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Literature summary extracted from

  • Chakraborty, S.; Nemeria, N.; Yep, A.; McLeish, M.J.; Kenyon, G.L.; Jordan, F.
    Mechanism of benzaldehyde lyase studied via thiamin diphosphate-bound intermediates and kinetic isotope effects (2008), Biochemistry, 47, 3800-3809.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.38 the plasmid pKK-BAL-His is constructed for expression of wild-type BAL in Escherichia coli BL21DE3pLysS cells Pseudomonas fluorescens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.2.38 Mg2+
-
 Pseudomonas fluorescens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.2.38 58919
-
 4 * 58919 Pseudomonas fluorescens
4.1.2.38 58920
-
 monomer, calculated from sequence Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.2.38 2-hydroxy-1,2-diphenylethanone Pseudomonas fluorescens
-
 benzaldehyde
-
 r

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.38 Pseudomonas fluorescens P51853
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.38
-
 Pseudomonas fluorescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.38 (E)-2-oxo-4(pyridin-3-yl)-3-but-3-enoic acid
-
 Pseudomonas fluorescens (E)-3-(pyridine-3-yl)acrylaldehyde
-
 ?
4.1.2.38 2-hydroxy-1,2-diphenylethanone
-
 Pseudomonas fluorescens benzaldehyde
-
 r
4.1.2.38 benzoylformic acid
-
 Pseudomonas fluorescens ?
-
 ?
4.1.2.38 phenylpyruvic acid
-
 Pseudomonas fluorescens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.2.38 homotetramer 4 * 58919 Pseudomonas fluorescens

Synonyms

EC Number Synonyms Comment Organism
4.1.2.38 BAL
-
 Pseudomonas fluorescens
4.1.2.38 benzaldehyde lyase
-
 Pseudomonas fluorescens
4.1.2.38 benzoin aldolase
-
 Pseudomonas fluorescens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.2.38 30
-
 activity assay Pseudomonas fluorescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.2.38 8
-
 activity assay Pseudomonas fluorescens

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.2.38 thiamine diphosphate
-
 Pseudomonas fluorescens