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Literature summary extracted from

  • Mocan, I.; Georgescauld, F.; Gonin, P.; Thoraval, D.; Cervoni, L.; Giartosio, A.; Dabernat-Arnaud, S.; Crouzet, M.; Lacombe, M.; Lascu, I.
    Protein phosphorylation corrects the folding defect of the neuroblastoma (S120G) mutant of human nucleoside diphosphate kinase A/Nm23-H1 (2007), Biochem. J., 403, 149-156.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.4.6 expression of wild-type and mutant enzymes Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.7.4.6 H118N/S120G site-directed mutagenesis, the mutant is inactive and expressed as insoluble protein in inclusion bodies Homo sapiens
2.7.4.6 S120G naturally mutation occuring in neuroblastoma, the mutant has a folding defect, the urea-denatured protein cannot refold in vitro Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.6 Mg2+ MgATP promotes correct folding and association Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.4.6 ATP + NDP Homo sapiens MgATP promotes correct folding and association ADP + NTP
-
 ?
2.7.4.6 additional information Homo sapiens NDPKA is a housekeeping enzyme essential for the synthesis of nonadenine nucleoside and deoxynucleoside 5'-triphosphate ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.6 Homo sapiens P15531
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.4.6 phosphoprotein the molten globule folding intermediate can bind ATP and be autophosphorylated Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.6 recombinant wild-type and mutant enzymes by anion exchange chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.4.6 neuroblastoma cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.6 ATP + NDP
-
 Homo sapiens ADP + NTP
-
 ?
2.7.4.6 ATP + NDP MgATP promotes correct folding and association Homo sapiens ADP + NTP
-
 ?
2.7.4.6 additional information NDPKA is a housekeeping enzyme essential for the synthesis of nonadenine nucleoside and deoxynucleoside 5'-triphosphate Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.4.6 hexamer MgATP promotes correct folding and association, but MgATP does not shift the equilibrium between the folding intermediate and the native monomer in the presence of 2-3 M urea. The molten globule folding intermediate can bind ATP and be autophosphorylated Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.7.4.6 NDP kinase A
-
 Homo sapiens
2.7.4.6 NDPKA
-
 Homo sapiens
2.7.4.6 Nm23-H1
-
 Homo sapiens
2.7.4.6 nucleoside diphosphate kinase A
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.4.6 25
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.4.6 8
-
 assay at Homo sapiens