EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.7.1.74 | A100V/R104M/D133A | 12fold increase in ratio kcat/Km for deoxycytidine | Homo sapiens |
2.7.1.74 | A100V/R104M/D133S | 6fold increase in ratio kcat/Km for deoxycytidine | Homo sapiens |
2.7.1.74 | A100V/R104M/D133T | ratio kcat/Km for deoxycytidine similar to wild-type | Homo sapiens |
2.7.1.74 | D47E/R104Q/D133G/N163I/F242L | 1.5fold increase in ratio kcat/Km for deoxycytidine | Homo sapiens |
2.7.1.74 | additional information | R104 and D133 are key residues for substrate specificity | Homo sapiens |
2.7.1.74 | R104M/D133A | 4fold increase in ratio kcat/Km for deoxycytidine | Homo sapiens |
2.7.1.74 | R104M/D133N | 3fold increase in ratio kcat/Km for deoxycytidine | Homo sapiens |
2.7.1.74 | R104M/D133S | 6fold increase in ratio kcat/Km for deoxycytidine | Homo sapiens |
2.7.1.74 | R104M/D133T | mutant with reversed substrate specificity, with elevated specific constant for thymidine phosphorylation and decreased activity for deeoxycytidine, deoxyadenosine, and deoxyguanosine | Homo sapiens |
2.7.1.74 | R104Q/D133G | mutant is a generalist kinase with broader specificity and elevated turnover compared with wild-type | Homo sapiens |
2.7.1.74 | R104Q/D133N | 1.5fold increase in ratio kcat/Km for deoxycytidine | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.74 | 0.001 | - |
deoxycytidine | wild-type, pH 7.5, 37°C | Homo sapiens | |
2.7.1.74 | 0.001 | - |
deoxycytidine | wild-type, pH 7.5, 30°C | Homo sapiens | |
2.7.1.74 | 0.00104 | - |
deoxycytidine | mutant R104Q/D133G, pH 7.5, 30°C | Homo sapiens | |
2.7.1.74 | 0.00141 | - |
deoxycytidine | mutant D47E/R104Q/D133G/N163I/F242L, pH 7.5, 30°C | Homo sapiens | |
2.7.1.74 | 0.00154 | - |
deoxycytidine | mutant A100V/R104M/D133A, pH 7.5, 37°C | Homo sapiens | |
2.7.1.74 | 0.002 | - |
deoxycytidine | mutant R104M/D133A, pH 7.5, 37°C | Homo sapiens | |
2.7.1.74 | 0.00388 | - |
thymidine | mutant A100V/R104M/D133T, pH 7.5, 37°C | Homo sapiens | |
2.7.1.74 | 0.00455 | - |
deoxycytidine | mutant R104M/D133S, pH 7.5, 37°C | Homo sapiens | |
2.7.1.74 | 0.00525 | - |
deoxycytidine | mutant A100V/R104M/D133S, pH 7.5, 37°C | Homo sapiens | |
2.7.1.74 | 0.00558 | - |
thymidine | mutant A100V/R104M/D133S, pH 7.5, 37°C | Homo sapiens | |
2.7.1.74 | 0.0058 | - |
thymidine | mutant R104M/D133T, pH 7.5, 37°C | Homo sapiens | |
2.7.1.74 | 0.0086 | - |
deoxycytidine | mutant R104Q/D133N, pH 7.5, 30°C | Homo sapiens | |
2.7.1.74 | 0.0118 | - |
deoxycytidine | mutant R104M/D133N, pH 7.5, 37°C | Homo sapiens | |
2.7.1.74 | 0.0189 | - |
thymidine | mutant R104M/D133S, pH 7.5, 37°C | Homo sapiens | |
2.7.1.74 | 0.0209 | - |
deoxycytidine | mutant R104M/D133T, pH 7.5, 37°C | Homo sapiens | |
2.7.1.74 | 0.025 | - |
thymidine | mutant D47E/R104Q/D133G/N163I/F242L, pH 7.5, 30°C | Homo sapiens | |
2.7.1.74 | 0.0256 | - |
thymidine | mutant R104M/D133N, pH 7.5, 37°C | Homo sapiens | |
2.7.1.74 | 0.0257 | - |
thymidine | mutant R104Q/D133G, pH 7.5, 30°C | Homo sapiens | |
2.7.1.74 | 0.031 | - |
thymidine | mutant A100V/R104M/D133A, pH 7.5, 37°C | Homo sapiens | |
2.7.1.74 | 0.036 | - |
thymidine | mutant R104Q/D133N, pH 7.5, 30°C | Homo sapiens | |
2.7.1.74 | 0.071 | - |
deoxycytidine | mutant A100V/R104M/D133T, pH 7.5, 37°C | Homo sapiens | |
2.7.1.74 | 0.074 | - |
thymidine | mutant R104M/D133A, pH 7.5, 37°C | Homo sapiens | |
2.7.1.74 | 0.079 | - |
2'-deoxyguanosine | mutant D47E/R104Q/D133G/N163I/F242L, pH 7.5, 30°C | Homo sapiens | |
2.7.1.74 | 0.081 | - |
2'-deoxyadenosine | wild-type, pH 7.5, 37°C | Homo sapiens | |
2.7.1.74 | 0.081 | - |
2'-deoxyadenosine | wild-type, pH 7.5, 30°C | Homo sapiens | |
2.7.1.74 | 0.081 | - |
2'-deoxyguanosine | mutant R104Q/D133G, pH 7.5, 30°C | Homo sapiens | |
2.7.1.74 | 0.091 | - |
2'-deoxyadenosine | mutant D47E/R104Q/D133G/N163I/F242L, pH 7.5, 30°C | Homo sapiens | |
2.7.1.74 | 0.154 | - |
2'-deoxyguanosine | wild-type, pH 7.5, 37°C | Homo sapiens | |
2.7.1.74 | 0.155 | - |
2'-deoxyguanosine | wild-type, pH 7.5, 30°C | Homo sapiens | |
2.7.1.74 | 0.231 | - |
2'-deoxyadenosine | mutant R104Q/D133G, pH 7.5, 30°C | Homo sapiens | |
2.7.1.74 | 0.24 | - |
2'-deoxyguanosine | mutant R104Q/D133N, pH 7.5, 30°C | Homo sapiens | |
2.7.1.74 | 0.324 | - |
2'-deoxyguanosine | mutant R104M/D133N, pH 7.5, 37°C | Homo sapiens | |
2.7.1.74 | 0.398 | - |
2'-deoxyadenosine | mutant R104M/D133S, pH 7.5, 37°C | Homo sapiens | |
2.7.1.74 | 0.412 | - |
2'-deoxyadenosine | mutant R104M/D133A, pH 7.5, 37°C | Homo sapiens | |
2.7.1.74 | 0.452 | - |
2'-deoxyadenosine | mutant R104M/D133N, pH 7.5, 37°C | Homo sapiens | |
2.7.1.74 | 0.532 | - |
2'-deoxyadenosine | mutant R104Q/D133N, pH 7.5, 30°C | Homo sapiens | |
2.7.1.74 | 0.598 | - |
2'-deoxyadenosine | mutant A100V/R104M/D133A, pH 7.5, 37°C | Homo sapiens | |
2.7.1.74 | 0.691 | - |
2'-deoxyadenosine | mutant R104M/D133T, pH 7.5, 37°C | Homo sapiens | |
2.7.1.74 | 0.739 | - |
2'-deoxyguanosine | mutant A100V/R104M/D133S, pH 7.5, 37°C | Homo sapiens | |
2.7.1.74 | 0.843 | - |
2'-deoxyadenosine | mutant A100V/R104M/D133S, pH 7.5, 37°C | Homo sapiens | |
2.7.1.74 | 1.164 | - |
2'-deoxyguanosine | mutant A100V/R104M/D133T, pH 7.5, 37°C | Homo sapiens | |
2.7.1.74 | 1.172 | - |
2'-deoxyguanosine | mutant R104M/D133S, pH 7.5, 37°C | Homo sapiens | |
2.7.1.74 | 1.203 | - |
2'-deoxyguanosine | mutant R104M/D133T, pH 7.5, 37°C | Homo sapiens | |
2.7.1.74 | 1.28 | - |
2'-deoxyguanosine | mutant R104M/D133A, pH 7.5, 37°C | Homo sapiens | |
2.7.1.74 | 1.364 | - |
2'-deoxyguanosine | mutant A100V/R104M/D133A, pH 7.5, 37°C | Homo sapiens | |
2.7.1.74 | 1.437 | - |
2'-deoxyadenosine | mutant A100V/R104M/D133T, pH 7.5, 37°C | Homo sapiens | |
2.7.1.74 | 3.485 | - |
thymidine | wild-type, pH 7.5, 37°C | Homo sapiens | |
2.7.1.74 | 3.485 | - |
thymidine | wild-type, pH 7.5, 30°C | Homo sapiens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.74 | Homo sapiens | P27707 | - |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.1.74 | 6.5 | - |
substrate dideoxythymidine, wild-type, pH 7.5, 37°C | Homo sapiens |
2.7.1.74 | 26 | - |
substrate 2'-deoxy-3'-thiacytidine, mutant A100V/R104M/D133T, pH 7.5, 37°C | Homo sapiens |
2.7.1.74 | 28 | - |
substrate dideoxythymidine, mutant R104M/D133S, pH 7.5, 37°C | Homo sapiens |
2.7.1.74 | 64 | - |
substrate dideoxythymidine, mutant A100V/R104M/D133A, pH 7.5, 37°C | Homo sapiens |
2.7.1.74 | 88 | - |
substrate 2'-deoxy-3'-thiacytidine, wild-type, pH 7.5, 37°C | Homo sapiens |
2.7.1.74 | 108 | - |
substrate dideoxythymidine, mutant R104M/D133T, pH 7.5, 37°C | Homo sapiens |
2.7.1.74 | 114 | - |
substrate 2'-deoxy-3'-thiacytidine, mutant R104M/D133T, pH 7.5, 37°C | Homo sapiens |
2.7.1.74 | 135 | - |
substrate dideoxythymidine, mutant A100V/R104M/D133T, pH 7.5, 37°C | Homo sapiens |
2.7.1.74 | 149 | - |
substrate 2'-deoxy-3'-thiacytidine, mutant A100V/R104M/D133A, pH 7.5, 37°C | Homo sapiens |
2.7.1.74 | 186 | - |
substrate dideoxythymidine, mutant A100V/R104M/D133S, pH 7.5, 37°C | Homo sapiens |
2.7.1.74 | 206 | - |
substrate dideoxythymidine, mutant D47E/R104Q/D133G/N163I/F242L, pH 7.5, 37°C | Homo sapiens |
2.7.1.74 | 270 | - |
substrate 2'-deoxy-3'-thiacytidine, mutant A100V/R104M/D133S, pH 7.5, 37°C | Homo sapiens |
2.7.1.74 | 545 | - |
substrate 2'-deoxy-3'-thiacytidine, mutant R104M/D133S, pH 7.5, 37°C | Homo sapiens |
2.7.1.74 | 599 | - |
substrate 1-beta-D-arabinosylcytosine, mutant A100V/R104M/D133T, pH 7.5, 37°C | Homo sapiens |
2.7.1.74 | 815 | - |
substrate 2'-deoxy-3'-thiacytidine, mutant D47E/R104Q/D133G/N163I/F242L, pH 7.5, 37°C | Homo sapiens |
2.7.1.74 | 984 | - |
substrate 1-beta-D-arabinosylcytosine, wild-type, pH 7.5, 37°C | Homo sapiens |
2.7.1.74 | 1387 | - |
substrate 1-beta-D-arabinosylcytosine, mutant R104M/D133T, pH 7.5, 37°C | Homo sapiens |
2.7.1.74 | 1648 | - |
substrate gemcitabine, wild-type, pH 7.5, 37°C | Homo sapiens |
2.7.1.74 | 2262 | - |
substrate gemcitabine, mutant A100V/R104M/D133T, pH 7.5, 37°C | Homo sapiens |
2.7.1.74 | 3177 | - |
substrate 1-beta-D-arabinosylcytosine, mutant A100V/R104M/D133S, pH 7.5, 37°C | Homo sapiens |
2.7.1.74 | 3308 | - |
substrate 1-beta-D-arabinosylcytosine, mutant A100V/R104M/D133A, pH 7.5, 37°C | Homo sapiens |
2.7.1.74 | 3339 | - |
substrate 1-beta-D-arabinosylcytosine, mutant R104M/D133SA, pH 7.5, 37°C | Homo sapiens |
2.7.1.74 | 4267 | - |
substrate gemcitabine, mutant R104M/D133T, pH 7.5, 37°C | Homo sapiens |
2.7.1.74 | 4400 | - |
substrate 1-beta-D-arabinosylcytosine, mutant D47E/R104Q/D133G/N163I/F242L, pH 7.5, 37°C | Homo sapiens |
2.7.1.74 | 4530 | - |
substrate gemcitabine, mutant A100V/R104M/D133A, pH 7.5, 37°C | Homo sapiens |
2.7.1.74 | 5624 | - |
substrate gemcitabine, mutant R104M/D133SA, pH 7.5, 37°C | Homo sapiens |
2.7.1.74 | 6009 | - |
substrate gemcitabine, mutant A100V/R104M/D133S, pH 7.5, 37°C | Homo sapiens |
2.7.1.74 | 6460 | - |
substrate gemcitabine, mutant D47E/R104Q/D133G/N163I/F242L, pH 7.5, 37°C | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.74 | ATP + 1-beta-D-arabinosylcytosine | - |
Homo sapiens | ADP + 1-beta-D-arabinosylcytosine 5'-phosphate | - |
? | |
2.7.1.74 | ATP + 2'-deoxy-3'-thiacytidine | - |
Homo sapiens | ADP + 2'-deoxy-3'-thiacytidine 5'-phosphate | - |
? | |
2.7.1.74 | ATP + 2'-deoxyadenosine | - |
Homo sapiens | ADP + 2'-deoxyadenosine 5'-phosphate | - |
? | |
2.7.1.74 | ATP + 2'-deoxycytidine | - |
Homo sapiens | ADP + 2'-deoxycytidine 5'-phosphate | - |
? | |
2.7.1.74 | ATP + 2'-deoxyguanosine | - |
Homo sapiens | ADP + 2'-deoxyguanosine 5'-phosphate | - |
? | |
2.7.1.74 | ATP + dideoxythymidine | - |
Homo sapiens | ADP + dideoxythymidine 5'-phosphate | - |
? | |
2.7.1.74 | ATP + gemcitabine | - |
Homo sapiens | ADP + gemcitabine 5'-phosphate | - |
? | |
2.7.1.74 | ATP + thymidine | - |
Homo sapiens | ADP + TMP | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.74 | 0.064 | - |
deoxycytidine | wild-type, pH 7.5, 37°C | Homo sapiens | |
2.7.1.74 | 0.064 | - |
deoxycytidine | wild-type, pH 7.5, 30°C | Homo sapiens | |
2.7.1.74 | 0.21 | - |
deoxycytidine | mutant D47E/R104Q/D133G/N163I/F242L, pH 7.5, 30°C | Homo sapiens | |
2.7.1.74 | 0.31 | - |
2'-deoxyguanosine | mutant A100V/R104M/D133A, pH 7.5, 37°C | Homo sapiens | |
2.7.1.74 | 0.37 | - |
deoxycytidine | mutant R104Q/D133G, pH 7.5, 30°C | Homo sapiens | |
2.7.1.74 | 0.38 | - |
thymidine | wild-type, pH 7.5, 37°C | Homo sapiens | |
2.7.1.74 | 0.38 | - |
thymidine | wild-type, pH 7.5, 30°C | Homo sapiens | |
2.7.1.74 | 0.58 | - |
deoxycytidine | mutant R104M/D133A, pH 7.5, 37°C | Homo sapiens | |
2.7.1.74 | 0.68 | - |
thymidine | mutant D47E/R104Q/D133G/N163I/F242L, pH 7.5, 30°C | Homo sapiens | |
2.7.1.74 | 1.01 | - |
thymidine | mutant R104Q/D133G, pH 7.5, 30°C | Homo sapiens | |
2.7.1.74 | 1.08 | - |
2'-deoxyadenosine | mutant D47E/R104Q/D133G/N163I/F242L, pH 7.5, 30°C | Homo sapiens | |
2.7.1.74 | 1.18 | - |
2'-deoxyguanosine | mutant D47E/R104Q/D133G/N163I/F242L, pH 7.5, 30°C | Homo sapiens | |
2.7.1.74 | 1.2 | - |
deoxycytidine | mutant A100V/R104M/D133A, pH 7.5, 37°C | Homo sapiens | |
2.7.1.74 | 1.23 | - |
2'-deoxyguanosine | mutant R104M/D133A, pH 7.5, 37°C | Homo sapiens | |
2.7.1.74 | 1.3 | - |
deoxycytidine | mutant R104Q/D133N, pH 7.5, 30°C | Homo sapiens | |
2.7.1.74 | 1.49 | - |
thymidine | mutant R104M/D133T, pH 7.5, 37°C | Homo sapiens | |
2.7.1.74 | 1.5 | - |
2'-deoxyguanosine | mutant R104Q/D133G, pH 7.5, 30°C | Homo sapiens | |
2.7.1.74 | 1.51 | - |
thymidine | mutant R104Q/D133N, pH 7.5, 30°C | Homo sapiens | |
2.7.1.74 | 1.57 | - |
thymidine | mutant A100V/R104M/D133T, pH 7.5, 37°C | Homo sapiens | |
2.7.1.74 | 1.7 | - |
deoxycytidine | mutant R104M/D133S, pH 7.5, 37°C | Homo sapiens | |
2.7.1.74 | 1.72 | - |
2'-deoxyguanosine | mutant A100V/R104M/D133S, pH 7.5, 37°C | Homo sapiens | |
2.7.1.74 | 1.75 | - |
2'-deoxyadenosine | mutant R104Q/D133G, pH 7.5, 30°C | Homo sapiens | |
2.7.1.74 | 1.88 | - |
thymidine | mutant R104M/D133A, pH 7.5, 37°C | Homo sapiens | |
2.7.1.74 | 1.91 | - |
thymidine | mutant A100V/R104M/D133S, pH 7.5, 37°C | Homo sapiens | |
2.7.1.74 | 1.92 | - |
2'-deoxyguanosine | mutant R104Q/D133N, pH 7.5, 30°C | Homo sapiens | |
2.7.1.74 | 2 | - |
2'-deoxyadenosine | mutant R104Q/D133N, pH 7.5, 30°C | Homo sapiens | |
2.7.1.74 | 2.1 | - |
deoxycytidine | mutant A100V/R104M/D133S, pH 7.5, 37°C | Homo sapiens | |
2.7.1.74 | 2.23 | - |
deoxycytidine | mutant R104M/D133N, pH 7.5, 37°C | Homo sapiens | |
2.7.1.74 | 2.27 | - |
thymidine | mutant R104M/D133N, pH 7.5, 37°C | Homo sapiens | |
2.7.1.74 | 2.29 | - |
2'-deoxyguanosine | mutant A100V/R104M/D133T, pH 7.5, 37°C | Homo sapiens | |
2.7.1.74 | 2.33 | - |
thymidine | mutant R104M/D133S, pH 7.5, 37°C | Homo sapiens | |
2.7.1.74 | 2.5 | - |
2'-deoxyguanosine | mutant R104M/D133T, pH 7.5, 37°C | Homo sapiens | |
2.7.1.74 | 2.67 | - |
2'-deoxyadenosine | mutant A100V/R104M/D133T, pH 7.5, 37°C | Homo sapiens | |
2.7.1.74 | 2.71 | - |
2'-deoxyadenosine | mutant R104M/D133A, pH 7.5, 37°C | Homo sapiens | |
2.7.1.74 | 2.86 | - |
deoxycytidine | mutant R104M/D133T, pH 7.5, 37°C | Homo sapiens | |
2.7.1.74 | 3.1 | - |
2'-deoxyadenosine | wild-type, pH 7.5, 37°C | Homo sapiens | |
2.7.1.74 | 3.1 | - |
2'-deoxyadenosine | wild-type, pH 7.5, 30°C | Homo sapiens | |
2.7.1.74 | 3.26 | - |
2'-deoxyguanosine | wild-type, pH 7.5, 37°C | Homo sapiens | |
2.7.1.74 | 3.26 | - |
2'-deoxyguanosine | wild-type, pH 7.5, 30°C | Homo sapiens | |
2.7.1.74 | 3.29 | - |
thymidine | mutant A100V/R104M/D133A, pH 7.5, 37°C | Homo sapiens | |
2.7.1.74 | 3.37 | - |
2'-deoxyadenosine | mutant A100V/R104M/D133S, pH 7.5, 37°C | Homo sapiens | |
2.7.1.74 | 3.4 | - |
2'-deoxyadenosine | mutant A100V/R104M/D133A, pH 7.5, 37°C | Homo sapiens | |
2.7.1.74 | 3.47 | - |
2'-deoxyadenosine | mutant R104M/D133N, pH 7.5, 37°C | Homo sapiens | |
2.7.1.74 | 3.54 | - |
deoxycytidine | mutant A100V/R104M/D133T, pH 7.5, 37°C | Homo sapiens | |
2.7.1.74 | 3.59 | - |
2'-deoxyguanosine | mutant R104M/D133N, pH 7.5, 37°C | Homo sapiens | |
2.7.1.74 | 4.14 | - |
2'-deoxyadenosine | mutant R104M/D133T, pH 7.5, 37°C | Homo sapiens | |
2.7.1.74 | 4.38 | - |
2'-deoxyguanosine | mutant R104M/D133S, pH 7.5, 37°C | Homo sapiens | |
2.7.1.74 | 4.85 | - |
2'-deoxyadenosine | mutant R104M/D133S, pH 7.5, 37°C | Homo sapiens |