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Literature summary extracted from

  • Kino, K.; Nakazawa, Y.; Yagasaki, M.
    Dipeptide synthesis by L-amino acid ligase from Ralstonia solanacearum (2008), Biochem. Biophys. Res. Commun., 371, 536-540.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.2.49 gene lal, expression of His-tagged enzyme in Escherichia coli Ralstonia solanacearum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.2.49 additional information
-
 additional information Michaelis-Menten kinetics Ralstonia solanacearum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.2.49 Mg2+
-
 Ralstonia solanacearum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.2.49 49500
-
 x * 49500, recombinant His-tagged enzyme, SDS-PAGE Ralstonia solanacearum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.2.49 ATP + an L-amino acid + an L-amino acid Ralstonia solanacearum
-
 ADP + phosphate + L-aminoacyl-L-amino acid
-
 ?
6.3.2.49 ATP + an L-amino acid + an L-amino acid Ralstonia solanacearum JCM 10489
-
 ADP + phosphate + L-aminoacyl-L-amino acid
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.49 Ralstonia solanacearum B2DEW2 gene lal
-
6.3.2.49 Ralstonia solanacearum JCM 10489 B2DEW2 gene lal
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.49 recombinant His-tagged enzyme from Escherichia coli Ralstonia solanacearum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.49 ATP + an L-amino acid + an L-amino acid
-
 Ralstonia solanacearum ADP + phosphate + L-aminoacyl-L-amino acid
-
 ?
6.3.2.49 ATP + an L-amino acid + an L-amino acid substrate and dipeptide formation specificity analysis, the enzyme shows high activity with L-Phe, L-Met, and L-His, detailed overview. RSp1486a exhibits different substrate specificity from Bacillus subtilus Lal, and preferably synthesizes hetero-dipeptides where a more bulky amino acid is placed at the N-terminus and a less bulky amino acid is placed at the C-terminus in contrast to those synthesized by Bacillus subtilus Lal, overview Ralstonia solanacearum ADP + phosphate + L-aminoacyl-L-amino acid
-
 ?
6.3.2.49 ATP + an L-amino acid + an L-amino acid
-
 Ralstonia solanacearum JCM 10489 ADP + phosphate + L-aminoacyl-L-amino acid
-
 ?
6.3.2.49 ATP + an L-amino acid + an L-amino acid substrate and dipeptide formation specificity analysis, the enzyme shows high activity with L-Phe, L-Met, and L-His, detailed overview. RSp1486a exhibits different substrate specificity from Bacillus subtilus Lal, and preferably synthesizes hetero-dipeptides where a more bulky amino acid is placed at the N-terminus and a less bulky amino acid is placed at the C-terminus in contrast to those synthesized by Bacillus subtilus Lal, overview Ralstonia solanacearum JCM 10489 ADP + phosphate + L-aminoacyl-L-amino acid
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.2.49 ? x * 49500, recombinant His-tagged enzyme, SDS-PAGE Ralstonia solanacearum

Synonyms

EC Number Synonyms Comment Organism
6.3.2.49 LAL
-
 Ralstonia solanacearum
6.3.2.49 RSp1486a
-
 Ralstonia solanacearum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.2.49 37
-
 assay at Ralstonia solanacearum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.2.49 8
-
 assay at Ralstonia solanacearum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.3.2.49 6 10
-
 Ralstonia solanacearum

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.2.49 ATP
-
 Ralstonia solanacearum