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Literature summary extracted from

  • Izumikawa, T.; Uyama, T.; Okuura, Y.; Sugahara, K.; Kitagawa, H.
    Involvement of chondroitin sulfate synthase-3 (chondroitin synthase-2) in chondroitin polymerization through its interaction with chondroitin synthase-1 or chondroitin-polymerizing factor (2007), Biochem. J., 403, 545-552.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.226 chondroitin synthase-1 co-expressed with chondroitin synthase-1 CSS3 also exhibits polymerase activity Homo sapiens
2.4.1.226 chondroitin-polymerizing factor co-expressed with ChPF (chondroitin-polymerizing factor) enzyme shows chondroitin polymerase activity Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.175 expressed in HeLa cells Homo sapiens
2.4.1.175 soluble form of the enzyme lacking the first 129 amino acids expressed as protein A fusion protein in COS-1 cells Homo sapiens
2.4.1.226 expressed in COS-1 cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.226 D184A mutant protein which lacks any glycosyltransferase activity but interacts with other ChSy family members shows that the glycosyltransferase activity of CSGlcA-T plays an important role in chondroitin polymerization Homo sapiens
2.4.1.226 additional information overexpression of CSGlcA-T increases the amount of chondroitin sulfate in HeLa cells, whereas the RNA interference of CSGlcA-T results in a reduction of the amount of chondroitin sulphate in the cells Homo sapiens
2.4.1.226 additional information overexpression of CSS3 increases the amount of chondroitin sulfate in HeLa cells, while the RNA interference of CSS3 results in a reduction in the amount of CS in the cells Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.226 Golgi apparatus
-
 Homo sapiens 5794
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.226 Mg2+
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.175 additional information Homo sapiens possess N-acetylgalactosaminyltransferase-II and glucuronyltransferase-II activities responsible for the elongation of chondroitin sulfate chains but cannot polymerize chondroitin chains by themselves ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.175 Homo sapiens A2V663
-
-
2.4.1.226 Homo sapiens
-
-
-
2.4.1.226 Homo sapiens A2V663
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.226 a soluble form of CSGlcA-T is generated by replacing the first 57 amino acids of the protein with a cleavable insulin signal sequence and a protein A IgG-binding domain. The soluble protein is expressed in COS-1 cells as a recombinant protein fused with the protein A IgG-binding domain. The fusion protein secreted into the medium is adsorbed onto IgG-Sepharose beads for purification to eliminate endogenous glycosyltransferases, and then the protein-bound beads are used as an enzyme source Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.175 brain
-
 Homo sapiens
-
2.4.1.175 colon
-
 Homo sapiens
-
2.4.1.175 heart
-
 Homo sapiens
-
2.4.1.175 kidney
-
 Homo sapiens
-
2.4.1.175 leukocyte from peripheral blood Homo sapiens
-
2.4.1.175 liver
-
 Homo sapiens
-
2.4.1.175 lung
-
 Homo sapiens
-
2.4.1.175 placenta
-
 Homo sapiens
-
2.4.1.175 skeletal muscle
-
 Homo sapiens
-
2.4.1.175 small intestine
-
 Homo sapiens
-
2.4.1.175 spleen
-
 Homo sapiens
-
2.4.1.175 thymus
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.175 alpha-thrombomodulin + UDP-N-acetyl-D-galactosamine + UDP-glucuronic acid
-
 Homo sapiens ?
-
 ?
2.4.1.175 additional information possess N-acetylgalactosaminyltransferase-II and glucuronyltransferase-II activities responsible for the elongation of chondroitin sulfate chains but cannot polymerize chondroitin chains by themselves Homo sapiens ?
-
 ?
2.4.1.226 additional information CSGlcA-T exhibits polymerization activity on alpha-thrombomodulin using alpha-thrombomodulin as acceptor incubated with UDP-Gal-NAc and UDP-GlcUA. The chain length of chondroitin formed by the co-expressed proteins ChSy-1,ChSy-2 (CSS3), or ChPF in various combinations is different Homo sapiens ?
-
 ?
2.4.1.226 additional information polymerization reactions carried out using alpha-thrombomodulin as an acceptor co-incubated with UDP-GalNAc and UDP-GlcA Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.175 chondroitin sulfate synthase-3
-
 Homo sapiens
2.4.1.175 chondroitin synthase-2 based on the experimental results chondroitin sulfate synthase-3 is renamed into chondroitin synthase-2 Homo sapiens
2.4.1.175 ChSy-2
-
 Homo sapiens
2.4.1.175 CSS3
-
 Homo sapiens
2.4.1.226 chondroitin sulfate glucoronyltransferase
-
 Homo sapiens
2.4.1.226 chondroitin sulfate synthase-3 enzyme possesses dual enzymatic GalNAcT-II (N-acetylgalactosaminyltransferase-II) and GlcAT-II (glucuronyltransferase-II) activities Homo sapiens
2.4.1.226 chondroitin synthase-3
-
 Homo sapiens
2.4.1.226 ChSy-3
-
 Homo sapiens
2.4.1.226 CSGlcA-T enzyme posseses weak GlcAT-II and GalNAcT-II activities Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.226 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.226 6.5
-
 assay at Homo sapiens