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Literature summary extracted from
- Spatial subunit distribution and in vitro functions of the novel trimeric PCNA complex from Sulfolobus tokodaii (2008), Biochem. Biophys. Res. Commun., 376, 369-374.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 6.5.1.1 | expression of His-tagged DNA ligase I in Escherichia coli strain BL21(DE3) | Sulfurisphaera tokodaii |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 6.5.1.1 | EDTA | - |
Sulfurisphaera tokodaii |  |
| 6.5.1.1 | PCNA | inhibition of the DNA ligase I activity by proliferating cell nuclear antigen, PCNA, complexes StoPCNA. StoPCNA1 and StoPCNA3, overview | Sulfurisphaera tokodaii |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 6.5.1.1 | Mg2+ | - |
Sulfurisphaera tokodaii | |
Organic Solvent Stability
| EC Number | Organic Solvent | Comment | Organism |
|---|---|---|---|
| 6.5.1.1 | formamide | inactivation at about 50% | Sulfurisphaera tokodaii |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 6.5.1.1 | Sulfurisphaera tokodaii | Q976G4 | - |
- |
| 6.5.1.1 | Sulfurisphaera tokodaii 7 | Q976G4 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 6.5.1.1 | recombinant His-tagged DNA ligase I from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration | Sulfurisphaera tokodaii |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 6.5.1.1 | ATP + DNA substrate S4 | - |
Sulfurisphaera tokodaii | AMP + diphosphate + ? | - |
? | |
| 6.5.1.1 | ATP + DNA substrate S4 | - |
Sulfurisphaera tokodaii 7 | AMP + diphosphate + ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 6.5.1.1 | DNA ligase I | - |
Sulfurisphaera tokodaii |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 6.5.1.1 | 60 | - |
assay at | Sulfurisphaera tokodaii |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 6.5.1.1 | 8 | - |
assay at | Sulfurisphaera tokodaii |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 6.5.1.1 | ATP | - |
Sulfurisphaera tokodaii | |
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Release 2023.1 (January 2023)
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