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Literature summary extracted from

  • Nguyen, T.T.; Brown, S.; Fedorov, A.A.; Fedorov, E.V.; Babbitt, P.C.; Almo, S.C.; Raushel, F.M.
    At the periphery of the amidohydrolase superfamily: Bh0493 from Bacillus halodurans catalyzes the isomerization of D-galacturonate to D-tagaturonate (2008), Biochemistry, 47, 1194-1206.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.12 expressed in Escherichia coli BL21(DE3) cells Halalkalibacterium halodurans

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.3.1.12 complexed with Zn2+, vapour diffusion method, with 45% polypropylene glycol and 0.1 M Bis-Tris, pH 6.5 Halalkalibacterium halodurans

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.1.12 (2S,3R,4S)-4-(hydroxycarbamoyl)-2,3,4-trihydroxybutanoate
-
 Halalkalibacterium halodurans
5.3.1.12 D-arabinaric acid
-
 Halalkalibacterium halodurans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.12 0.05
-
 D-glucuronate at 30°C, pH 8.0 Halalkalibacterium halodurans
5.3.1.12 0.05
-
 D-galacturonate at 30°C, pH 8.0 Halalkalibacterium halodurans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.3.1.12 Zn2+ contains 0.5 equivalents of Zn2+ per subunit Halalkalibacterium halodurans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.1.12 49000
-
 SDS-PAGE Halalkalibacterium halodurans

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.12 Halalkalibacterium halodurans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.12 Resource Q column chromatography and Superdex 200 gel filtration Halalkalibacterium halodurans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.12 D-Galacturonate
-
 Halalkalibacterium halodurans D-Tagaturonate
-
 ?
5.3.1.12 D-Glucuronate
-
 Halalkalibacterium halodurans D-Fructuronate
-
 ?

Subunits

EC Number Subunits Comment Organism
5.3.1.12 trimer x-ray crystallography Halalkalibacterium halodurans

Synonyms

EC Number Synonyms Comment Organism
5.3.1.12 Bh0493
-
 Halalkalibacterium halodurans
5.3.1.12 Uronate isomerase
-
 Halalkalibacterium halodurans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.3.1.12 2.4
-
 D-galacturonate at 30°C, pH 8.0 Halalkalibacterium halodurans
5.3.1.12 5.2
-
 D-glucuronate at 30°C, pH 8.0 Halalkalibacterium halodurans

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
5.3.1.12 0.0021
-
 (2S,3R,4S)-4-(hydroxycarbamoyl)-2,3,4-trihydroxybutanoate at 30°C, pH 8.0 Halalkalibacterium halodurans
5.3.1.12 0.0055
-
 D-arabinaric acid at 30°C, pH 8.0 Halalkalibacterium halodurans