D133N, pH 7.5, 30°C
Homo sapiens
2.7.1.74
2
-
2'-deoxyadenosine
mutant R104Q/D133N, pH 7.5, 30°C
Homo sapiens
2.7.1.74
2.1
-
deoxycytidine
mutant A100V/R104M/D133S, pH 7.5, 37°C
Homo sapiens
2.7.1.74
2.23
-
deoxycytidine
mutant R104M/D133N, pH 7.5, 37°C
Homo sapiens
2.7.1.74
2.27
-
thymidine
mutant R104M/D133N, pH 7.5, 37°C
Homo sapiens
2.7.1.74
2.29
-
2'-deoxyguanosine
mutant A100V/R104M/D133T, pH 7.5, 37°C
Homo sapiens
2.7.1.74
2.33
-
thymidine
mutant R104M/D133S, pH 7.5, 37°C
Homo sapiens
2.7.1.74
2.5
-
2'-deoxyguanosine
mutant R104M/D133T, pH 7.5, 37°C
Homo sapiens
2.7.1.74
2.67
-
2'-deoxyadenosine
mutant A100V/R104M/D133T, pH 7.5, 37°C
Homo sapiens
2.7.1.74
2.71
-
2'-deoxyadenosine
mutant R104M/D133A, pH 7.5, 37°C
Homo sapiens
2.7.1.74
2.86
-
deoxycytidine
mutant R104M/D133T, pH 7.5, 37°C
Homo sapiens
2.7.1.74
3.1
-
2'-deoxyadenosine
wild-type, pH 7.5, 30°C; wild-type, pH 7.5, 37°C
Homo sapiens
2.7.1.74
3.26
-
2'-deoxyguanosine
wild-type, pH 7.5, 30°C; wild-type, pH 7.5, 37°C
Homo sapiens
2.7.1.74
3.29
-
thymidine
mutant A100V/R104M/D133A, pH 7.5, 37°C
Homo sapiens
2.7.1.74
3.37
-
2'-deoxyadenosine
mutant A100V/R104M/D133S, pH 7.5, 37°C
Homo sapiens
2.7.1.74
3.4
-
2'-deoxyadenosine
mutant A100V/R104M/D133A, pH 7.5, 37°C
Homo sapiens
2.7.1.74
3.47
-
2'-deoxyadenosine
mutant R104M/D133N, pH 7.5, 37°C
Homo sapiens
2.7.1.74
3.54
-
deoxycytidine
mutant A100V/R104M/D133T, pH 7.5, 37°C
Homo sapiens
2.7.1.74
3.59
-
2'-deoxyguanosine
mutant R104M/D133N, pH 7.5, 37°C
Homo sapiens
2.7.1.74
4.14
-
2'-deoxyadenosine
mutant R104M/D133T, pH 7.5, 37°C
Homo sapiens
2.7.1.74
4.38
-
2'-deoxyguanosine
mutant R104M/D133S, pH 7.5, 37°C
Homo sapiens
2.7.1.74
4.85
-
2'-deoxyadenosine
mutant R104M/D133S, pH 7.5, 37°C
Homo sapiens
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
2.7.1.74
A100V/R104M/D133A
12fold increase in ratio kcat/Km for deoxycytidine
Homo sapiens
2.7.1.74
A100V/R104M/D133S
6fold increase in ratio kcat/Km for deoxycytidine
Homo sapiens
2.7.1.74
A100V/R104M/D133T
ratio kcat/Km for deoxycytidine similar to wild-type
Homo sapiens
2.7.1.74
D47E/R104Q/D133G/N163I/F242L
1.5fold increase in ratio kcat/Km for deoxycytidine
Homo sapiens
2.7.1.74
additional information
R104 and D133 are key residues for substrate specificity
Homo sapiens
2.7.1.74
R104M/D133A
4fold increase in ratio kcat/Km for deoxycytidine
Homo sapiens
2.7.1.74
R104M/D133N
3fold increase in ratio kcat/Km for deoxycytidine
Homo sapiens
2.7.1.74
R104M/D133S
6fold increase in ratio kcat/Km for deoxycytidine
Homo sapiens
2.7.1.74
R104M/D133T
mutant with reversed substrate specificity, with elevated specific constant for thymidine phosphorylation and decreased activity for deeoxycytidine, deoxyadenosine, and deoxyguanosine
Homo sapiens
2.7.1.74
R104Q/D133G
mutant is a generalist kinase with broader specificity and elevated turnover compared with wild-type
Homo sapiens
2.7.1.74
R104Q/D133N
1.5fold increase in ratio kcat/Km for deoxycytidine
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.74
0.001
-
deoxycytidine
wild-type, pH 7.5, 30°C; wild-type, pH 7.5, 37°C
Homo sapiens
2.7.1.74
0.00104
-
deoxycytidine
mutant R104Q/D133G, pH 7.5, 30°C
Homo sapiens
2.7.1.74
0.00141
-
deoxycytidine
mutant D47E/R104Q/D133G/N163I/F242L, pH 7.5, 30°C
Homo sapiens
2.7.1.74
0.00154
-
deoxycytidine
mutant A100V/R104M/D133A, pH 7.5, 37°C
Homo sapiens
2.7.1.74
0.002
-
deoxycytidine
mutant R104M/D133A, pH 7.5, 37°C
Homo sapiens
2.7.1.74
0.00388
-
thymidine
mutant A100V/R104M/D133T, pH 7.5, 37°C
Homo sapiens
2.7.1.74
0.00455
-
deoxycytidine
mutant R104M/D133S, pH 7.5, 37°C
Homo sapiens
2.7.1.74
0.00525
-
deoxycytidine
mutant A100V/R104M/D133S, pH 7.5, 37°C
Homo sapiens
2.7.1.74
0.00558
-
thymidine
mutant A100V/R104M/D133S, pH 7.5, 37°C
Homo sapiens
2.7.1.74
0.0058
-
thymidine
mutant R104M/D133T, pH 7.5, 37°C
Homo sapiens
2.7.1.74
0.0086
-
deoxycytidine
mutant R104Q/D133N, pH 7.5, 30°C
Homo sapiens
2.7.1.74
0.0118
-
deoxycytidine
mutant R104M/D133N, pH 7.5, 37°C
Homo sapiens
2.7.1.74
0.0189
-
thymidine
mutant R104M/D133S, pH 7.5, 37°C
Homo sapiens
2.7.1.74
0.0209
-
deoxycytidine
mutant R104M/D133T, pH 7.5, 37°C
Homo sapiens
2.7.1.74
0.025
-
thymidine
mutant D47E/R104Q/D133G/N163I/F242L, pH 7.5, 30°C
Homo sapiens
2.7.1.74
0.0256
-
thymidine
mutant R104M/D133N, pH 7.5, 37°C
Homo sapiens
2.7.1.74
0.0257
-
thymidine
mutant R104Q/D133G, pH 7.5, 30°C
Homo sapiens
2.7.1.74
0.031
-
thymidine
mutant A100V/R104M/D133A, pH 7.5, 37°C
Homo sapiens
2.7.1.74
0.036
-
thymidine
mutant R104Q/D133N, pH 7.5, 30°C
Homo sapiens
2.7.1.74
0.071
-
deoxycytidine
mutant A100V/R104M/D133T, pH 7.5, 37°C
Homo sapiens
2.7.1.74
0.074
-
thymidine
mutant R104M/D133A, pH 7.5, 37°C
Homo sapiens
2.7.1.74
0.079
-
2'-deoxyguanosine
mutant D47E/R104Q/D133G/N163I/F242L, pH 7.5, 30°C
Homo sapiens
2.7.1.74
0.081
-
2'-deoxyadenosine
wild-type, pH 7.5, 30°C; wild-type, pH 7.5, 37°C
Homo sapiens
2.7.1.74
0.081
-
2'-deoxyguanosine
mutant R104Q/D133G, pH 7.5, 30°C
Homo sapiens
2.7.1.74
0.091
-
2'-deoxyadenosine
mutant D47E/R104Q/D133G/N163I/F242L, pH 7.5, 30°C
Homo sapiens
2.7.1.74
0.154
-
2'-deoxyguanosine
wild-type, pH 7.5, 37°C
Homo sapiens
2.7.1.74
0.155
-
2'-deoxyguanosine
wild-type, pH 7.5, 30°C
Homo sapiens
2.7.1.74
0.231
-
2'-deoxyadenosine
mutant R104Q/D133G, pH 7.5, 30°C
Homo sapiens
2.7.1.74
0.24
-
2'-deoxyguanosine
mutant R104Q/D133N, pH 7.5, 30°C
Homo sapiens
2.7.1.74
0.324
-
2'-deoxyguanosine
mutant R104M/D133N, pH 7.5, 37°C
Homo sapiens
2.7.1.74
0.398
-
2'-deoxyadenosine
mutant R104M/D133S, pH 7.5, 37°C
Homo sapiens
2.7.1.74
0.412
-
2'-deoxyadenosine
mutant R104M/D133A, pH 7.5, 37°C
Homo sapiens
2.7.1.74
0.452
-
2'-deoxyadenosine
mutant R104M/D133N, pH 7.5, 37°C
Homo sapiens
2.7.1.74
0.532
-
2'-deoxyadenosine
mutant R104Q/D133N, pH 7.5, 30°C
Homo sapiens
2.7.1.74
0.598
-
2'-deoxyadenosine
mutant A100V/R104M/D133A, pH 7.5, 37°C
Homo sapiens
2.7.1.74
0.691
-
2'-deoxyadenosine
mutant R104M/D133T, pH 7.5, 37°C
Homo sapiens
2.7.1.74
0.739
-
2'-deoxyguanosine
mutant A100V/R104M/D133S, pH 7.5, 37°C
Homo sapiens
2.7.1.74
0.843
-
2'-deoxyadenosine
mutant A100V/R104M/D133S, pH 7.5, 37°C
Homo sapiens
2.7.1.74
1.164
-
2'-deoxyguanosine
mutant A100V/R104M/D133T, pH 7.5, 37°C
Homo sapiens
2.7.1.74
1.172
-
2'-deoxyguanosine
mutant R104M/D133S, pH 7.5, 37°C
Homo sapiens
2.7.1.74
1.203
-
2'-deoxyguanosine
mutant R104M/D133T, pH 7.5, 37°C
Homo sapiens
2.7.1.74
1.28
-
2'-deoxyguanosine
mutant R104M/D133A, pH 7.5, 37°C
Homo sapiens
2.7.1.74
1.364
-
2'-deoxyguanosine
mutant A100V/R104M/D133A, pH 7.5, 37°C
Homo sapiens
2.7.1.74
1.437
-
2'-deoxyadenosine
mutant A100V/R104M/D133T, pH 7.5, 37°C
Homo sapiens
2.7.1.74
3.485
-
thymidine
wild-type, pH 7.5, 30°C; wild-type, pH 7.5, 37°C
Homo sapiens
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.74
6.5
-
substrate dideoxythymidine, wild-type, pH 7.5, 37°C
Homo sapiens
2.7.1.74
26
-
substrate 2'-deoxy-3'-thiacytidine, mutant A100V/R104M/D133T, pH 7.5, 37°C
Homo sapiens
2.7.1.74
28
-
substrate dideoxythymidine, mutant R104M/D133S, pH 7.5, 37°C
Homo sapiens
2.7.1.74
64
-
substrate dideoxythymidine, mutant A100V/R104M/D133A, pH 7.5, 37°C
Homo sapiens
2.7.1.74
88
-
substrate 2'-deoxy-3'-thiacytidine, wild-type, pH 7.5, 37°C
Homo sapiens
2.7.1.74
108
-
substrate dideoxythymidine, mutant R104M/D133T, pH 7.5, 37°C
Homo sapiens
2.7.1.74
114
-
substrate 2'-deoxy-3'-thiacytidine, mutant R104M/D133T, pH 7.5, 37°C
Homo sapiens
2.7.1.74
135
-
substrate dideoxythymidine, mutant A100V/R104M/D133T, pH 7.5, 37°C
Homo sapiens
2.7.1.74
149
-
substrate 2'-deoxy-3'-thiacytidine, mutant A100V/R104M/D133A, pH 7.5, 37°C
Homo sapiens
2.7.1.74
186
-
substrate dideoxythymidine, mutant A100V/R104M/D133S, pH 7.5, 37°C
Homo sapiens
2.7.1.74
206
-
substrate dideoxythymidine, mutant D47E/R104Q/D133G/N163I/F242L, pH 7.5, 37°C
Homo sapiens
2.7.1.74
270
-
substrate 2'-deoxy-3'-thiacytidine, mutant A100V/R104M/D133S, pH 7.5, 37°C
Homo sapiens
2.7.1.74
545
-
substrate 2'-deoxy-3'-thiacytidine, mutant R104M/D133S, pH 7.5, 37°C
Homo sapiens
2.7.1.74
599
-
substrate 1-beta-D-arabinosylcytosine, mutant A100V/R104M/D133T, pH 7.5, 37°C
Homo sapiens
2.7.1.74
815
-
substrate 2'-deoxy-3'-thiacytidine, mutant D47E/R104Q/D133G/N163I/F242L, pH 7.5, 37°C
Homo sapiens
2.7.1.74
984
-
substrate 1-beta-D-arabinosylcytosine, wild-type, pH 7.5, 37°C
Homo sapiens
2.7.1.74
1387
-
substrate 1-beta-D-arabinosylcytosine, mutant R104M/D133T, pH 7.5, 37°C
Homo sapiens
2.7.1.74
1648
-
substrate gemcitabine, wild-type, pH 7.5, 37°C
Homo sapiens
2.7.1.74
2262
-
substrate gemcitabine, mutant A100V/R104M/D133T, pH 7.5, 37°C
Homo sapiens
2.7.1.74
3177
-
substrate 1-beta-D-arabinosylcytosine, mutant A100V/R104M/D133S, pH 7.5, 37°C
Homo sapiens
2.7.1.74
3308
-
substrate 1-beta-D-arabinosylcytosine, mutant A100V/R104M/D133A, pH 7.5, 37°C
Homo sapiens
2.7.1.74
3339
-
substrate 1-beta-D-arabinosylcytosine, mutant R104M/D133SA, pH 7.5, 37°C
Homo sapiens
2.7.1.74
4267
-
substrate gemcitabine, mutant R104M/D133T, pH 7.5, 37°C
Homo sapiens
2.7.1.74
4400
-
substrate 1-beta-D-arabinosylcytosine, mutant D47E/R104Q/D133G/N163I/F242L, pH 7.5, 37°C
Homo sapiens
2.7.1.74
4530
-
substrate gemcitabine, mutant A100V/R104M/D133A, pH 7.5, 37°C
Homo sapiens
2.7.1.74
5624
-
substrate gemcitabine, mutant R104M/D133SA, pH 7.5, 37°C
Homo sapiens
2.7.1.74
6009
-
substrate gemcitabine, mutant A100V/R104M/D133S, pH 7.5, 37°C
Homo sapiens
2.7.1.74
6460
-
substrate gemcitabine, mutant D47E/R104Q/D133G/N163I/F242L, pH 7.5, 37°C
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.74
ATP + 1-beta-D-arabinosylcytosine
-
690954
Homo sapiens
ADP + 1-beta-D-arabinosylcytosine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + 2'-deoxy-3'-thiacytidine
-
690954
Homo sapiens
ADP + 2'-deoxy-3'-thiacytidine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + 2'-deoxyadenosine
-
690954
Homo sapiens
ADP + 2'-deoxyadenosine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + 2'-deoxycytidine
-
690954
Homo sapiens
ADP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + 2'-deoxyguanosine
-
690954
Homo sapiens
ADP + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + dideoxythymidine
-
690954
Homo sapiens
ADP + dideoxythymidine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + gemcitabine
-
690954
Homo sapiens
ADP + gemcitabine 5'-phosphate
-
-
-
?
2.7.1.74
ATP + thymidine
-
690954
Homo sapiens
ADP + TMP
-
-
-
?
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.7.1.74
0.064
-
deoxycytidine
wild-type, pH 7.5, 30°C; wild-type, pH 7.5, 37°C
Homo sapiens
2.7.1.74
0.21
-
deoxycytidine
mutant D47E/R104Q/D133G/N163I/F242L, pH 7.5, 30°C
Homo sapiens
2.7.1.74
0.31
-
2'-deoxyguanosine
mutant A100V/R104M/D133A, pH 7.5, 37°C
Homo sapiens
2.7.1.74
0.37
-
deoxycytidine
mutant R104Q/D133G, pH 7.5, 30°C
Homo sapiens
2.7.1.74
0.38
-
thymidine
wild-type, pH 7.5, 30°C; wild-type, pH 7.5, 37°C
Homo sapiens
2.7.1.74
0.58
-
deoxycytidine
mutant R104M/D133A, pH 7.5, 37°C
Homo sapiens
2.7.1.74
0.68
-
thymidine
mutant D47E/R104Q/D133G/N163I/F242L, pH 7.5, 30°C
Homo sapiens
2.7.1.74
1.01
-
thymidine
mutant R104Q/D133G, pH 7.5, 30°C
Homo sapiens
2.7.1.74
1.08
-
2'-deoxyadenosine
mutant D47E/R104Q/D133G/N163I/F242L, pH 7.5, 30°C
Homo sapiens
2.7.1.74
1.18
-
2'-deoxyguanosine
mutant D47E/R104Q/D133G/N163I/F242L, pH 7.5, 30°C
Homo sapiens
2.7.1.74
1.2
-
deoxycytidine
mutant A100V/R104M/D133A, pH 7.5, 37°C
Homo sapiens
2.7.1.74
1.23
-
2'-deoxyguanosine
mutant R104M/D133A, pH 7.5, 37°C
Homo sapiens
2.7.1.74
1.3
-
deoxycytidine
mutant R104Q/D133N, pH 7.5, 30°C
Homo sapiens
2.7.1.74
1.49
-
thymidine
mutant R104M/D133T, pH 7.5, 37°C
Homo sapiens
2.7.1.74
1.5
-
2'-deoxyguanosine
mutant R104Q/D133G, pH 7.5, 30°C
Homo sapiens
2.7.1.74
1.51
-
thymidine
mutant R104Q/D133N, pH 7.5, 30°C
Homo sapiens
2.7.1.74
1.57
-
thymidine
mutant A100V/R104M/D133T, pH 7.5, 37°C
Homo sapiens
2.7.1.74
1.7
-
deoxycytidine
mutant R104M/D133S, pH 7.5, 37°C
Homo sapiens
2.7.1.74
1.72
-
2'-deoxyguanosine
mutant A100V/R104M/D133S, pH 7.5, 37°C
Homo sapiens
2.7.1.74
1.75
-
2'-deoxyadenosine
mutant R104Q/D133G, pH 7.5, 30°C
Homo sapiens
2.7.1.74
1.88
-
thymidine
mutant R104M/D133A, pH 7.5, 37°C
Homo sapiens
2.7.1.74
1.91
-
thymidine
mutant A100V/R104M/D133S, pH 7.5, 37°C
Homo sapiens
2.7.1.74
1.92
-
2'-deoxyguanosine
mutant R104Q/D133N, pH 7.5, 30°C
Homo sapiens
2.7.1.74
2
-
2'-deoxyadenosine
mutant R104Q/D133N, pH 7.5, 30°C
Homo sapiens
2.7.1.74
2.1
-
deoxycytidine
mutant A100V/R104M/D133S, pH 7.5, 37°C
Homo sapiens
2.7.1.74
2.23
-
deoxycytidine
mutant R104M/D133N, pH 7.5, 37°C
Homo sapiens
2.7.1.74
2.27
-
thymidine
mutant R104M/D133N, pH 7.5, 37°C
Homo sapiens
2.7.1.74
2.29
-
2'-deoxyguanosine
mutant A100V/R104M/D133T, pH 7.5, 37°C
Homo sapiens
2.7.1.74
2.33
-
thymidine
mutant R104M/D133S, pH 7.5, 37°C
Homo sapiens
2.7.1.74
2.5
-
2'-deoxyguanosine
mutant R104M/D133T, pH 7.5, 37°C
Homo sapiens
2.7.1.74
2.67
-
2'-deoxyadenosine
mutant A100V/R104M/D133T, pH 7.5, 37°C
Homo sapiens
2.7.1.74
2.71
-
2'-deoxyadenosine
mutant R104M/D133A, pH 7.5, 37°C
Homo sapiens
2.7.1.74
2.86
-
deoxycytidine
mutant R104M/D133T, pH 7.5, 37°C
Homo sapiens
2.7.1.74
3.1
-
2'-deoxyadenosine
wild-type, pH 7.5, 30°C; wild-type, pH 7.5, 37°C
Homo sapiens
2.7.1.74
3.26
-
2'-deoxyguanosine
wild-type, pH 7.5, 30°C; wild-type, pH 7.5, 37°C
Homo sapiens
2.7.1.74
3.29
-
thymidine
mutant A100V/R104M/D133A, pH 7.5, 37°C
Homo sapiens
2.7.1.74
3.37
-
2'-deoxyadenosine
mutant A100V/R104M/D133S, pH 7.5, 37°C
Homo sapiens
2.7.1.74
3.4
-
2'-deoxyadenosine
mutant A100V/R104M/D133A, pH 7.5, 37°C
Homo sapiens
2.7.1.74
3.47
-
2'-deoxyadenosine
mutant R104M/D133N, pH 7.5, 37°C
Homo sapiens
2.7.1.74
3.54
-
deoxycytidine
mutant A100V/R104M/D133T, pH 7.5, 37°C
Homo sapiens
2.7.1.74
3.59
-
2'-deoxyguanosine
mutant R104M/D133N, pH 7.5, 37°C
Homo sapiens
2.7.1.74
4.14
-
2'-deoxyadenosine
mutant R104M/D133T, pH 7.5, 37°C
Homo sapiens
2.7.1.74
4.38
-
2'-deoxyguanosine
mutant R104M/D133S, pH 7.5, 37°C
Homo sapiens
2.7.1.74
4.85
-
2'-deoxyadenosine
mutant R104M/D133S, pH 7.5, 37°C
Homo sapiens