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Literature summary extracted from

  • Wei, T.; Zhang, S.; Zhu, S.; Sheng, D.; Ni, J.; Shen, Y.
    Physical and functional interaction between archaeal single-stranded DNA-binding protein and the 5'-3' nuclease NurA (2008), Biochem. Biophys. Res. Commun., 367, 523-529.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.16.1 gene nurA, DNA and amino acid sequence determination and analysis, the nurA gene is arranged in an operon-like structure with mre11, rad50, and herA genes, expression with StoSSB in the two-hybrid system in yeast AH109 Sulfurisphaera tokodaii

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.16.1 single-stranded DNA-binding protein i.e. StoSSB, from the hyperthermophilic archaeon Sulfolobus tokodaii, StoSSB inhibits the exonuclease activity of StoNurA on ssDNA and dsDNA Sulfurisphaera tokodaii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.16.1 Mg2+
-
 Sulfurisphaera tokodaii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.16.1 additional information Sulfurisphaera tokodaii NurA may function closely together with SSB in DNA transactions in archaea ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.16.1 Sulfurisphaera tokodaii
-
 gene nurA
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.16.1 additional information NurA may function closely together with SSB in DNA transactions in archaea Sulfurisphaera tokodaii ?
-
 ?
3.1.16.1 additional information NurA has ssDNA endonuclease, 5'-3' ssDNA exonuclease, and 5'-3' dsDNA exonuclease activities, overview. NurA is closely linked to Mre11 and Rad50 homologues, it interacts with single-stranded DNA-binding protein, StoSSB, from the hyperthermophilic archaeon Sulfolobus tokodaii, determination by pull-down assay using Ni-NTA agarose beads and MALDI-TOF mass spectrometry, and yeast two-hybrid and co-immunoprecipitation analysis, overview. Substrates are 34-bp dsDNA and M13mp18 ssDNA Sulfurisphaera tokodaii ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.16.1 22
-
 assay at room temperature Sulfurisphaera tokodaii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.16.1 8
-
 assay at Sulfurisphaera tokodaii