EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.7.1.107 | C46A/C113A | mutant lacking all Cys residues. Activity is slightly higher than wild-type | Escherichia coli |
2.7.1.107 | C46A/C113A/A29C | introduction of Cys residue at transmembrane helix 1 into mutant lacking the native Cys residues. Low activity mutant, 64% trimer formation compared to wild-type | Escherichia coli |
2.7.1.107 | C46A/C113A/A30C | introduction of Cys residue at transmembrane helix 1 into mutant lacking the native Cys residues. Low activity mutant, 79% trimer formation compared to wild-type | Escherichia coli |
2.7.1.107 | C46A/C113A/E28C | introduction of Cys residue at transmembrane helix 1 into mutant lacking the native Cys residues. Low activity mutant, 93% trimer formation compared to wild-type | Escherichia coli |
2.7.1.107 | C46A/C113A/Q33C | introduction of Cys residue at transmembrane helix 1 into mutant lacking the native Cys residues. Low activity mutant, 77% trimer formation compared to wild-type | Escherichia coli |
2.7.1.107 | C46A/C113A/R32C | introduction of Cys residue at transmembrane helix 1 into mutant lacking the native Cys residues. Low activity mutant, 63% trimer formation compared to wild-type | Escherichia coli |
2.7.1.107 | C46A/C113AE34C | introduction of Cys residue at transmembrane helix 1 into mutant lacking the native Cys residues. Low activity mutant, 100% trimer formation compared to wild-type | Escherichia coli |
2.7.1.107 | C46A/C113AF31C | introduction of Cys residue at transmembrane helix 1 into mutant lacking the native Cys residues. Low activity mutant, 72% trimer formation compared to wild-type | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.107 | additional information | - |
additional information | Km-value in mol% for wild-type 9.2, mutant C46A/C113A 4.9, mutant C46A/C113A/E28C 5.7, mutant C46A/C113A/A29C 7.4, mutant C46A/C113AA30C 27.2, mutant C46A/C113A/F31C 17.2, mutant C46A/C113A/R32C 12.9, mutant C46A/C113A/Q33C 12.1, mutant C46A/C113A/E34C 13.7 | Escherichia coli | |
2.7.1.107 | 0.13 | - |
ATP | mutant C46A/C113A, pH 6.9, 25°C | Escherichia coli | |
2.7.1.107 | 0.34 | - |
ATP | wild-type, pH 6.9, 25°C | Escherichia coli | |
2.7.1.107 | 0.4 | - |
ATP | mutant C46A/C113A/Q33C, pH 6.9, 25°C | Escherichia coli | |
2.7.1.107 | 0.6 | - |
ATP | mutant C46A/C113A/E34C, pH 6.9, 25°C | Escherichia coli | |
2.7.1.107 | 1 | - |
ATP | mutant C46A/C113A/A29C, pH 6.9, 25°C | Escherichia coli | |
2.7.1.107 | 1.3 | - |
ATP | mutant C46A/C113A/E28C, pH 6.9, 25°C | Escherichia coli | |
2.7.1.107 | 2.1 | - |
ATP | mutant C46A/C113A/F31C, pH 6.9, 25°C | Escherichia coli | |
2.7.1.107 | 3.3 | - |
ATP | mutant C46A/C113AA30C, pH 6.9, 25°C | Escherichia coli | |
2.7.1.107 | 4.8 | - |
ATP | mutant C46A/C113A/R32C, pH 6.9, 25°C | Escherichia coli |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.7.1.107 | membrane | the region of transmembrane helix 1 spanning the hydrophobic core of the bilayer runs from Glu28 on the cytoplasmic side to Asp49 or Val50 on the periplasmic side. This locates the charged/polar cluster 32RQE34 within the hydrophobic core of the bilayer. Hydrophobic matching between the protein and the surrounding lipid bilayer is highly efficient | Escherichia coli | 16020 | - |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.107 | Escherichia coli | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.107 | ATP + 1,2-dihexanoylglycerol | - |
Escherichia coli | ADP + 1,2-dihexanoylglycerol 3-phosphate | - |
? |