Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Langley, D.B.; Shojaei, M.; Chan, C.; Lok, H.C.; Mackay, J.P.; Traut, T.W.; Guss, J.M.; Christopherson, R.I.
    Structure and inhibition of orotidine 5-monophosphate decarboxylase from Plasmodium falciparum (2008), Biochemistry, 47, 3842-3854.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.23 into the vector pET3d for expression in Escherichia coli cells Plasmodium falciparum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.1.23 the structure of PfODCase is solved to a resolution of 1.5418 A Plasmodium falciparum

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.23 6-azauridine 5'-monophosphate
-
 Plasmodium falciparum
4.1.1.23 allopurinol-3-riboside 5'-monophosphate
-
 Plasmodium falciparum
4.1.1.23 pyrazofurin 5'-monophosphate
-
 Plasmodium falciparum
4.1.1.23 xanthosine 5'-monophosphate
-
 Plasmodium falciparum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.23 0.00035
-
 orotidine 5'-phosphate
-
 Plasmodium falciparum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.23 38000
-
 subunit, determined by SDS-PAGE Plasmodium falciparum
4.1.1.23 76000
-
 determined by gel filtration Plasmodium falciparum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.23 Orotidine 5'-phosphate Plasmodium falciparum
-
 UMP + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.23 Plasmodium falciparum Q8T6J6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.23 using a Ni-NTA agarose column, the polyhistidine tag is removed with thrombin, the cleaved protein is further purified using a POROS-HS cation exchange and a Superdex-200 size-exclusion column Plasmodium falciparum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.23 Orotidine 5'-phosphate
-
 Plasmodium falciparum UMP + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.23 homodimer
-
 Plasmodium falciparum

Synonyms

EC Number Synonyms Comment Organism
4.1.1.23 Orotidine 5'-monophosphate decarboxylase
-
 Plasmodium falciparum
4.1.1.23 PfODCase
-
 Plasmodium falciparum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.23 37
-
 activity assay Plasmodium falciparum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.23 7.2
-
 activity assay Plasmodium falciparum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.1.1.23 0.0000036
-
 pyrazofurin 5'-monophosphate
-
 Plasmodium falciparum
4.1.1.23 0.0000044
-
 xanthosine 5'-monophosphate
-
 Plasmodium falciparum
4.1.1.23 0.000012
-
 6-azauridine 5'-monophosphate
-
 Plasmodium falciparum
4.1.1.23 0.00024
-
 allopurinol-3-riboside 5'-monophosphate
-
 Plasmodium falciparum