Literature summary extracted from

Mukai, T.; Kobayashi, T.; Hino, N.; Yanagisawa, T.; Sakamoto, K.; Yokoyama, S.
Adding l-lysine derivatives to the genetic code of mammalian cells with engineered pyrrolysyl-tRNA synthetases (2008), Biochem. Biophys. Res. Commun., 371, 818-822.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
6.1.1.26	expression of tRNAPyl in Chinese hamster ovary cells. ZLysRS-tRNAPyl pair and GRB2(Am111)-FLAG expressed in HEK293 c-18 cells	Methanosarcina mazei

Protein Variants

EC Number	Protein Variants	Comment	Organism
6.1.1.26	L309A/C348V	designated as ZLysRS has the L309A and C348V substitutions at the pyrrolysine binding pocket and three mutations at the other sites	Methanosarcina mazei
6.1.1.26	additional information	four substitutions (L305I, Y306F, L309A, and C348F) at the binding pocket out of the six mutations found in AcLysRS from Methanosarcina barkeri are transplanted into Methanosarcina mazei PylRS to create mAcLysRS	Methanosarcina mazei

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
6.1.1.26	ATP + L-pyrrolysine + tRNAPyl	Methanosarcina mazei	-	AMP + diphosphate + L-pyrrolysyl-tRNAPyl	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
6.1.1.26	Methanosarcina mazei	-	-	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
6.1.1.26	ATP + Boc-lysine + tRNAPyl	Nepsilon-tert-butyloxycarbonyl-L-lysine	Methanosarcina mazei	?	-	?
6.1.1.26	ATP + L-pyrrolysine + tRNAPyl	-	Methanosarcina mazei	AMP + diphosphate + L-pyrrolysyl-tRNAPyl	-	?
6.1.1.26	ATP + N-alpha-acetyl-L-lysine + tRNAPyl	-	Methanosarcina mazei	?	-	?
6.1.1.26	ATP + N-alpha-benzyloxycarbonyl-L-lysine + tRNAPyl	-	Methanosarcina mazei	?	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
6.1.1.26	pyrrolysyl-tRNA synthetase	-	Methanosarcina mazei

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Release 2023.1 (January 2023)