EC Number | Application | Comment | Organism |
---|---|---|---|
4.4.1.5 | analysis | practical experiments for students to determine the effect of exposure to oxidative stress conditions on yeast glyoxalase I, investigation of kinetic parameters | Saccharomyces cerevisiae |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.4.1.5 | 1.03 | - |
methylglyoxal | the apparent kinetic parameters are determined by varying the hemithioacetal concentration between 0.3 and 1.5 mM, which is obtained by the nonenzymatic reaction between glutathione and methylglyoxal | Saccharomyces cerevisiae |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.4.1.5 | Saccharomyces cerevisiae | - |
permeabilized with 0.01% (w/v) digitonin | - |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
4.4.1.5 | additional information | permeabilized cells of Saccharomyces cerevisiae, with 0.01% (w/v) digitonin | Saccharomyces cerevisiae | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.4.1.5 | glutathione + 2-oxopropanal | - |
Saccharomyces cerevisiae | (R)-S-lactoylglutathione | - |
r | |
4.4.1.5 | glutathione + methylglyoxal | - |
Saccharomyces cerevisiae | S-D-lactoylglutathione | - |
? | |
4.4.1.5 | glutathione + methylglyoxal | glutathione reacts with methylglyoxal forming a hemithioacetal, and then glyoxlase I catalyses the formation of S-D-lactoylglutathione | Saccharomyces cerevisiae | S-((R)-lactoyl)glutathione | - |
? | |
4.4.1.5 | additional information | the glyoxalase I activity is measured spectrophotometrically by S-D-lactoylglutathione formation at 240 nm | Saccharomyces cerevisiae | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.4.1.5 | glyoxalase I | - |
Saccharomyces cerevisiae |
4.4.1.5 | S-D-lactoylglutathione:methylglyoxal lyase | - |
Saccharomyces cerevisiae |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.4.1.5 | 258.3 | - |
glutathione | the apparent kinetic parameters are determined by using hemithioacetal as substrate, which is obtained by the nonenzymatic reaction between glutathione and methylglyoxal | Saccharomyces cerevisiae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.4.1.5 | 6.5 | - |
assay at | Saccharomyces cerevisiae |