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Literature summary extracted from

  • Du, L.; He, Y.; Luo, Y.
    Crystal Structure and Enantiomer Selection by D-Alanyl Carrier Protein Ligase DltA from Bacillus cereus (2008), Biochemistry, 47, 11473-11480.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.2.1.54 expressed in Escherichia coli as a His-tagged fusion protein Bacillus cereus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.2.1.54 crystal structure of a DltA is determined at 2.0 A resolution in complex with the D-alanine adenylate intermediate of the first reaction. Despite the low level of sequence similarity, the DltA structure resembles known structures of adenylation domains such as the acetyl-CoA synthetase. The enantiomer selection appears to be enhanced by the medium-sized side chain of Cys-269 Bacillus cereus

Protein Variants

EC Number Protein Variants Comment Organism
6.2.1.54 C269A C269A mutation relaxes D-Ala preference. Km (mM): (D-Ala) 3.1, (L-Ala) 6.6, kcat (1/sec): (D-Ala) 0.103, (L-Ala) 0.12 Bacillus cereus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.54 1.1
-
 D-Ala wild-tpye Bacillus cereus
6.2.1.54 3.1
-
 D-Ala mutant C269A Bacillus cereus
6.2.1.54 6.6
-
 L-Ala mutant C269A Bacillus cereus
6.2.1.54 14.4
-
 L-Ala wild-tpye Bacillus cereus

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.54 Bacillus cereus Q81G39
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.54 using Ni-NTA chromatography Bacillus cereus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.54 ATP + D-Ala + diphosphate
-
 Bacillus cereus ?
-
 ?
6.2.1.54 ATP + L-Ala + diphosphate
-
 Bacillus cereus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.2.1.54 BcDltA
-
 Bacillus cereus
6.2.1.54 D-alanyl carrier protein ligase
-
 Bacillus cereus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.2.1.54 0.025
-
 D-Ala wild-tpye Bacillus cereus
6.2.1.54 0.103
-
 D-Ala mutant C269A Bacillus cereus
6.2.1.54 0.11
-
 L-Ala wild-tpye Bacillus cereus
6.2.1.54 0.12
-
 L-Ala mutant C269A Bacillus cereus