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Literature summary extracted from

  • Bellelli, A.; Cavallo, S.; Nicolini, L.; Cervelli, M.; Bianchi, M.; Mariottini, P.; Zelli, M.; Federico, R.
    Mouse spermine oxidase: a model of the catalytic cycle and its inhibition by N,N1-bis(2,3-butadienyl)-1,4-butanediamine (2004), Biochem. Biophys. Res. Commun., 322, 1-8.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.3.16 expression in Escherichia coli BL21 DE3 Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.3.16 N,N1-bis(2,3-butadienyl)-1,4-butanediamine i.e. MDL72527, competitive. If incubated for longer times, MDL72527 yields irreversible inhibition of the enzyme with a half-life of 15 min at 0.1 mM MDL72527 Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.3.16 0.17
-
 spermine
-
 Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.3.16 63000
-
 x * 63000, SDS-PAGE Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
1.5.3.16 Mus musculus Q99K82
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.3.16 recombinant enzyme Mus musculus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.3.16 5
-
-
 Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.3.16 additional information no activity with spermidine and putrescine Mus musculus ?
-
 ?
1.5.3.16 spermine + O2 + H2O the mMSO catalytic mechanism is consistent with a simple four-step kinetic scheme. The enzyme is unable to oxidize other free or acetylated polyamines Mus musculus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.3.16 ? x * 63000, SDS-PAGE Mus musculus

Synonyms

EC Number Synonyms Comment Organism
1.5.3.16 mSMO
-
 Mus musculus
1.5.3.16 spermine oxidase
-
 Mus musculus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.3.16 4.8
-
 spermine
-
 Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.3.16 8.5
-
-
 Mus musculus

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.3.16 FAD non-covalently bound to the enzyme Mus musculus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.5.3.16 0.063
-
 N,N1-bis(2,3-butadienyl)-1,4-butanediamine
-
 Mus musculus